The aim of this study was to identify and serotype isolate strains from vendors’ hands, chopping boards and vending tables of 50 chicken meat stalls in a market in San Juan de Miraflores district, Lima, Peru. The sampling was carried out with sterile swabs on the indicated surfaces. The Stuard medium was used to transport the samples to the laboratory. The enrichment was carried out in trypticase soy broth and the isolation was developed on McConkey agar. Positive lactose colonies compatible with E. coli were identified by standard biochemical tests. Serotyping was performed using the method described by Kauffman to detect somatic (O) and flagellar (H) antigens with specific SERUNAM antisera. Results showed that 42% (63/150) of the samples were identified as E. coli, and 58 viable strains were serotyped, belonging to 40 serotypes, where O6H10 was the most frequent (10.3%, 6/58).  

The aim of this study was to identify and serotype isolate strains from vendors’ hands, chopping boards and vending tables of 50 chicken meat stalls in a market in San Juan de Miraflores district, Lima, Peru. The sampling was carried out with sterile swabs on the indicated surfaces. The Stuard medium was used to transport the samples to the laboratory. The enrichment was carried out in trypticase soy broth and the isolation was developed on McConkey agar. Positive lactose colonies compatible with E. coli were identified by standard biochemical tests. Serotyping was performed using the method described by Kauffman to detect somatic (O) and flagellar (H) antigens with specific SERUNAM antisera. Results showed that 42% (63/150) of the samples were identified as E. coli, and 58 viable strains were serotyped, belonging to 40 serotypes, where O6H10 was the most frequent (10.3%, 6/58).  

En la presente investigación se analiza el desarrollo de las políticas públicas a cargo del Estado peruano en materia de prevención de propagación internacional de enfermedades a propósito de la pandemia provocada por el COVID-19. La estrategia de análisis parte de la revisión sistemática de las disposiciones normativas dada por la Organización Mundial de la Salud -especialmente las dictadas mediante el Reglamento Sanitario Internacional-, la Organización panamericana de la Salud y las entidades del Estado peruano encargadas de desarrollar e implementar las políticas públicas de respuesta de prevención y control de la propagación del virus SARS-CoV-2 (COVID-19). Se obtuvo como resultado que el Estado peruano no diseñó ni implementó las políticas públicas como respuesta de prevención en el caso de propagación internacional de enfermedades, conforme lo había exigido ...

Introducción: Las infecciones orales representan una de las principales complicaciones postoperatorias en cirugías bucales menores, siendo causadas por diversos microorganismos como bacterias, virus y hongos. De estas, las bacterias, principalmente Staphylococcus, Streptococcus y Prevotella, son las más prevalentes. Según datos emitidos por la OMS, 3.500 millones de habitantes en el mundo padecen de enfermedades orales. En el contexto de las cirugías bucales menores, la exodoncia de terceros molares viene a ser los procedimientos más habituales, con una incidencia de infecciones postoperatorias que varía entre 0.4 % y 6 %, incrementándose en pacientes mayores de 36 años. Objetivo general: Determinar la frecuencia de infecciones postoperatorias tras cirugías bucales menores en pacientes atendidos en un Centro Dental Docente, Lima-Perú, 2025. Materiales y métodos: Este estudio ...

La presente investigación tiene por finalidad establecer la relación que existe entre las habilidades socioemocionales y la convivencia en el aula de los estudiantes del Vil ciclo de la educación básica regular en las instituciones educativas públicas del distrito de Breña, 2015. Para cumplir con esta finalidad se ha recurrido al estudio descriptivo, de diseño no experimental, de tipo correlaciona! desarrollado en el enfoque cuantitativo; cuya población está compuesta por 1850 estudiantes del 3o, 4o y 5o grado de educación secundaria, que corresponden al Vil ciclo, de la educación básica regular de las instituciones educativas públicas del distrito de Breña con una muestra de 318 estudiantes determinados mediante un muestreo probabilístico, aleatorio simple. Asimismo, para construir y demostrar la validación de los instrumentos se ha considerado la técnica del juicio de ...

El presente estudio tuvo como objetivo identificar terneros con infección congénita con el virus de la diarrea viral bovina (VDVB), en dos establos lecheros de crianza intensiva ubicados en las irrigaciones de Santa Rita de Siguas (Establo A) y Vitor (Establo B) de la cuenca lechera de Arequipa mediante la detección del VDVB en la sangre de los terneros a través de la prueba de ELISA de captura de antígeno. Con esta finalidad se obtuvieron muestras de sangre con anticoagulante de terneros de los establos A (n = 36) y B (n = 95) poco después de nacer y antes que tomen el calostro. El 0.76% (1/131) con IC mínimo 0.24 y máximo 3.5% de los terneros muestreados resulto ser un ternero con infección congénita o persistentemente infectado (PI) con el VDVB. El ternero PI fue detectado en el establo A indicando que la prevalencia de animales PI en este establo fue de 2.78% (1/36). De los...

