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1

artículo

A PCR Test Using the Mini-PCR Platform and Simplified Product Detection Methods Is Highly Sensitive and Specific to Detect Fasciola hepatica DNA Mixed in Human Stool, Snail Tissue,...

Publicado por
Fernandez-Baca, M.V., Castellanos-González, A., Ore, R.A., Alccacontor-Munoz, J.L., Hobán-Vergara, C., Castro, C.A., Tanabe, M.B., Morales, M.L., Ortiz-Oblitas, P., White, A.C., Cabada, M.M.

Publicado 2024

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Fasciola hepatica has a complex lifecycle with multiple intermediate and definitive hosts and influenced by environmental factors. The disease causes significant morbidity in children and its prevalent worldwide. There is lack of data about distribution and burden of the disease in endemic regions, owing to poor efficacy of the different diagnostic methods used. A novel PCR-based test was developed by using a portable mini-PCR® platform to detect Fasciola sp. DNA and interpret the results via a fluorescence viewer and smartphone image analyzer application. Human stool, snail tissue, and water samples were used to extract DNA. Primers targeting the ITS-1 of the 18S rDNA gene of Fasciola sp. were used. The limit of detection of the mini-PCR test was 1 fg/μL for DNA samples diluted in water, 10 fg/μL for Fasciola/snail DNA scramble, and 100 fg/μL for Fasciola/stool DNA scramble. The pro...

2

artículo

Neovascular Glaucoma as a Predictor of Retinoblastoma High-Risk Histopathology in an International Multicentre Study

Publicado por
Negretti, GS, Ushakova, T, Yuri, S, Vladimir, P, Berry, JL, Pike, S, Shields, CL, Hubbard, GB,, III, Eiger-Moscovich, M, Pe'er, J, Staffieri, SE, Elder, JE, McKenzie, JD, Ahmad, A, Hussain, M, Casavilca-Zambrano, S, Alarcon-Leon, S, Yousef, YA, Mohammad, M, Tanabe, M, Arazi, M, Fabian, ID, Goldstein, S, Kaliki, S, Sagoo, MS, Reddy, MA

Publicado 2024

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Purpose: To assess histopathology and outcomes following primary enucleation of eyes with retinoblastoma presenting with neovascular glaucoma (NVG). Methods: This was an international multi-centre case series study across five continents. Retrospective review of patient charts was performed for all patients undergoing primary enucleation for retinoblastoma (n=1420) using a standardised data-collection spreadsheet. Clinical features, pathological grade, and outcomes were compared between NVG patients and those with an American Joint Commission on Cancer (AJCC) 8th edition clinical stage of cT2. High-risk histopathology was defined as AJCC 8th edition pathological stage ≥pT2b. Results: NVG was seen in 224/1420 (16%) patients. Mean age at presentation of those with NVG was 30 months (median 25, range 0-120 months) and 131(58%) patients had high-risk histopathology. The univariate logistic...

3

artículo

Treatment Outcomes and Definition Inconsistencies in High-Risk Unilateral Retinoblastoma

Publicado por
Arazi, M, Baum, A, Casavilca-Zambrano, S, Alarcon-Leon, S, Diaz-Coronado, R, Ahmad, Alia, Mushtaq, Asma, Hussain, M, Ushakova, T, Yuri, S, Vladimir, P, Shields, Cl, Eagle, RC, Berry, JL, Pike, S, Brown, B, Roy, Sr, Huque, F, Fabian, Ina, Frenkel, S, Eiger-Moscovich, Maya, Pe'er, J, Hubbard, GB, Olson, TA, Grossniklaus, H, Reddy, MA, Sagoo, MA, Staffieri, SE, Elder, JE, Mckenzie, JD, Tanabe, M, Kaliki, S, Fabian, ID

Publicado 2024

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Purpose: To compare the clinical outcomes of children with unilateral retinoblastoma (Rb) and high-risk histopathology features (HRHF) following upfront enucleation with/without adjuvant chemotherapy, and investigate cases locally considered non-HRHF but converted to a standardized HRHF definition. Design: Retrospective multinational clinical cohort study. Methods: Children with Rb who presented to 21 centers from 12 countries between 2011-2020, and underwent primary enucleation were recruited. Centers retrieved clinical data and were asked to report detailed histopathology findings, as well as indicate cases defined locally as high-risk. For analysis, only unilateral cases with standardized HRHF, defined as retrolaminar optic nerve invasion, massive choroidal invasion, scleral invasion, anterior-segment involvement, and/or combined nonmassive choroidal and prelaminar/laminar optic nerve...

