Comparison of indirect immunofluorescence and ELISA for the diagnosis of Neospora caninum in alpacas

• Autores: Serrano-Martínez, Enrique, Hinostroza Meza, Clarisa Elizabeth, Quispe Huacho, Marco, Leguia Puente, Guillermo, Burga Cisterna, Cesar
• Formato: artículo
• Fecha de Publicación: 2023
• Institución: Universidad Ricardo Palma
• Repositorio: Revistas - Universidad Ricardo Palma
• Lenguaje: español
• OAI Identifier: oai:oai.revistas.urp.edu.pe:article/5630
• Enlace del recurso: http://revistas.urp.edu.pe/index.php/Biotempo/article/view/5630
• Nivel de acceso: acceso abierto
• Materia: alpaca; concordancia; IFI; Neosporosis; sensibilidad; especificidad; concordance; IFAT; sensitivity; specificity

The aim of this study was to compare the techniques of indirect immunofluorescence (IFAT) and Enzimo inmunoensayo (ELISA) techniques for the diagnosis of Neospora caninum Dubey, Carpenter, Speer, Topper & Uggla, 1988 in alpacas from the highlands of Peru. Sensitivity, specificity, positive predictive value, negative predictive value and concordance of IFAT were evaluated using a commercial competitive ELISA kit (cELISA - VMRD) as a reference. 1540 sera samples were collected from alpacas naturally exposed to N. caninum. The results showed good sensitivity (92.95%), high specificity (99.75%), positive predictive value of 92.90% and a negative predictive value of 99.04%. The concordance between both diagnostic techniques (IFAT and ELISA) turned out to be almost perfect (k = 0.98). It is concluded that the IFAT test has a high efficiency for the diagnosis of N. caninum in alpacas.