The use of oil and its derivatives has been intensified by the current high energy demand, but brought with it the increase in environmental accidents such as fuel spills that negatively affect ecosystems. In those environments there are microorganisms capable of surviving such conditions and using petroleum hydrocarbons as a source of carbon and energy; those can be used in bioremediation, with an eco-friendly and cost-effective approach. In this work, bacterial strains with bioremediation potential were isolated and identified in culture media supplemented with oil from an area contaminated from an oil spill in the Peruvian Amazon; also was done out the characterization of the bacterial community   by independent analysis of culture by means of next generation sequencing directed to the 16S rRNA gene. The isolated bacterial strains were identified as Acinetobacter rudis, Ente...

The use of oil and its derivatives has been intensified by the current high energy demand, but brought with it the increase in environmental accidents such as fuel spills that negatively affect ecosystems. In those environments there are microorganisms capable of surviving such conditions and using petroleum hydrocarbons as a source of carbon and energy; those can be used in bioremediation, with an eco-friendly and cost-effective approach. In this work, bacterial strains with bioremediation potential were isolated and identified in culture media supplemented with oil from an area contaminated from an oil spill in the Peruvian Amazon; also was done out the characterization of the bacterial community   by independent analysis of culture by means of next generation sequencing directed to the 16S rRNA gene. The isolated bacterial strains were identified as Acinetobacter rudis, Ente...

The use of oil and its derivatives has been intensified by the current high energy demand, but brought with it the increase in environmental accidents such as fuel spills that negatively affect ecosystems. In those environments there are microorganisms capable of surviving such conditions and using petroleum hydrocarbons as a source of carbon and energy; those can be used in bioremediation, with an eco-friendly and cost-effective approach. In this work, bacterial strains with bioremediation potential were isolated and identified in culture media supplemented with oil from an area contaminated from an oil spill in the Peruvian Amazon; also was done out the characterization of the bacterial community   by independent analysis of culture by means of next generation sequencing directed to the 16S rRNA gene. The isolated bacterial strains were identified as Acinetobacter rudis, Ente...

The use of oil and its derivatives has been intensified by the current high energy demand, but brought with it the increase in environmental accidents such as fuel spills that negatively affect ecosystems. In those environments there are microorganisms capable of surviving such conditions and using petroleum hydrocarbons as a source of carbon and energy; those can be used in bioremediation, with an eco-friendly and cost-effective approach. In this work, bacterial strains with bioremediation potential were isolated and identified in culture media supplemented with oil from an area contaminated from an oil spill in the Peruvian Amazon; also was done out the characterization of the bacterial community   by independent analysis of culture by means of next generation sequencing directed to the 16S rRNA gene. The isolated bacterial strains were identified as Acinetobacter rudis, Ente...

Cocoa (Theobroma cacao L.) is one of the most important economic crops worldwide. The propagation of elite varieties of cocoa has been achieved through somatic embryogenesis, but still one of the main limitations is the low rates of embryo formation, which is a genotype-dependent trait. Manipulation of transcription factors (TFs) such as BABY BOOM (BBM) promotes the transition of cocoa somatic cells from the vegetative to the embryonic state. This work validated the use of clonal cocoa leaves cv. IMC-67 to induce somatic embryogenesis, overcoming their recalcitrant limitation with the help of the introduction of TF-BBM from Arabidopsis thaliana (AtBBM). The vectors were constructed by the Gateway system using the donor vector pENTR/D-TOPO and the expression vector pk7WG2. The overexpression vector pk7WG2:AtBBM was obtained, allowing successful transformation into Agrobacterium tumefacien...