This study was conducted to determine the effect of phosphorus solubilizing native bacteria on Zea mays L. “maize”, Lycopersicon esculentum Mill. “tomato” and Phaseolus lunatus L. “lima bean” at the lowest part of the valley Chancay, Mochumi, Lambayeque, as an alternative to the problem of limited phosphorus availability. Bacteria were isolated on SRSM (CaHPO4.2H2O = 5.6 gL-1) agar and they were recognized by a translucent halo. The in vitro solubilization efficiency was determined in batch tank bioreactors with an air flow of 0,8 vvm , 1% of inoculum (108 cell mL -1), at 28 ° C for 5 days. 323 isolations were obtained and their halos diameters ranged from 1 to 11 mm, 79.26% of the isolation kept their solubilization capacity, identifying Enterobacter, Bacillus, Erwinia, Micrococcus, Paenibacillus, Serratia, Pseudomonas, Xanthomonas, Burkholderia, Acinetobacter and Azotobact...

In this work, we determine the polyhydroxyalkanoates (PHA) yield of microorganisms of soil and water from salty lagoons of Morrope, Lambayeque, Peru. Microorganisms were enriched in modified HM1 broth with 15, 20 and 25 g/100 mL of NaCl, incubated at 30 °C in aerobiosis. After observed turbidity or film, aliquots were taken and seeded on HM1 agar obtaining 234 bacterial isolates that by lipophilic Sudan Negro B staining were observed the presence of 93 bacterial isolates with PHA granules, which were cultured in HM2 broth with their respective concentrations of NaCl for 144 hours, quantifying a range of 4-85 cells with granules in five microscopic fields observed, selecting 20 bacterial isolates with the presence of 40-85 cells with granules, then they were taken to fermentation where the optimal incubation time was determined, where the cells are clearly differentiated with PHA granule...

En este trabajo se determinó el rendimiento de polihidroxialcanoatos (PHA) producido por microorganismos aislados de suelo y aguas de las salinas de Morrope, Lambayeque, Perú. La población de microorganismos se enriqueció con caldo HM1, con glucosa (15 gL-1) como fuente de carbono, modificado con 15, 20 y 25 g/100 mL de NaCl, se incubó a 30 °C en aerobiosis hasta observar turbidez o película por el crecimiento se aisló en agar HM1 obteniendo cultivos de microorganismos halófilos. En caldo HM2 con glucosa (30 gL-1), con su respectiva concentración de NaCl se investigó la producción de gránulos de PHA y se determinó el tiempo requerido para observar el mayor número de células con gránulos de PHA. Los 20 cultivos con mayor número de células con gránulos se llevaron a fermentación a caldo HM2, cuantificándose la biomasa y PHA. La naturaleza del polímero y cálculo del ...

The growing of Solanum lycopersicum L. "tomato" is affected by salinity, reducing its water consumption, and affecting negatively plant growth and development. Therefore, it is necessary to seek sustainable cultivation and fertilization options, such as the use of plant growth-promoting rhizobacteria. This research aimed to determine the potential of rhizospheric Actinobacteria isolated from Opuntia sp. "prickly pear" as tomato growth promoting under salinity. The genera of isolated Actinobacteria were identified, the synthesis of 1-aminocyclopropane-1-carboxylic acid deaminase (ACC) was characterized and its effect on the germination of tomato cultivar Río Grande seeds was evaluated. Two tests on non-saline and saline soils were carried out to compare the effect of Streptomyces spp. and Nocardia sp. in plant development in the greenhouse. Several Actinobacteria genera were isolated fro...

Objectives: To determine the potential of Echinopsis pachanoi curly fungi "San Pedro Female", as growth promoters in Zea mays L. "corn" under saline stress. Materials and methods: Descriptive cross-sectional study. The fungi were isolated in dextrose potato agar (PDA) and phenotypically identified. The activity of the enzyme 1- aminocyclopropane carboxylate (ACC) deaminase, the production of natures, solubilization of tricalcium phosphate was investigated and the effect on the development of maize, on a saline soil (CE-8,213 dSm-1), under greenhouse conditions. Results: Ten genera of filamentous fungi were identified predominantly Aspergillus spp. (35.92%), Trichoderma spp. (17,96%) and Penicillium spp. (16,16%). The four fungi of the selected genera Trichoderma and Penicillium produced natures, soubilized phosphate and increased the values of maize development parameters, reaching 100.9...

