1
tesis doctoral
Publicado 2009
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This study was conducted to determine the effect of phosphorus solubilizing native bacteria on Zea mays L. “maize”, Lycopersicon esculentum Mill. “tomato” and Phaseolus lunatus L. “lima bean” at the lowest part of the valley Chancay, Mochumi, Lambayeque, as an alternative to the problem of limited phosphorus availability. Bacteria were isolated on SRSM (CaHPO4.2H2O = 5.6 gL-1) agar and they were recognized by a translucent halo. The in vitro solubilization efficiency was determined in batch tank bioreactors with an air flow of 0,8 vvm , 1% of inoculum (108 cell mL -1), at 28 ° C for 5 days. 323 isolations were obtained and their halos diameters ranged from 1 to 11 mm, 79.26% of the isolation kept their solubilization capacity, identifying Enterobacter, Bacillus, Erwinia, Micrococcus, Paenibacillus, Serratia, Pseudomonas, Xanthomonas, Burkholderia, Acinetobacter and Azotobact...
2
artículo
Publicado 2018
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In this work, we determine the polyhydroxyalkanoates (PHA) yield of microorganisms of soil and water from salty lagoons of Morrope, Lambayeque, Peru. Microorganisms were enriched in modified HM1 broth with 15, 20 and 25 g/100 mL of NaCl, incubated at 30 °C in aerobiosis. After observed turbidity or film, aliquots were taken and seeded on HM1 agar obtaining 234 bacterial isolates that by lipophilic Sudan Negro B staining were observed the presence of 93 bacterial isolates with PHA granules, which were cultured in HM2 broth with their respective concentrations of NaCl for 144 hours, quantifying a range of 4-85 cells with granules in five microscopic fields observed, selecting 20 bacterial isolates with the presence of 40-85 cells with granules, then they were taken to fermentation where the optimal incubation time was determined, where the cells are clearly differentiated with PHA granule...
3
artículo
Publicado 2018
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En este trabajo se determinó el rendimiento de polihidroxialcanoatos (PHA) producido por microorganismos aislados de suelo y aguas de las salinas de Morrope, Lambayeque, Perú. La población de microorganismos se enriqueció con caldo HM1, con glucosa (15 gL-1) como fuente de carbono, modificado con 15, 20 y 25 g/100 mL de NaCl, se incubó a 30 °C en aerobiosis hasta observar turbidez o película por el crecimiento se aisló en agar HM1 obteniendo cultivos de microorganismos halófilos. En caldo HM2 con glucosa (30 gL-1), con su respectiva concentración de NaCl se investigó la producción de gránulos de PHA y se determinó el tiempo requerido para observar el mayor número de células con gránulos de PHA. Los 20 cultivos con mayor número de células con gránulos se llevaron a fermentación a caldo HM2, cuantificándose la biomasa y PHA. La naturaleza del polímero y cálculo del ...
4
artículo
Publicado 2021
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Objectives: To determine the potential of Echinopsis pachanoi curly fungi "San Pedro Female", as growth promoters in Zea mays L. "corn" under saline stress. Materials and methods: Descriptive cross-sectional study. The fungi were isolated in dextrose potato agar (PDA) and phenotypically identified. The activity of the enzyme 1- aminocyclopropane carboxylate (ACC) deaminase, the production of natures, solubilization of tricalcium phosphate was investigated and the effect on the development of maize, on a saline soil (CE-8,213 dSm-1), under greenhouse conditions. Results: Ten genera of filamentous fungi were identified predominantly Aspergillus spp. (35.92%), Trichoderma spp. (17,96%) and Penicillium spp. (16,16%). The four fungi of the selected genera Trichoderma and Penicillium produced natures, soubilized phosphate and increased the values of maize development parameters, reaching 100.9...