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1
artículo
Publicado 2023
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This study aimed to compare the general electrical heart activity, blood pressure, and heart rate when using three anesthetic protocols for ovariohysterectomy in bitches. In total, 33 healthy canines were selected, distributed in 3 groups of 11 animals each, aged between 1 and 3 years, and with a body weight of 8 to 15 kg from shelters in Bucaramanga, Colombia. Three anesthetic protocols in a completely randomized experimental designwere used. Protocol 1: Ketamine (7 mg/kg) + pentobarbital sodium (1 mg/kg); Protocol 2: Ketamine (7 mg/kg) + propofol (3 mg/kg); Protocol 3: Tiletamine + zolazepam (7 mg/kg). The individual outcomes were classified according to the American Society of Anesthesiologists (ASA I). The results showed that parameters evaluated in the surgical phase significantly increased in patients with Protocol 1 (p<0.05). Protocol 2 showed a better performance in the post-s...
2
artículo
Coordinated biodiversity monitoring systems: an multiscalar unificated approach in forest landscapes
Publicado 2023
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The global biodiversity decrease implies the necessity to study the state of the ecosystem, not only in preserved places but also in landscapes with diversified land use and territories currently under use. Considering the national efforts to gather biological data in Peru as in México and several diverse international referents, from the generation, implementation, and analysis of monitoring biodiversity protocols in forest management areas in both countries, a unified biodiversity monitoring protocol for forest use areas was elaborated through a logical and inclusive process. This protocol is capable of generating data in three different territorial approximation levels, and it can be used in several ecological conditions. The different stages of development of all the activities were implemented for a team divided into two sections with complementary functions: a) Situational analysi...
3
artículo
Publicado 2019
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Th e present investigation was carried out in order to evaluate the stability of the anesthetic monitoring of a protocol based on Meloxican, Acepromazina, Propofol, Xilacina and Tramadol in Oryctolagus cuniculus (Linnaeus, 1758). Eight male rabbits of the New Zealand race were subjected to 3 anesthesia protocols with 8 pseudo replicas: 2 treatments and 1 control with a one week interval between each. Th e fi rst protocol (protocol 1) was based on Meloxicam 0.3 mg·kg-1 SC, Acepromazine 0.5 mg·kg-1 IM, Propofol (induction 8mg·kg-1 maintenance 0.5 mg·kg-1 IV), the second protocol (protocol 2) was based on Meloxicam 0.3 mg·kg-1 SC, Acepromazine 0.5 mg·kg-1 IM, Propofol (induction 8 mg·kg-1 maintenance 0.5 mg·kg-1), Tramadol 5 mg·kg-1 IV and control (control protocol) was based on Meloxicam 0.3 mg·kg-1 SC, Acepromazine 0.5 mg·kg-1 IM, Ketamine 30 mg·kg-1 IM, Xylazine 5 mg·kg-1 IM...
4
artículo
Publicado 2017
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The study aimed to determine changes in respiratory and heart rate, pulse, saturation of oxygen, temperature and blood pressure caused by the combination of tramadol, diazepam, xylazine and ketamine during canine ovariohysterectomy for possible use in sterilization campaigns. Ten bitches between 10 months and 6 years of age of different breeds and sizes, clinically healthy, with hemogram and leucogram normal and classified as ASA I and II, were subjected to elective surgery ovariohysterectomy using the same anesthetic protocol. Physiological data was recorded during the six stages of the anesthetic-surgical procedure. Heart rate and pulse were within normal levels. Three patients presented tachypnea during maintenance. Episodes of mild hypoxia were observed. Body temperature showed a gradual decline. All patients presented hypertension during maintenance and the end of the surgery. There...
5
artículo
Publicado 2017
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The study aimed to determine changes in respiratory and heart rate, pulse, saturation of oxygen, temperature and blood pressure caused by the combination of tramadol, diazepam, xylazine and ketamine during canine ovariohysterectomy for possible use in sterilization campaigns. Ten bitches between 10 months and 6 years of age of different breeds and sizes, clinically healthy, with hemogram and leucogram normal and classified as ASA I and II, were subjected to elective surgery ovariohysterectomy using the same anesthetic protocol. Physiological data was recorded during the six stages of the anesthetic-surgical procedure. Heart rate and pulse were within normal levels. Three patients presented tachypnea during maintenance. Episodes of mild hypoxia were observed. Body temperature showed a gradual decline. All patients presented hypertension during maintenance and the end of the surgery. There...