Determina los patógenos bacterianos y parasitarios más frecuentes en cuyes de crianza familiar - comercial en tres distritos de la Provincia de Bolognesi, Departamento de Ancash. El estudio se realiza en todas las granjas de cuyes de crianza familiar - comercial de dichos distritos. Muestras de animales con signos compatibles a los agentes en evaluación, son recolectadas en el periodo del estudio. Además, se colectaron muestras de heces de camas de una población representativa. Los factores de riesgo evaluados son: tipo de crianza (productor capacitado y No capacitado), sexo (macho, hembra), raza (mejorado y criollo), grupo etareo (recría y reproductores), y localidad de origen (Aquia, Pacarenca y PamPam). Necropsia y obtención de muestras bacteriológicas, se realiza en 51 animales que muestran signos compatibles con las enfermedades en estudio, la toma de muestras de los órgano...

En la presente investigación se analiza el desarrollo de las políticas públicas a cargo del Estado peruano en materia de prevención de propagación internacional de enfermedades a propósito de la pandemia provocada por el COVID-19. La estrategia de análisis parte de la revisión sistemática de las disposiciones normativas dada por la Organización Mundial de la Salud -especialmente las dictadas mediante el Reglamento Sanitario Internacional-, la Organización panamericana de la Salud y las entidades del Estado peruano encargadas de desarrollar e implementar las políticas públicas de respuesta de prevención y control de la propagación del virus SARS-CoV-2 (COVID-19). Se obtuvo como resultado que el Estado peruano no diseñó ni implementó las políticas públicas como respuesta de prevención en el caso de propagación internacional de enfermedades, conforme lo había exigido ...

En la presente investigación se analiza la respuesta del Estado peruano, desde el punto de vista del diseño y de la implementación de las políticas públicas, a raíz de la ola migratoria de ciudadanos extranjeros, principalmente venezolanos, que en los últimos años han contribuido a incrementar la criminalidad en las principales ciudades del Perú. La estrategia de análisis parte de la revisión sistemática de los reportes en materia de criminalidad a cargo de las instituciones especializadas y de los órganos de administración de justicia que viene enfrentando este fenómeno. Se concluye que el Estado peruano no requiere establecer estrategias específicas contra la criminalidad de los migrantes, sino del diseño e implementación de políticas públicas integrales en materia de seguridad ciudadana para enfrentar con eficacia el incesante incremento de los índices de criminal...

Dermatophytosis is a disease that affects the stratum corneum of the skin, hair and nails in Guinea pigs, causing bad aspect of the carcass, affecting its commercialization and generating economic losses. For the study 189 samples of Guinea pigs with dermatological lesions were collected in intensive breeding farms; the Guinea pigs were analyzed by cytopathology and mycological culture in the Laboratory of Microbiology and Microscopy of Universidad Científica del Sur. The frecuency of dermatophytosis was 18.5 ± 5.5 % by mycological culture and 43 ± 7.1 % by cytopathology; according to the age stratum, the dermatophytosis frecuency was 0 % / 0 % in breeding, 25.6 % / 62 % in rearing, and 4.8 % / 6 % in reproductive Guinea pigs by mycological culture and cytopathology, respectively. About the location of the lessions, a frequency of 0 % / 0 % was found in cages by both techniques, while...

The aim of this study was to determine the frequency of Campylobacter spp in biologically appropriate raw food (BARF) for dogs that are marketed in Lima, Peru. One hundred random samples of BARF food were collected from 10 commercial brands. Samples were collected from different batches. The horizontal method for the detection and enumeration of Campylobacter spp (ISO 10272-1:2017) was performed. Bolton Broth was used as enrichment medium and a CCDA selective medium, followed by a second Karmali selective medium for Campylobacter. Suspicious colonies were reseeded on Blood Agar. Campylobacter spp strains were isolated in 5 + 4.2% (5/100) of the samples.

El objetivo del presente estudio fue determinar la actividad antimicótica in vitro del aceite de romero (Rosmarinus Officinalis) sobre Microsporum canis. Se aisló una cepa de M. canis a partir de muestras clínicas. El aceite de romero se obtuvo por el método de arrastre a vapor con una concentración del 100% de pureza. Mediante el método de difusión en pocillo se enfrentó la cepa de M. canis a ocho concentraciones del aceite de romero (250, 500, 1000, 2000, 5000, 10 000, 50 000 y 100 000 ppm). Los resultados indicaron una adecuada sensibilidad de M. canis a partir de la concentración de 50 000 ppm.

The aim of the present study was to identify bacteria present in the uterine mucosa of alpaca before and after the copula. Samples of uterine mucosa were taken (177 before and 60 after copulation). For the collection of the sample, metallic speculum and protected swabs were used to avoid sample contamination. The samples were conserved and transported to the laboratory in tubes with Stuart transport medium at 4 °C. Samples were cultured on blood agar and MacConkey agar at 37 °C for 24 h and bacterial identification was performed through biochemical tests. A total of 165 bacterial isolates and 29 yeasts in the pre-copula group and 64 bacterial isolates and 6 yeasts in the post-copula group were found. The bacteria most frequently isolated were E. coli (24.9%), Providencia stuartii (16.5%), Shigella sp (16.5%), Hafnia alvei (5.9%), Serratia rubidae (5.9%) and yeasts (14.8%).

