La lixiviación con cianuro es actualmente el proceso principalmente utilizado por la industria minera para extraer oro y plata. El cianuro es un compuesto tóxico para la mayoría de los organismos vivos, sin embargo, muchos microorganismos son capaces de tolerarlo e incluso degradarlo. En el presento trabajo ha sido caracterizada y analizada, por metagenómica dirigida al ADNr, la composición de las comunidades microbianas de suelos y aguas, prístinos o contaminados por residuos de cianuro producto de la actividad minera en la Región La Libertad. Se logró aislar e identificar molecularmente 89 cepas microbianas, siendo los géneros Pseudomonas, Bacillus y Alcaligenes los más abundantes. La capacidad de degradación del cianuro ha sido establecida en base a pruebas in vitro considerando estas cepas, ya sea individualmente o en consorcios. El mejor consorcio constituido por 8 cepas ...

In the present study, bacterial communities in soil and water samples, from artisanal leaching pools with cyanide, were characterized by dependent and independent culture analyzes. For the characterization of the culturable bacterial community, classical techniques of microbiology were used, until obtaining pure strains, which were identified at the molecular level. On the other hand, uncultured bacterial communities were characterized by next-generation sequencing of the 16S rRNA gene. The culturable bacterial community was mainly represented by the genera Pseudomonas, Bacillus and Acinetobacter; while the predominant uncultured communities, in soil samples, were the proteobacteria (12.91%), Firmicutes (11.32%), Actinobacteria (11.25%) and Bacteroidetes (10.16%). On the other hand, in water samples, the edges of Firmicutes (59.16%) and Actinobacteria (38.99%) predominated.

In the present study, bacterial communities in soil and water samples, from artisanal leaching pools with cyanide, were characterized by dependent and independent culture analyzes. For the characterization of the culturable bacterial community, classical techniques of microbiology were used, until obtaining pure strains, which were identified at the molecular level. On the other hand, uncultured bacterial communities were characterized by next-generation sequencing of the 16S rRNA gene. The culturable bacterial community was mainly represented by the genera Pseudomonas, Bacillus and Acinetobacter; while the predominant uncultured communities, in soil samples, were the proteobacteria (12.91%), Firmicutes (11.32%), Actinobacteria (11.25%) and Bacteroidetes (10.16%). On the other hand, in water samples, the edges of Firmicutes (59.16%) and Actinobacteria (38.99%) predominated.

The use of oil and its derivatives has been intensified by the current high energy demand, but brought with it the increase in environmental accidents such as fuel spills that negatively affect ecosystems. In those environments there are microorganisms capable of surviving such conditions and using petroleum hydrocarbons as a source of carbon and energy; those can be used in bioremediation, with an eco-friendly and cost-effective approach. In this work, bacterial strains with bioremediation potential were isolated and identified in culture media supplemented with oil from an area contaminated from an oil spill in the Peruvian Amazon; also was done out the characterization of the bacterial community   by independent analysis of culture by means of next generation sequencing directed to the 16S rRNA gene. The isolated bacterial strains were identified as Acinetobacter rudis, Ente...

The use of oil and its derivatives has been intensified by the current high energy demand, but brought with it the increase in environmental accidents such as fuel spills that negatively affect ecosystems. In those environments there are microorganisms capable of surviving such conditions and using petroleum hydrocarbons as a source of carbon and energy; those can be used in bioremediation, with an eco-friendly and cost-effective approach. In this work, bacterial strains with bioremediation potential were isolated and identified in culture media supplemented with oil from an area contaminated from an oil spill in the Peruvian Amazon; also was done out the characterization of the bacterial community   by independent analysis of culture by means of next generation sequencing directed to the 16S rRNA gene. The isolated bacterial strains were identified as Acinetobacter rudis, Ente...

The use of oil and its derivatives has been intensified by the current high energy demand, but brought with it the increase in environmental accidents such as fuel spills that negatively affect ecosystems. In those environments there are microorganisms capable of surviving such conditions and using petroleum hydrocarbons as a source of carbon and energy; those can be used in bioremediation, with an eco-friendly and cost-effective approach. In this work, bacterial strains with bioremediation potential were isolated and identified in culture media supplemented with oil from an area contaminated from an oil spill in the Peruvian Amazon; also was done out the characterization of the bacterial community   by independent analysis of culture by means of next generation sequencing directed to the 16S rRNA gene. The isolated bacterial strains were identified as Acinetobacter rudis, Ente...

The use of oil and its derivatives has been intensified by the current high energy demand, but brought with it the increase in environmental accidents such as fuel spills that negatively affect ecosystems. In those environments there are microorganisms capable of surviving such conditions and using petroleum hydrocarbons as a source of carbon and energy; those can be used in bioremediation, with an eco-friendly and cost-effective approach. In this work, bacterial strains with bioremediation potential were isolated and identified in culture media supplemented with oil from an area contaminated from an oil spill in the Peruvian Amazon; also was done out the characterization of the bacterial community   by independent analysis of culture by means of next generation sequencing directed to the 16S rRNA gene. The isolated bacterial strains were identified as Acinetobacter rudis, Ente...