The prevalence of bovine viral diarrhea virus (BVDV) was determined in 12 dairy herds of the Lima valley. Blood samples were taken in 311 female cattle older than 6 months of age for the detection of antibodies against BVDV by viral neutralization test. The herds did not have history of vaccination against bovine viral diarrhea disease. The prevalence of BVDVwas 56.0 ± 5.5%(174/311) and the titers of antibodies againstBVDV ranged from 2 to >256. Five out of the 12 herds did not have seropositive animals. The results indicated that the BVDV was widely distributed in dairy herds of Lima valley; although viral prevalence is low and some herds are free of the disease.

The seroprevalence of bovine herpes virus type 1 (BHV-1), the causative agent of Infectious Bovine Rhinotracheitis (IBR) was determined in non-vaccinated bovines older than 6 months of age and reared in 12 dairy farms in the valley of Lima, Peru. Blood samples were collected from 395 animals and assayed for antibodies to BHV-1 by using virus neutralization test. Antibodies against BHV-1 were detected in 26 ± 0.47% (143/395) of the animals with titers between 2 and >256. Sixty seven per cent (8/12) of herds had seropositive animals. The higher prevalence was found in herds with more than 300 animals and in herds located in the north and south of Lima, and in those animals older than 2 years. These results confirmed the wide distribution of BHV-1 in the Lima valley, despite of the absence of clinical signs. Since farmers have indicated to have observed respiratory diseases in young ani...

Durine and Glanders are cronic and progressive infectious diseases of horses, donkeys and mules, and human beings in case of glanders. These diseases are caused by a parasite, Trypanosoma equiperdum (T. equiperdum) and a bacteria Burkholderia mallei (B. mallei), respectively. In Peru there are no information about the clinical and serological evidence of durine and glanders. The objective of this study was to detect antibodies against T. equiperdum and B. mallei in serum samples from horses (n=505) of normal appearance, and from different areas of Peru but mainly from Lima by using the complement fixation test. No antibodies against T. equiperdum and B. mallei were detected in the serum samples tested. These results suggest that both diseases are absent or the prevalence are below 1% in the studied Peruvian horses .

The objective of this study was to identify animal carriers of the Hog Cholera virus (HCV) post vaccination in a well-managed pig farm ofLima valley. A total of 166 serum samples were collected from 166piglets between 6 and 7 weeks of age, vaccinated against Hog Cholera 15 days befare sampling. Specific HCV antibodies were detected using a blocking ELISA test. Eighty eight (146/166) of animals reacted positively against HCV; 3% (5/166) and 9% (15/166) of animals were considered suspects and negatives to antibodies, respectively. A second sample was collected 30 days after the first collection from suspect (n=5) and negative (n=15) animals. HCV was detected by direct inmunofluorescence test using cultivated lymphocytes. At the time of the second sampling, 14 out of20 animals stayed at farm. Antibodies were detected in 6 animals and 8 were negative, however 4 ofthe latter were positive to ...

The seroprevalence of Bovine Herpes Virus-1, the etiological agent of Infectious Bovine Rinotracheitis, was evaluated in criollo cattle from the districts of Coracora, Chumpi, Puyusca and Pullo of the Parinacochas Province, Ayacucho. A total of 469 serum samples from 25 herds were tested by virus neutralisation test to detect neutralizing antibodies. The 67 .6 ± 4.2% (317 /469) of the samples had antibodies against BHV-1. The seroprevalence of the virus was similar in the animals of the 4 districts under evaluation. The antibody titers ranged from 2 to >256. Ali sampled herds had seroreactive animals. The study reported the presence of the BHV-1 in criollo cattle of the Parinacochas province, with a higher prevalence than the described in dairy herds in the country.

The objective of this study was to determine the prevalence of the equine herpes virus type 1 (EHV-1 ), the causa ti ve agent of equine rhinopneumonitis, in mature healthy horses of various breeds reared in Lima valley, Peru. For this purpose serum samples (n = 448) were collected during October 2000 till March 2001 for detecting neutralizing antibodies against EHV-1 by virus neutralisation test. The prevalence ofEHV-1 was 44.2 ± 4.6%. The highest prevalence was detected in Standardbred (52.6%) followed by Thoroughbred (47.1 %) and Peruvian horses de Paso (34.8% ). Antibody titres against EHV-1 ranged between 2 to> 256. There was no statistical association between the presence of antibodies against EHV-1, sex, activity and location of the horses. This results indicate that the EHV-1 has wide distribution in horses reared in the Lima valley, Perú.

El objetivo del presente estudio fue determinar la prevalencia del virus de la diarrea viral bovina (VDVB) en bovinos productores de leche bajo crianza intensiva en el valle de Lima. Se colectaronmuestras de sangre de bovinos hembrasmayores a 6meses (n = 311) procedentes de 12 hatos sin antecedentes de vacunación contra la enfermedad de la diarrea viral bovina, para la detección de anticuerposmediante la prueba de neutralización viral. El 56.0 ± 5.5% (174/311) de las muestras presentaron anticuerpos contra el VDVBcon títulos entre 2 a >256. Cinco de los 12 hatosmuestreados no tuvieron animales serorreactores. Los resultados indicaron que el VDVB estaba difundido en el valle de Lima, aunque se encuentran hatos libres de la infección viral o con una prevalencia viral muy baja.