The objective of the present study was to determine the presence of Salmonella spp in wild birds that live in the surroundings of a well-managed guinea pig farm. Free-living wild birds were captured (n=185) and eight species were identified: Passer domesticus (63.8%), Volatinia jacarina (16.8%), Troglodytes aedon (2.2%), Pyrocephalus rubinus (1.1%), Molothrus bonariensis (0.5%), Crotophaga sulcirostris (1.1%), Columbina cruziana (12.9%) and Forpus coelestis (1.6%). Samples were taken through cloacal swab. Salmonella spp was isolated in 4.32% (8/185) of the wild birds, but only in Passer domesticus, Volatinia jacarina and Forpus coelestis. Likewise, two enrichment methods were used, obtaining 2.2% (4/185) of samples positive for Salmonella spp with standard enrichment and 3.8% (7/185) with delayed enrichment.

The aim of this study was to identify, serotype and determine the sensitivity profile of Salmonella enterica strains isolated from red-eared slider turtles (Trachemys sp) from two breeding centres in Lima, Peru, through cloacal swabs. The samples were isolated using tetrathionate enrichment broth, specific media such as Salmonella-Shigella agar and xylose lysine deoxycholate agar. The identification was made through biochemical tests. Serotyping through the recognition of somatic (O) and flagellar (H) antigens of the Kauffmann-White system, and the determination of sensitivity using the Kirby Bauer diffusion disk test. The results showed that 6.1% (4/66) of Salmonella enterica isolates were obtained, identifying the serotypes Salmonella Saintpaul and Salmonella Infantis. Antibacterial sensitivity tests indicated differences between strains, showing 100% sensitivity against nalidixic acid...

The aim of this study was to determine antibodies against five serovars of Leptospira spp in guinea pigs of family-commercial breeding in Cajamarca, Peru. Blood samples were collected from 242 apparently healthy guinea pigs destined for consumption on five farms. The sera were processed using the microagglutination test (MAT), considering titers >1/100 as seropositive. Reference strains from the serovars Bratislava, Canicola, Hardjo, Icterohaemorrhagiae and Pomona were used. Seroprevalences of 40.50% (98/242) (95% CI: 34.3-46.7%) were found to antibodies against Leptospira spp. The reactive serovars were Icterohaemorrhagiae (19.01%, 46/242), Canicola (16.53%, 40/242) and Pomona (8.68%, 21/144). There was no seroreactivity to Bratislava and Hardjo serovars. There was no statistical association with sex or farm. Seropositivity indicates exposure and circulation of Leptospira spp, which ...

The aim of this study was to determine the seroprevalence of antibodies against smooth strains of Brucella (B. abortus, B. mellitensis and B. suis) in Junín breed sheep of SAIS Tupac Amaru, Peru. Serum samples (n=926) were analysed with a commercial indirect ELISA kit (IDEXX Brucellosis Serum). Only one sample was positive. The beta-pert stochastic simulation program (@Risk) determined a minimum prevalence of 0.10037% and a maximum of 0.11563%.

The aim of this study was to determine the presence of antibiotics in the liver, kidney and muscle of guinea pigs of intensive breeding in four cities of Peru. For this, 410 carcass samples (liver, kidney and muscle) were evaluated. The samples were taken during the air-drying stage ready for sale and sent to the laboratory at 4 °C. The technique used was the microbiological agar diffusion with the Bacillus subtilis ATCC 6633 strain as the sensitive strain and a commercial enrofloxacin 5 µg disc as a positive control. The plates were incubated at 37 °C for 24 hours and then the diameter of the zones of inhibition of bacterial growth was measured with the Kirby-Bauer ruler. Frequencies of bacterial growth inhibition halos of 28.54 + 4.37% (117/410) were obtained in kidney samples, 27.07 + 4.3% (111/410) in muscle, and 26.59 + 4.28% (109/410) in liver. The study shows a high frequency o...

The axolotl is a caudate amphibian used in numerous biomedical studies for its regenerative capacity. It is currently in the category of critical danger of extinction because of aquatic pollution, indiscriminate hunting and introduction of invasive species into its environment. The objective of the present study was to determine the ranges of haematological and biochemical parameters of captive axolotls in Lima, Perú, so that they can be used as reference values during the clinical evaluation in biomedical laboratories and veterinary clinics of unconventional species. The study was carried out in a private collection of axolotls in the district of San Juan de Miraflores, Lima. In total, 27 axolotls were used, which were chemically restricted to perform the blood collection, establishing reference ranges for 13 haematological and 10 biochemical parameters.

The present study aimed to determine the seroprevalence of antibodies against bovine viral diarrhoea virus (BVDV) in the districts of Chumpi, Coracora and Pullo, Ayacucho region, in March 2018. In total, 460 blood samples were collected from cattle from both sexes and older than four months of age, from rural communities under extensive breeding system. The serum samples were analysed using a commercial indirect ELISA kit. The results showed that 82.56 ± 3.44% (367/460) of the animals presented antibodies against BVDV, with the greatest corrected prevalence in Pullo (89.74 ± 2.75%; 83/96). The high corrected prevalence reported shows a wide distribution of the disease.