La fasciolosis es una de las parasitosis hepáticas más importantes del país. Actualmente, las principales limitaciones del control de esta enfermedad son el correcto diagnóstico y la progresiva resistencia al triclabendazol, que es el fármaco recomendado para su tratamiento. Por tal motivo, el presente trabajo propone i) desarrollar y evaluar la precisión diagnóstica de LAMP (Loop-Mediated isothermal amplification) aplicando una optimización en la extracción de ADN en heces de bovinos y ii) evaluar la eficacia fasciolicida in vitro de los extractos de ajo. Investigación 1: Estudio donde se desarrolla una nueva prueba LAMP optimizando la extracción de ADN evaluando su precisión diagnóstica. Se optimizó la técnica de extracción de ADN para F. hepatica combinando la técnica coprológica de sedimentación (concentración de huevos de F. hepatica) y la técnica de lisis con p...

Evalúa la expresión génica de las citoquinas IL-4, IL-5, IL-6, IL-10, TGF- y el factor transcripcional GATA3 en la mucosa intestinal de crías de alpacas clínicamente sanas de 2 a 47 días de edad. Para lo cual se formaron grupos etarios conformados por alpacas de 2 a 8 días (grupo 1), 10 a 21 días (grupo 2) y 26 a 47 días de edad (grupo 3) sin importar sexo, raza y desprovistos de signos clínicos de enfermedad. Se tomó la porción media del yeyuno de cada animal de las cuales se extrajeron el ARN total y se realizó la técnica RT-PCR en tiempo real con cebadores específicos para los genes en estudio. La expresión de genes fue determinado por el método comparativo 2-ΔΔ Ct usando como gen endógeno a Gliceraldehído-3-fosfato deshidrogenasa (GAPDH) y como calibrador fetos de 11 meses. La expresión de los genes de las citoquinas y el factor de transcripción se caracteri...

The aim of this study was to identify the behaviour of canine leptospirosis and its relationship with climatic seasons in the city of Lima, Peru. A retrospective study was designed analysing the results of canine sera that were evaluated with the micro-agglutination test (MAT) and its association with climatic data obtained from the National Meteorology and Hydrology System of Peru (SENAMHI) between 2014 and 2017. A generalized linear model was used to evaluate the association between positive cases to canine leptospirosis and the seasons, together with the adjustment of risk factors. The probability of presenting canine leptospirosis was higher in the spring-summer seasons compared to the autumn-winter season (p<0.05). Dogs diagnosed as positive were exposed to an air temperature of 20.6 °C and a relative humidity of 83.1%. Age, sex and place of origin showed no statistical associat...

The aim of this study was to identify the behaviour of canine leptospirosis and its relationship with climatic seasons in the city of Lima, Peru. A retrospective study was designed analysing the results of canine sera that were evaluated with the micro-agglutination test (MAT) and its association with climatic data obtained from the National Meteorology and Hydrology System of Peru (SENAMHI) between 2014 and 2017. A generalized linear model was used to evaluate the association between positive cases to canine leptospirosis and the seasons, together with the adjustment of risk factors. The probability of presenting canine leptospirosis was higher in the spring-summer seasons compared to the autumn-winter season (p<0.05). Dogs diagnosed as positive were exposed to an air temperature of 20.6 °C and a relative humidity of 83.1%. Age, sex and place of origin showed no statistical associat...

The aim of this study was to compare the techniques of indirect immunofluorescence (IFAT) and Enzimo inmunoensayo (ELISA) techniques for the diagnosis of Neospora caninum Dubey, Carpenter, Speer, Topper & Uggla, 1988 in alpacas from the highlands of Peru. Sensitivity, specificity, positive predictive value, negative predictive value and concordance of IFAT were evaluated using a commercial competitive ELISA kit (cELISA - VMRD) as a reference. 1540 sera samples were collected from alpacas naturally exposed to N. caninum. The results showed good sensitivity (92.95%), high specificity (99.75%), positive predictive value of 92.90% and a negative predictive value of 99.04%. The concordance between both diagnostic techniques (IFAT and ELISA) turned out to be almost perfect (k = 0.98). It is concluded that the IFAT test has a high efficiency for the diagnosis of N. caninum in alpacas.

This study aimed to determine the prevalence and risk factors associated with bovine fasciolosis in peasant communities of the Huancabamba province (Piura, Peru). In total, 360 samples of bovine faeces were collected in the districts of Huancabamba, Sondor, Sondorillo and Carmen de la Frontera. The stool samples were analysed using the Dennis sedimentation technique. Association of the presence of Fasciola hepatica eggs in the stool with the, sex and district of origin of the animals was evaluated. The results showed that 42.5% (95% CI: 37.3-47.8%) of animals were infected with F. hepatica. The highest probability of contracting fasciolosis in cattle was in the age group of 13-18 months (PR: 2.56; 95% CI: 1.51-4.28) and from the Sondorillo district (PR: 1.41; 95% CI: 1.04-1.94); Furthermore, being male was considered a protection factor (PR: 0.69; 95% CI: 0.53-0.90). The study showed a h...

This study aimed to determine the prevalence and risk factors associated with bovine fasciolosis in peasant communities of the Huancabamba province (Piura, Peru). In total, 360 samples of bovine faeces were collected in the districts of Huancabamba, Sondor, Sondorillo and Carmen de la Frontera. The stool samples were analysed using the Dennis sedimentation technique. Association of the presence of Fasciola hepatica eggs in the stool with the, sex and district of origin of the animals was evaluated. The results showed that 42.5% (95% CI: 37.3-47.8%) of animals were infected with F. hepatica. The highest probability of contracting fasciolosis in cattle was in the age group of 13-18 months (PR: 2.56; 95% CI: 1.51-4.28) and from the Sondorillo district (PR: 1.41; 95% CI: 1.04-1.94); Furthermore, being male was considered a protection factor (PR: 0.69; 95% CI: 0.53-0.90). The study showed a h...