El presente estudio tuvo como objetivo determinar la seroprevalencia del virus herpes bovino tipo 1 (VHB-1), agente causal de la Rinotraqueitis Infecciosa Bovina (RIB), en bovinos mayores de 6 meses de edad, procedentes de 12 hatos lecheros del valle de Lima y sin historia de vacunación. Se tomó muestras de sangre en 395 hembras para la detección de anticuerpos neutralizantes en suero mediante la prueba de neutralización viral. El 36 ± 0.47% (143/395) de los animales presentaron anticuerpos neutralizantes contra el VHB-1 con títulos entre 2 a >256. El 67% (8/12) de los hatos muestreados tuvieron animales seroreactores. Las mayores prevalencias se presentaron en hatos con >300 animales, en hatos ubicados en el norte y sur del valle de Lima y en animales mayores de 2 años de edad. Estos resultados confirman que el VHB-1 está difundido en el valle de Lima a pesar que no se obt...

Durine and Glanders are cronic and progressive infectious diseases of horses, donkeys and mules, and human beings in case of glanders. These diseases are caused by a parasite, Trypanosoma equiperdum (T. equiperdum) and a bacteria Burkholderia mallei (B. mallei), respectively. In Peru there are no information about the clinical and serological evidence of durine and glanders. The objective of this study was to detect antibodies against T. equiperdum and B. mallei in serum samples from horses (n=505) of normal appearance, and from different areas of Peru but mainly from Lima by using the complement fixation test. No antibodies against T. equiperdum and B. mallei were detected in the serum samples tested. These results suggest that both diseases are absent or the prevalence are below 1% in the studied Peruvian horses .

El objetivo del presente estudio fue detectar animales portadores del virus del cólera porcino (VCP) luego de haber sido vacunados contra el cólera porcino (CP) en una granja tecnificada del valle de Lima. Se colectaron muestras de suero de 166 lechones de 7 a 9 semanas de edad y que fueron vacunados contra el Cólera Porcino l5 días antes del muestreo. La detección de anticuerpos contra el VCP se realizó mediante la prueba de ELISA de competición. Se obtuvieron muestras adicionales de los animales que resultaron negativos o dudosos a la prueba de ELISA a los 30 días posteriores al primer muestreo para la detección del VCP mediante la prueba de inmunofluorescencia en linfocitos cultivados El 88% (146/166) de los animales estudiados presentaron anticuerpos contra el VCP mientras que el 3% (5/166) y el 9% (15/166) de los animales presentaron resultados dudosos y negativos a anticue...

El objetivo del presente estudio fue conocer la prevalencia del Virus Herpes Bovino 1 (VHB-1), agente causal de la Rinotraqueitis Infecciosa Bovina, en bovinos criollos de crianza extensiva de los distritos de Coracora, Chumpi, Puyusca y Pullo de la provincia de Parinacochas, Ayacucho. Se analizaron 469 muestras de sueros bovinos procedentes de 25 hatos para la detección de anticuerpos neutralizantes mediante la prueba de neutralización viral. El 67.6 ± 4.2% (317/469) de las muestras presentó anticuerpos neutralizantes con títulos entre 2 a>256. El 100% de los hatos muestreados tuvo animales seroreactores. La prevalencia del virus fue similar en los 4 distritos estudiados. Este estudio reporta la presencia del VHB-1 en bovinos criollos de la provincia de Parinacochas, con una prevalencia superior a lo descrito en bovinos de las principales cuencas lecheras del país.

The objective of this study was to determine the prevalence of the equine herpes virus type 1 (EHV-1 ), the causa ti ve agent of equine rhinopneumonitis, in mature healthy horses of various breeds reared in Lima valley, Peru. For this purpose serum samples (n = 448) were collected during October 2000 till March 2001 for detecting neutralizing antibodies against EHV-1 by virus neutralisation test. The prevalence ofEHV-1 was 44.2 ± 4.6%. The highest prevalence was detected in Standardbred (52.6%) followed by Thoroughbred (47.1 %) and Peruvian horses de Paso (34.8% ). Antibody titres against EHV-1 ranged between 2 to> 256. There was no statistical association between the presence of antibodies against EHV-1, sex, activity and location of the horses. This results indicate that the EHV-1 has wide distribution in horses reared in the Lima valley, Perú.

The prevalence of infectious agents associated with reproductive failures in Zebu and crossbred cattle (n=268), older than six months of age, from the IVITA Research Center herd in Pucallpa, Peru, was determined. Serum samples were collected for the detection of antibodies against Brucella sp., Leptospira serovares: canicola, icterohemorrhagiae, pomona and hardjo, Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus Type 1 (BHV-1) and Neospora caninum by a commercial indirect ELISA, microglutination, viral neutralization and indirect immunofluorescence tests, respectively. No antibodies were detected against Brucella sp., L. pomona and BVDV. The 46.3 ± 6.0% of the animals had antibodies against BHV-1. The highest seroprevalence of BHV-1 (63.2%) was found in cows older than 18 months. Antibody titres against BHV-1 varied from 2 till >256. The 52.2% of the animals had antibodies aga...

El objetivo del presente estudio fue determinar la prevalencia de agentes infecciosos con impacto reproductivo en bovinos de la Estación Experimental del Trópico del Centro de Investigaciones IVITA, en Pucallpa, Perú. Con este fin, se obtuvieron muestras de suero (n=268) de bovinos cruzados y cebú, mayores de 6 meses de edad, para la detección de anticuerpos contra los virus de la diarrea viral bovina (VDVB) y herpes bovino- 1 (VHB-1), las bacterias Brucella sp. y Leptospira serovares: hardjo, pomona, canicola e icterohemorrhagiae y el protozoo Neospora caninum, mediante técnicas de neutraliza-ción viral, ELISA indirecta, microaglutinación e inmunofluorescencia indirecta, respectivamente. No se detectaron anticuerpos contra el VDVB ni Brucella sp. en los animales muestreados. El 46.3 ± 6.0% de los animales presentaron anticuerpos contra el VHB-1, correspondiendo el 63.2% a anima...