El rocoto (Capsicum pubescens Ruiz & Pav.) es una planta herbácea o arbustico, se diferencia de otras especies del género Capsicum por su corola de color púrpura y semillas negras. Se considera al Perú como su centro de origen, donde es muy empleado en la medicina, industria farmacéutica, y sobre todo en la gastronomía. En el presente trabajo se desarrolló una metodología para determinar el efecto de la concentración de 2,4-diclorofenoxiacético (2,4-D) en la inducción de callos in vitro a partir de cotiledones de rocoto cv. Serrano. Se germinaron in vitro semillas de rocoto, de las cuales a la primera semana se escindieron los cotiledones y fueron colocados en los medios de cultivo MS adicionado con 2,4-D. Se evaluó cinco tratamientos con diferentes concentraciones de 2,4-D. Los mejores resultados fueron obtenidos con los tratamientos que se adicionaron 0.75 y 1 mg·L-1 para ...

The rocoto (Capsicum pubescens Ruiz & Pav.) is a nightshade with a center of origin in Peru, with great gastronomic value. It is affected by diseases that limit its development, spread by infected seeds. The in vitro culture of plant tissues allows the propagation of elite genotypes with the availability of pathogen-free plant material. The objective was to determine the in vitro response of hot pepper in the establishment of seeds and regeneration from terminal buds. Hot pepper seeds were used in different treatments with sodium hypochlorite. Leaves and roots of germinated rocotts were removed in vitro, leaving epicotyl with terminal buds and transferred to culture media with different concentrations of salts and MS vitamins for the in vitro regeneration phase, the organogenic response and oxidation were evaluated. In the establishment of hot pepper seeds, 100% disinfection was obta...

The rocoto (Capsicum pubescens Ruiz & Pav.) is a nightshade with a center of origin in Peru, with great gastronomic value. It is affected by diseases that limit its development, spread by infected seeds. The in vitro culture of plant tissues allows the propagation of elite genotypes with the availability of pathogen-free plant material. The objective was to determine the in vitro response of hot pepper in the establishment of seeds and regeneration from terminal buds. Hot pepper seeds were used in different treatments with sodium hypochlorite. Leaves and roots of germinated rocotts were removed in vitro, leaving epicotyl with terminal buds and transferred to culture media with different concentrations of salts and MS vitamins for the in vitro regeneration phase, the organogenic response and oxidation were evaluated. In the establishment of hot pepper seeds, 100% disinfection was obta...