4

artículo

Retinoblastoma Outcomes Based on the 8th Edition American Joint Committee on Cancer Pathological Classification in 1411 Patients

Publicado por
Vempuluru, VS, Shields, CL, Berry, JL, Kaliki, S, Ahmad, A, Bejjanki, KM, Diaz-Coronado, R, Eiger-Moscovich, M, Elder, JE, Fabian, ID, Frenkel, S, Grossniklaus, H, Hubbard, GB,, III, Kapoor, AG, Mohammad, M, McKenzie, JD, Pe'er, J, Rath, S, Reddy, MA, Rolfe, O, Roy, SR, Sagoo, MS, Staffieri, SE, Tanabe, M, Tatiana, U, Tripathy, D, Vishnevskia-Dai, V, Vladimir, P, Yousef, YA

Publicado 2024

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Purpose: To evaluate the outcomes of retinoblastoma (RB) based on the 8th edition of the American Joint Committee on Cancer (AJCC) pathological classification in a global cohort of patients. Design: Retrospective, multicenter, intercontinental, collaborative study. Participants: A total of 1411 patients. Intervention: Primary enucleation with or without adjuvant chemotherapy or radiotherapy. Main Outcome Measures: Orbital tumor recurrence, tumor-related metastasis, and tumor-related death. Results: According to the 8th edition AJCC pathological classification, 645 eyes (46%) belonged to pathological T (pT)1, 164 (11%) to pT2, 493 (35%) to pT3, and 109 (8%) to pT4 categories. At a mean follow-up of 38 months (median, 35 months; < 1–149 months), orbital tumor recurrence was seen in 8 (1%), 5 (3%), 22 (4%), and 25 (23%) of pT1, pT2, pT3, and pT4 (P < 0.001) categories, respectively;...

5

artículo

High-risk histopathological features of retinoblastoma following primary enucleation: A Global Study Of 1,426 Patients From 5 Continents

Publicado por
Kaliki, S, Vempuluru, VS, Bakal, KR, Dorji, S, Tanna, V, Shields, CN, Fallon, SJ, Raval, V, Ahmad, A, Mushtaq, A, Hussain, M, Yousef, YA, Mohammad, M, Roy, SR, Huque, F, Tatiana, U, Yuri, S, Vladimir, P, Zambrano, SC, Alarcón-León, S, Valdiviezo-Zapata, C, Vargas-Martorellet, M, Gutierrez-Chira, C, Buitrago, M, Ortiz, JS, Diaz-Coronado, R, Tripathy, D, Rath, S, Patil, G, Berry, JL, Pike, S, Brown, B, Tanabe, M, Frenkel, S, Eiger-Moscovich, M, Pe'er, J, Shields, CL, Eagle, RC,, Jr, Laiton, A, Velasco, AM, Vega, K, Desimone, J, Bejjanki, KM, Kapoor, AG, Venkataraman, A, Bryant, V, Reddy, MA, Sagoo, MS, Hubbard, GB, Azarcon, CP, Olson, TA, Grossniklaus, H, Rolfe, O, Staffieri, SE, O'day, R, Mathew, AA, Elder, JE, Mckenzie, JD, Fabian, ID, Shemesh, R, Vishnevskia-Dai, V, Ali, MH, Jakati, S, Mishra, DK, Palkonda, VAR

Publicado 2024

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Purpose: To evaluate high-risk histopathological features following primary enucleation of eyes with retinoblastoma and assess the patient outcomes across continents. Methods: A retrospective study of 1,426 primarily enucleated retinoblastoma eyes from five continents. Results: Of all, 923 (65%) were from Asia (AS), 27 (2%) from Australia (AUS), 120 (8%) from Europe (EUR), 162 (11%) from North America (NA), and 194 (14%) from South America (SA). Based on the continent (AS vs. AUS vs. EUR vs. NA vs. SA), the histopathological features included massive choroidal invasion (31% vs. 7% vs. 13% vs. 19% vs. 27%, P = 0.001), postlaminar optic nerve invasion (27% vs. 0% vs. 16% vs. 21% vs. 19%, P = 0.0006), scleral infiltration (5% vs. 0% vs. 4% vs. 2% vs. 7%, P = 0.13), and microscopic extrascleral infiltration (4% vs. 0% vs. <1% vs. <1% vs. 4%, P = 0.68). Adjuvant chemotherapy with/withou...