La tuberculosis bovina es una enfermedad infectocontagiosa que afecta una amplia gama de hospederos en los que se encuentra la alpaca. El objetivo de este estudio fue evaluar las características inmunológicas de la tuberculosis latente, utilizando como herramienta diagnóstica una técnica de inmunoensayo (ELISA) para medición de la citoquina interferón gamma (INF-γ), secretada por los leucocitos sensibilizados durante un periodo de cultivo de 24 horas con antígenos específicos (ESAT-6 y CFP10) al Mycobacterium tuberculosis complex. Adicionalmente se evaluó la proteína PPD (derivado proteico purificado) que se emplea en la prueba de reacción cutánea, así como también la utilidad de la secuencia de inserción IS6110 del complejo Mycobacterium tuberculosis y una PCR anidado dirigida contra el gen de la proteína MPB70 de Mycobacterium bovis. Se evaluaron un total de 52 alpacas...

The IGRA (Interferon Gamma Release Assays) test is currently the standard specific test for Mycobacterium tuberculosis infection status. However, a positive test cannot distinguish between active tuberculosis disease (ATBD) and latent tuberculosis infection (LTBI). Developing a test with this characteristic is needed. We conducted longitudinal studies to identify a combination of antigen peptides and cytokines to discriminate between ATBD and LTBI. We studied 54 patients with ATBD disease and 51 with LTBI infection. Cell culture supernatant from cells stimulated with overlapping Mycobacterium tuberculosis novel peptides and 40 cytokines/chemokines were analyzed using the Luminex technology. To summarize longitudinal measurements of analyte levels, we calculated the area under the curve (AUC). Our results indicate that in vitro cell stimulation with a novel combination of peptides (Rv0849...

“The IGRA (Interferon Gamma Release Assays) test is currently the standard specific test for Mycobacterium tuberculosis infection status. However, a positive test cannot distinguish between active tuberculosis disease (ATBD) and latent tuberculosis infection (LTBI). Developing a test with this characteristic is needed. We conducted longitudinal studies to identify a combination of antigen peptides and cytokines to discriminate between ATBD and LTBI. We studied 54 patients with ATBD disease and 51 with LTBI infection. Cell culture supernatant from cells stimulated with overlapping Mycobacterium tuberculosis novel peptides and 40 cytokines/chemokines were analyzed using the Luminex technology. To summarize longitudinal measurements of analyte levels, we calculated the area under the curve (AUC). Our results indicate that in vitro cell stimulation with a novel combination of peptides (Rv0...