6
artículo
Publicado 2012
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Actualmente es posible determinar el estatus de especies silvestres mediante el análisis de ADN extraído de muestras no invasivas como las heces. Aunque dichos análisis suelen ser dificultosos debido principalmente a la composición y conservación de las heces, se ha demostrado en muchas especies que con el desarrollo de métodos adecuados de extracción es posible maximizar la fiabilidad y eficacia de este procedimiento. Por tanto, el objetivo del presente estudio fue optimizar un método de extracción de ADN de heces de vicuña Vicugna vicugna mensalis, especie clasificada como casi amenazada en el Perú, con la finalidad de obtener ADN de suficiente calidad para análisis moleculares. Se recolectaron 51 muestras de heces durante los meses de agosto y septiembre de 2008 en dos comunidades vicuñeras ubicadas a más de 4600 msnm en los departamentos de Junín y Moquegua. Luego de o...
7
artículo
At present, the use of DNA extracted from non-invasive samples, especially feces, is common practice in the discipline of conservation genetics. Even though DNA analysis is often difficult mainly due to the composition and preservation of feces has been shown in many species with the development of suitable extraction methods is possible to maximize the reliability and effectiveness of such procedures. The objective of this study has been optimization of a protocol for the extraction of DNA from vicuña feces (Vicugna vicugna mensalis), species classified as Near Threatened in Peru, in order to obtain DNA of sufficient quality for molecular analysis. A total of 51 samples were taken from vicuña dung piles in two communities located 4600 meters above sea level in the dry Puna ecosystems of the regions of Junin and Moquegua between August and September 2008. After observing defecation, sa...
8
artículo
Publicado 2019
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Objective: To determine the effectiveness of misoprostol using the International Federation of Gynecology and Obstetrics (FIGO) 2017 protocol for medical management of first trimester abortion. Methods: A cross-sectional study was performed between March and September 2018. Seventy-six patients diagnosed with first trimester spontaneous abortion were managed following the FIGO 2017 protocol for medical management of first trimester abortion. Results: Main indications for inclusion of the 76 patients were missed abortion 57.9%, incomplete abortion 25%, and anembryonic pregnancy 17.1%. Medical induction with misoprostol followed the FIGO 2017 protocol for medical management of first trimester abortion. The most frequent side effect reported was abnormal uterine bleeding associated with pelvic pain (72.3% of patients). Complete uterine evacuation was accomplished in 98.6% of the patients. C...
9
artículo
Publicado 2012
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El objetivo del estudio fue evaluar la respuesta inmune de macroquistes pequeños y grandes de Sarcocystis aucheniae utilizando tres protocolos de inoculación. Para la obtención del antígeno se recolectaron cuellos de alpaca con quistes de S. aucheniae del camal de Huancavelica, Perú. Los quistes colectados fueron clasificados según el tamaño, en macroquistes grandes (>5 mm) y pequeños (1-3 mm), obteniéndose una concentración de proteína antigénica de 3.23 y 2.45 mg/ml, respectivamente. Treinta conejos fueron inmunizados con 0.05 mg/kg de proteína antigénica derivada de cada tamaño de macroquiste. Además, se consideró tres protocolos de inoculación (n = 5 conejos). El protocolo I y II con uno y dos meses de duración, respectivamente, donde las proteínas antigénicas fueron inactivadas con formol, y el protocolo III de 2 meses, con proteína antigénica inactivada co...
