Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas

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The aim of this study was to compare the techniques of indirect immunofluorescence (IFAT) and Enzimo inmunoensayo (ELISA) techniques for the diagnosis of Neospora caninum Dubey, Carpenter, Speer, Topper & Uggla, 1988 in alpacas from the highlands of Peru. Sensitivity, specificity, positive p...

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Detalles Bibliográficos
Autores: Serrano-Martínez, Enrique, Hinostroza Meza, Clarisa Elizabeth, Quispe Huacho, Marco, Leguia Puente, Guillermo, Burga Cisterna, Cesar
Formato: artículo
Fecha de Publicación:2023
Institución:Universidad Ricardo Palma
Repositorio:Revistas - Universidad Ricardo Palma
Lenguaje:español
OAI Identifier:oai:oai.revistas.urp.edu.pe:article/5630
Enlace del recurso:http://revistas.urp.edu.pe/index.php/Biotempo/article/view/5630
Nivel de acceso:acceso abierto
Materia:alpaca
concordancia
IFI
Neosporosis
sensibilidad
especificidad
concordance
IFAT
sensitivity
specificity
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spelling Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas Comparación de inmunofluorescencia indirecta y ELISA para el diagnóstico de Neospora caninum en alpacasSerrano-Martínez, EnriqueHinostroza Meza, Clarisa Elizabeth Quispe Huacho, MarcoLeguia Puente, GuillermoBurga Cisterna, CesaralpacaconcordanciaIFINeosporosissensibilidadespecificidadalpacaconcordanceIFATNeosporosissensitivityspecificityThe aim of this study was to compare the techniques of indirect immunofluorescence (IFAT) and Enzimo inmunoensayo (ELISA) techniques for the diagnosis of Neospora caninum Dubey, Carpenter, Speer, Topper & Uggla, 1988 in alpacas from the highlands of Peru. Sensitivity, specificity, positive predictive value, negative predictive value and concordance of IFAT were evaluated using a commercial competitive ELISA kit (cELISA - VMRD) as a reference. 1540 sera samples were collected from alpacas naturally exposed to N. caninum. The results showed good sensitivity (92.95%), high specificity (99.75%), positive predictive value of 92.90% and a negative predictive value of 99.04%. The concordance between both diagnostic techniques (IFAT and ELISA) turned out to be almost perfect (k = 0.98). It is concluded that the IFAT test has a high efficiency for the diagnosis of N. caninum in alpacas.El objetivo del estudio fue comparar las técnicas de inmunofluorescencia indirecta (IFI) y Enzimo inmunoensayo (ELISA) para el diagnóstico de Neospora caninum Dubey, Carpenter, Speer, Topper y Uggla, 1988 en alpacas de la sierra del Perú. Se evaluó la sensibilidad, especificidad, valor predictivo positivo, valor predictivo negativo y concordancia de IFI usando de referencia un kit comercial de ELISA competitivo (cELISA - VMRD). Se obtuvieron 1540 muestras de sueros de alpacas expuestas naturalmente a N. caninum. Los resultados muestran una buena sensibilidad (92,95%), alta especificidad (99,75%), valor predictivo positivo de 92,90% y un valor predictivo negativo de 99,04%. La concordancia entre ambas técnicas diagnósticas (IFI y ELISA) resultó ser casi perfecta (k=0,98). Se concluye que la prueba de IFI tiene un alto desempeño para el diagnóstico de N. caninum en alpacas.Facultad de Ciencias Biológicas, Universidad Ricardo Palma2023-05-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTextTextoapplication/pdftext/htmlhttp://revistas.urp.edu.pe/index.php/Biotempo/article/view/563010.31381/biotempo.v20i1.5630Biotempo; Vol. 20 Núm. 1 (2023): Biotempo; 79-842519-56971992-215910.31381/biotempo.v20i1reponame:Revistas - Universidad Ricardo Palmainstname:Universidad Ricardo Palmainstacron:URPspahttp://revistas.urp.edu.pe/index.php/Biotempo/article/view/5630/7940http://revistas.urp.edu.pe/index.php/Biotempo/article/view/5630/8435NoneNingunoDerechos de autor 2023 Biotempoinfo:eu-repo/semantics/openAccessoai:oai.revistas.urp.edu.pe:article/56302023-07-10T15:34:17Z