Crops of Vigna unguiculata L. “cowpea” and Zea mays L. “corn” require chemical fertilizers for proper growth and development; however, its inadequate application contaminates the environment, creating the need to search new alternatives that reduce its impact. Due to this, the objective of this research was to determine the effect of a biofertilizer from fish wastes on the yield of cowpea and corn, and its effect on their rhizospheric microbiota. The physicochemical and biological characteristics of the biofertilizer were determined, and then was applied on the field under a completely randomized block design with six treatments: absolute control, chemical control, biofertilizer at 1%, 1.25%, 1.5% and 1.25% plus chemical fertilizer. The application of biofertilizer at 1, 1.25 and 1.5% increased the height, root length and yield of cowpea; however, the increase percentages were lo...

THE HYDRODYNAMIC CAVITATION PROCESS USING ORIFICE PLATES WAS APPLIED TO REMOVE COLOR AND POLYPHENOLS FROM SUGARCANE MOLASSES ETHANOL VINASSE, WITH A TREATMENT TIME OF 1 H. THE RAW VINASSE WAS DILUTED WITH TAP WATER IN A VOLUME RATIO OF 1:10 IN AN ACIDIC MEDIUM (PH 2). A 2K FACTORIAL ANALYSIS WAS CARRIED OUT TO EVALUATE THE EFFECT OF THE INLET PRESSURE OF 2 AND 3.6 BAR. THE RESULTS INDICATE THAT THE INTERACTION BETWEEN THE INLET PRESSURE AND THE NUMBER OF ORIFICES HAS A SIGNIFICANT EFFECT ON THE COLOR AND POLYPHENOL REMOVAL EFFICIENCY. THE PLATE WITH 9 ORIFICES UNDER THE CONDITION OF AN INLET PRESSURE OF 3.6 BAR WAS MORE EFFICIENT, ACHIEVING A MAXIMUM COLOR REMOVAL OF 32.71% AND POLYPHENOL REMOVAL OF 88.62% DURING A 20 MIN TREATMENT, WITH A CAVITATION YIELD OF 0.00365 MG J-1. THE REQUIREMENT OF A LONGER TREATMENT TIME DID NOT FAVOR THE REMOVAL EFFICIENCY OF COLOR AND POLYPHENOLS. HYDRODYN...

URBAN LIGNOCELLULOSIC BIOMASS (ULB), DERIVED FROM THE MAINTENANCE OF PUBLIC AND PRIVATE GREEN SPACES, SUCH AS PARKS, GARDENS, SPORTS FACILITIES, AND AREAS ALONG ROADS, EMERGES AS A SOLID ALTERNATIVE TO REPLACE FOSSIL SOURCES. THESE RESOURCES STAND OUT DUE TO THEIR ABUNDANCE, LOW COST, AND AVAILABILITY. HYDROTHERMAL CARBONIZATION (HTC) EMERGED AS AN EFFICIENT TECHNOLOGY TO PROCESS THIS BIOMASS THAT HAS A HIGH MOISTURE AND ASH CONTENT, ELIMINATING THE NEED FOR PRIOR DRYING. THIS ARTICLE REVIEWS THE CONVERSION OF ULB TO SOLID BIOFUELS THROUGH THE HTC PROCESS, ADDRESSING THE PHYSICOCHEMICAL AND ENERGETIC PROPERTIES OF ULB AND THE RESULTING HYDROCHAR, AS WELL AS THE HTC PROCESS AND THE INFLUENCE OF THE OPERATING VARIABLES THAT AFFECT THE ENERGETIC QUALITY OF HYDROCHAR. THE CARBONIZATION TEMPERATURE (180 TO 280 °C) HAS A VERY SIGNIFICANT INFLUENCE MORE THAN THE RESIDENCE TIME (0.5 TO 24 H) ON...

Objetivo: determinar la producción de polihidroxialcanoatos producidos por bacteriaspotencialmente fijadoras de nitrógeno aisladas de Asparagus officinalis L. (Asparagaceae). Métodos:Muestras de suelo rizosférico, raíces y tallos previamente desinfectados, se cultivaron en mediossin nitrógeno para aislar bacterias fijadoras rizosféricas y endófitas, respectivamente. En caldofermentación con glicerol como fuente de carbono, se investigó la producción de gránulos dePHA por estas bacterias, coloreándolas con Sudan Negro B después de 24 y 48 horas. Los doscultivos bacterianos en los que se observó el mayor número de células con gránulos de PHA sellevaron a fermentación con glicerol en los medios balanceado y desbalanceado durante 36 horas,determinándose la biomasa. Resultado: El 51,1% de las bacterias fijadoras fueron rizosféricas y el48,9% endófitas. En el 56,67% de la...