The rocoto (Capsicum pubescens Ruiz & Pav.) is a nightshade with a center of origin in Peru, with great gastronomic value. It is affected by diseases that limit its development, spread by infected seeds. The in vitro culture of plant tissues allows the propagation of elite genotypes with the availability of pathogen-free plant material. The objective was to determine the in vitro response of hot pepper in the establishment of seeds and regeneration from terminal buds. Hot pepper seeds were used in different treatments with sodium hypochlorite. Leaves and roots of germinated rocotts were removed in vitro, leaving epicotyl with terminal buds and transferred to culture media with different concentrations of salts and MS vitamins for the in vitro regeneration phase, the organogenic response and oxidation were evaluated. In the establishment of hot pepper seeds, 100% disinfection was obta...

Myrciaria dubia (Kunth) McVaugh (camu-camu) is a shrub native to the Amazon region that produces fruits with a high content of vitamin C and various bioactive compounds, making it a functional food with antioxidant, anti-inflammatory, and antimicrobial properties. However, it is unknown which microorganisms are associated with its root system and can influence its growth and productivity. Arbuscular mycorrhizal fungi (AMF) are associated with most plants and are essential for their establishment, survival, and productivity since they facilitate their nutrition, increase water absorption, and improve soil structure. Although the AMF association is already known in some species of Myrciaria, no report is available on its association in M. dubia. This study presents, for the first time, the symbiotic association between AMF and M. dubia from the INIA San Roque experimental station located i...

The rocoto (Capsicum pubescens Ruiz & Pav.) is a nightshade with a center of origin in Peru, with great gastronomic value. It is affected by diseases that limit its development, spread by infected seeds. The in vitro culture of plant tissues allows the propagation of elite genotypes with the availability of pathogen-free plant material. The objective was to determine the in vitro response of hot pepper in the establishment of seeds and regeneration from terminal buds. Hot pepper seeds were used in different treatments with sodium hypochlorite. Leaves and roots of germinated rocotts were removed in vitro, leaving epicotyl with terminal buds and transferred to culture media with different concentrations of salts and MS vitamins for the in vitro regeneration phase, the organogenic response and oxidation were evaluated. In the establishment of hot pepper seeds, 100% disinfection was obta...

La papa chaucha (Solanum tuberosum L. grupo Phureja) presenta un desarrollo rápido, gran adaptación, genes de resistencia a enfermedades, bajo costo de producción, alto contenido nutricional y calidad culinaria, pero presenta también baja productividad y heterogeneidad. Las herramientas biotecnologías mediante el cultivo de tejidos vegetales in vitro permite la multiplicación homogénea de plántulas de mayor rendimiento, y a la vez conservar germoplasma de gran interés agronómico. Por lo tanto, la presente investigación tuvo como objetivo multiplicar y reducir el crecimiento de papa chaucha en condiciones in vitro. Se emplearon nudos de papa chaucha para la multiplicación y conservación, en la multiplicación se emplearon diferentes concentraciones de BAP y AG3 adicionadas al medio de cultivo MS, mientras que en la reducción se emplearon diferentes porcentajes de manitol en ...

Cocoa (Theobroma cacao L.) is one of the most important economic crops worldwide. The propagation of elite varieties of cocoa has been achieved through somatic embryogenesis, but still one of the main limitations is the low rates of embryo formation, which is a genotype-dependent trait. Manipulation of transcription factors (TFs) such as BABY BOOM (BBM) promotes the transition of cocoa somatic cells from the vegetative to the embryonic state. This work validated the use of clonal cocoa leaves cv. IMC-67 to induce somatic embryogenesis, overcoming their recalcitrant limitation with the help of the introduction of TF-BBM from Arabidopsis thaliana (AtBBM). The vectors were constructed by the Gateway system using the donor vector pENTR/D-TOPO and the expression vector pk7WG2. The overexpression vector pk7WG2:AtBBM was obtained, allowing successful transformation into Agrobacterium tumefacien...

El ají supano o arnaucho (Capsicum chinense Jacq.), es un ecotipo originario del valle de Supe, provincia de Barranca, de gran importancia en la cultura culinaria del norte chico de la región Lima. En la actualidad su área de cultivo se encuentra reducida, siendo su única área de conservación in situ, por lo que es necesario su conservación en condiciones ex situ, como bancos de semillas o in vitro. La alternativa de conservación en condiciones in vitro permitirá obtener plántulas libres de patógenos, su multiplicación masiva en cualquier época del año y conservación para programas de mejoramiento genéticos. Con este contexto, se planteó el objetivo de obtener plántulas completas mediante la regeneración in vitro de arnaucho a partir de yemas apicales. Se establecieron in vitro semillas de arnaucho desinfectando con alcohol (70%) y NaClO (1.5 %) y cultivados en medio M...

El ají supano o arnaucho (Capsicum chinense Jacq.), es un ecotipo originario del valle de Supe, provincia de Barranca, de gran importancia en la cultura culinaria del norte chico de la región Lima. En la actualidad su área de cultivo se encuentra reducida, siendo su única área de conservación in situ, por lo que es necesario su conservación en condiciones ex situ, como bancos de semillas o in vitro. La alternativa de conservación en condiciones in vitro permitirá obtener plántulas libres de patógenos, su multiplicación masiva en cualquier época del año y conservación para programas de mejoramiento genéticos. Con este contexto, se planteó el objetivo de obtener plántulas completas mediante la regeneración in vitro de arnaucho a partir de yemas apicales. Se establecieron in vitro semillas de arnaucho desinfectando con alcohol (70%) y NaClO (1.5 %) y cultivados en medio M...