10
artículo
The aim of this study was to evaluate the immune response of small and large macrocysts of Sarcocystis auchenie using three protocols of inoculation. Alpaca meat samples with S. aucheniae were obtained from the slaughterhouse of Huancavelica, Peru. Cysts were collected and classified by size in large (>5 mm) and small (1-3 mm), obtaining 3.23 and 2.45 mg/ml respectively of antigenic protein by the Biuret method. Fifteen rabbits were immunized with 0.05 mg/kg of antigenic protein derived from large and small macrocysts. Besides, three protocols of inoculation were considered (n = 5 rabitts). Protocol I and II were of 1 and 3 month length where proteins were inactivated with formalin, and protocol III of 2 months length using heat-inactivated antigen protein. Specific immune response Ag-Ab was detected by double immunodiffusion in agar gel in 100% of rabbits using protocols I and II and...
11
artículo
Publicado 2024
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One of the most used ecotoxicity bioassays is based on the luminescence of Vibrio fischeri, for which commercial kits such as Microtox® and several others are available. However, this assay might not always be suitable for several reasons: Its protocol cannot be easily adapted for particular purposes, and it requires the continuous purchases of the kit and the availability of specialized equipment or at least, a luminometer. As a versatile alternative, this paper describes an optimized and kit-free protocol, referred to as “Macrotox”, which allows a wide range of treatment periods (for example, 1, 3, 7, 12, 24, 30 and 36 h) to follow the effects on the luminescence and proliferation of V. fischeri. While the optimized protocol involves 1-mL treatments, it could be easily adapted to use other volumes and times. Importantly, to evaluate the effects on luminescence, only a digital came...
12
artículo
Publicado 2019
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Th e present investigation was carried out in order to evaluate the stability of the anesthetic monitoring of a protocol based on Meloxican, Acepromazina, Propofol, Xilacina and Tramadol in Oryctolagus cuniculus (Linnaeus, 1758). Eight male rabbits of the New Zealand race were subjected to 3 anesthesia protocols with 8 pseudo replicas: 2 treatments and 1 control with a one week interval between each. Th e fi rst protocol (protocol 1) was based on Meloxicam 0.3 mg·kg-1 SC, Acepromazine 0.5 mg·kg-1 IM, Propofol (induction 8mg·kg-1 maintenance 0.5 mg·kg-1 IV), the second protocol (protocol 2) was based on Meloxicam 0.3 mg·kg-1 SC, Acepromazine 0.5 mg·kg-1 IM, Propofol (induction 8 mg·kg-1 maintenance 0.5 mg·kg-1), Tramadol 5 mg·kg-1 IV and control (control protocol) was based on Meloxicam 0.3 mg·kg-1 SC, Acepromazine 0.5 mg·kg-1 IM, Ketamine 30 mg·kg-1 IM, Xylazine 5 mg·kg-1 IM...
13
artículo
Publicado 2020
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This article describes the design and development of a compact low energy consumption temperature data acquisition system using the I2C protocol with cables up to eight meters long. This system has been used in the implementation of a Peruvian government project aimed at validating the use of Trombe walls in small towns located at high altitude (4000 - 5000 m.a.s.l.). This article explains the construction of the module, the data acquisition subsystem, and presents an overview of the implementation of the developed system.
14
artículo
Publicado 2020
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This article describes the design and development of a compact low energy consumption temperature data acquisition system using the I2C protocol with cables up to eight meters long. This system has been used in the implementation of a Peruvian government project aimed at validating the use of Trombe walls in small towns located at high altitude (4000 - 5000 m.a.s.l.). This article explains the construction of the module, the data acquisition subsystem, and presents an overview of the implementation of the developed system.
15
artículo
Publicado 2019
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Objective: To determine the relationship between the level of knowledge and application of the suctioning protocolof secretions in the Intensive Care Unit at the Guillermo Almenara Irigoyen General Hospital. Methodology: Thetype of study is descriptive, cross correlational. A survey was applied to determine the level of suctioning protocolknowledge and to assess their application by the nurses of the Intensive Care Unit. An observation guide was appliedto a total of 43 nurses. The statistical correlation was performed through the chi square. Results: Of the nurses evaluated both in knowledge and application of the protocol (43) 58.1% have knowledge between good and excellent,while 53.5% have a bad level in the application of the suctioning protocol of secretion. The correlation by chi-squaretest shows a significance of 0.5. Conclusions: The level of knowledge does not correlate with the ...