dc.title.none.fl_str_mv Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas
Comparación de inmunofluorescencia indirecta y ELISA para el diagnóstico de Neospora caninum en alpacas
title Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas
spellingShingle Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas
Serrano-Martínez, Enrique
alpaca
concordancia
IFI
Neosporosis
sensibilidad
especificidad
alpaca
concordance
IFAT
Neosporosis
sensitivity
specificity
title_short Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas
title_full Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas
title_fullStr Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas
title_full_unstemmed Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas
title_sort Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas
dc.creator.none.fl_str_mv Serrano-Martínez, Enrique
Hinostroza Meza, Clarisa Elizabeth
Quispe Huacho, Marco
Leguia Puente, Guillermo
Burga Cisterna, Cesar
author Serrano-Martínez, Enrique
author_facet Serrano-Martínez, Enrique
Hinostroza Meza, Clarisa Elizabeth
Quispe Huacho, Marco
Leguia Puente, Guillermo
Burga Cisterna, Cesar
author_role author
author2 Hinostroza Meza, Clarisa Elizabeth
Quispe Huacho, Marco
Leguia Puente, Guillermo
Burga Cisterna, Cesar
author2_role author
author
author
author
dc.subject.none.fl_str_mv alpaca
concordancia
IFI
Neosporosis
sensibilidad
especificidad
alpaca
concordance
IFAT
Neosporosis
sensitivity
specificity
topic alpaca
concordancia
IFI
Neosporosis
sensibilidad
especificidad
alpaca
concordance
IFAT
Neosporosis
sensitivity
specificity
description The aim of this study was to compare the techniques of indirect immunofluorescence (IFAT) and Enzimo inmunoensayo (ELISA) techniques for the diagnosis of Neospora caninum Dubey, Carpenter, Speer, Topper & Uggla, 1988 in alpacas from the highlands of Peru. Sensitivity, specificity, positive predictive value, negative predictive value and concordance of IFAT were evaluated using a commercial competitive ELISA kit (cELISA - VMRD) as a reference. 1540 sera samples were collected from alpacas naturally exposed to N. caninum. The results showed good sensitivity (92.95%), high specificity (99.75%), positive predictive value of 92.90% and a negative predictive value of 99.04%. The concordance between both diagnostic techniques (IFAT and ELISA) turned out to be almost perfect (k = 0.98). It is concluded that the IFAT test has a high efficiency for the diagnosis of N. caninum in alpacas.
publishDate 2023
dc.date.none.fl_str_mv 2023-05-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Text
Texto
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://revistas.urp.edu.pe/index.php/Biotempo/article/view/5630
10.31381/biotempo.v20i1.5630
url http://revistas.urp.edu.pe/index.php/Biotempo/article/view/5630
identifier_str_mv 10.31381/biotempo.v20i1.5630
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://revistas.urp.edu.pe/index.php/Biotempo/article/view/5630/7940
http://revistas.urp.edu.pe/index.php/Biotempo/article/view/5630/8435
dc.rights.none.fl_str_mv Derechos de autor 2023 Biotempo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2023 Biotempo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.coverage.none.fl_str_mv None
Ninguno
dc.publisher.none.fl_str_mv Facultad de Ciencias Biológicas, Universidad Ricardo Palma
publisher.none.fl_str_mv Facultad de Ciencias Biológicas, Universidad Ricardo Palma
dc.source.none.fl_str_mv Biotempo; Vol. 20 Núm. 1 (2023): Biotempo; 79-84
2519-5697
1992-2159
10.31381/biotempo.v20i1
reponame:Revistas - Universidad Ricardo Palma
instname:Universidad Ricardo Palma
instacron:URP
instname_str Universidad Ricardo Palma
instacron_str URP
institution URP
reponame_str Revistas - Universidad Ricardo Palma
collection Revistas - Universidad Ricardo Palma
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.888049
Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas
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