16
tesis de grado
Publicado 2005
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La Parálisis Cerebral es una “afección del Sistema Nervioso producido antes, durante o después del nacimiento por una lesión o defecto del desarrollo en los centros cerebrales”; debido a las diferentes alteraciones motoras, las personas que la sufren requieren de los cuidados adecuados para satisfacer sus necesidades básicas, siendo los padres los principales responsables de tener el conocimiento suficiente para la atención tan especial que ellos requieren, a través de una guía o protocolo que les ayude; es por eso que surge la necesidad de investigar lo siguiente: “Eficacia de un Protocolo de Apoyo Educativo en el Incremento del Conocimiento en Padres de Familia de Niños con Parálisis Cerebral. Hogar Clínica San Juan de Dios”, tuvo como objetivo general: Determinar la Eficacia de un Protocolo de Apoyo Educativo a Padres de Familia de Niños con Parálisis Cerebral. El...
17
artículo
Publicado 2022
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Introduction: Evidence-based and culturally acceptable educational materials have been shown to be an effective tool in promoting healthy eating. Objective: To design a printed nutritional education material using Intervention Mapping protocol for a Lifestyle Clinic. Methods: Four of the steps of the Intervention Mapping protocol were used: 1) needs assessment, 2 identification of behavioral outcomes, performance objectives, and change objectives, 3) selection of theory-based methods and strategies and 4) program development. Results: The Logical Model PRECEDE was built, the matrix of change objectives based on the determinants of knowledge, identification, availability, and information processing was used as a theoretical model. A 22-page magazine was obtained with content, images, text and writing based on the Intervention Mapping steps. Conclusion: The Intervention Mapping process pro...
18
artículo
Publicado 2016
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El venado cola blanca (Odocoileus virginianus peruvianus) es un rumiante de la familia Cervidae que se maneja como especie cinegética en el sector Sauce Grande del Coto de Caza El Angolo (CCEA), Piura, Perú. El objetivo de este trabajo fue proponer un protocolo sanitario para venados cola blanca del CCEA como un componente esencial en sus planes de manejo y que pueda servir como modelo para otras especies silvestres en áreas naturales protegidas. Para el desarrollo de este protocolo se hizo una revisión bibliográfica sobre la especie y se realizaron cinco visitas al CCEA para el reconocimiento del área y la estandarización de algunos procedimientos, donde se tomaron muestras biológicas del venado cola blanca y de mamíferos domésticos de la zona para análisis microbiológicos.
19
artículo
Publicado 2016
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The white-tailed deer (Odocoileus virginianus peruvianus) is a ruminant of the family Cervidae that is managed as a game species in the Sauce Grande area of Coto de Caza El Angolo (CCEA), Piura, Peru. The aim of this study was to propose a health protocol for the white-tailed deer of CCEA as an essential component of their management work plans as well as to serve as a model for other wildlife in protected natural areas. For the development of this protocol a literature review was done on the species, along with five visits to CCEA for the evaluation of the area and the standardization of some procedures, where biological samples were collected from white-tailed deers and domestic mammals in the area for microbiologic analysis.
20
artículo
The aim of this study was to evaluate two protocols for oestrus synchronization using intravaginal progestogens. In the study, 64 ewes with lambs at foot of 30-40 days old were used. The animals were assigned to two groups: (T1): application of a short synchronization protocol (6 days) with suppression of suckling for 30 hours (n1=32) and (T2): application of a long or conventional protocol (12 days) with suppression of suckling for 54 hours (n2=32). Fixed-time artificial insemination (FTAI) was performed with refrigerated semen through the transcervical route at the end of temporary weaning. The pregnancy rate without heat return at 16-17 days post-FTAI, pregnancy rate at 41 days by ultrasound, pregnancy rate at 90 days by balloting and type of delivery were determined. The rate of non-return to oestrus at 15-17 days post-FTAI was 81.25% for T1 and 87.50% for T2 (p<0.05). Likewise, t...
