Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT

Descripción del Articulo

The objective of this study was to evaluate the prevalence of antibodies against Neospora caninum in dairy cattle farms of the department of Lima, Peru, and the conconrdance between two diagnostic techniques used for the detection of antibodies in serum: enzyme-linked immunosorbent assay (ELISA) and...

Descripción completa

Detalles Bibliográficos
Autores: Serrano-Martínez, Enrique, Evaristo R., Roberto, Quispe H., Marco, Hinostroza M., Elizabeth
Formato: artículo
Fecha de Publicación:2018
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/14757
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14757
Nivel de acceso:acceso abierto
Materia:neosporosis
producción láctea
epidemiologia
IFI
ELISA
milk production
epidemiology
IFAT
id REVUNMSM_8a86ca7a22915e1fffda9de806182d56
oai_identifier_str oai:ojs.csi.unmsm:article/14757
network_acronym_str REVUNMSM
network_name_str Revistas - Universidad Nacional Mayor de San Marcos
repository_id_str
spelling Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFATSeroprevalencia de Neospora caninum en bovinos de Lima y comparación entre ELISA e IFISerrano-Martínez, EnriqueEvaristo R., RobertoQuispe H., MarcoHinostroza M., Elizabethneosporosisproducción lácteaepidemiologiaIFIELISAneosporosismilk productionepidemiologyIFATELISAThe objective of this study was to evaluate the prevalence of antibodies against Neospora caninum in dairy cattle farms of the department of Lima, Peru, and the conconrdance between two diagnostic techniques used for the detection of antibodies in serum: enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT). The study was conducted between June 2015 and December 2016 in serum samples of 3407 dairy cattle from 101 farms in Lima (Barranca, Huaura, Huaral, Canta, Lima, Huarochirí, Cañete and Yauyos). The prevalence of infection to N. caninum was 31.0% (1023/3407) by ELISA and 29.9% (1018/3407) by IFAT. The concordance between both diagnostic techniques was good (K=0.98) and statistically significant (p<0.001). Likewise, 69 of the 101 farms were positive (68.3%). The results showed that cattle from Lima dairy farms have moderate individual prevalence and high intra-herd prevalence against N. caninum.El objetivo del estudio fue evaluar la prevalencia de anticuerpos frente a Neospora caninum en bovinos de establos de la cuenca lechera del departamento de Lima, Perú, y la concordancia entre dos técnicas diagnósticas empleadas para la detección de anticuerpos en suero: ensayo de inmunoabsorción ligado a enzimas (ELISA) e inmunofluorescencia indirecta (IFI). El estudio se realizó entre junio de 2015 y diciembre de 2016 en muestras de suero de 3407 bovinos lecheros provenientes de 101 establos de Lima (Barranca, Huaura, Huaral, Canta, Lima, Huarochirí, Cañete y Yauyos). La prevalencia de la infección a N. caninum fue de 31.0% (1023/3407) obtenida por ELISA y 29.9% (1018/3407) por IFI. La concordancia entre ambas técnicas diagnósticas fue buena (K=0.98) y estadísticamente significativa (p<0.001). Asimismo, 69 de los 101 establos resultaron positivos (68.3%). Los resultados demostraron que los bovinos de establos lecheros de Lima tienen prevalencia individual moderada y alta prevalencia intra-rebaño frente a N. caninum.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2018-09-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1475710.15381/rivep.v29i3.14757Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 3 (2018); 916-922Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 3 (2018); 916-9221682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14757/13088https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14757/13899Derechos de autor 2018 Enrique Serrano-Martínez, Roberto Evaristo R., Marco Quispe H., Elizabeth Hinostroza M.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/147572018-12-21T09:58:01Z
dc.title.none.fl_str_mv Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT
Seroprevalencia de Neospora caninum en bovinos de Lima y comparación entre ELISA e IFI
title Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT
spellingShingle Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT
Serrano-Martínez, Enrique
neosporosis
producción láctea
epidemiologia
IFI
ELISA
neosporosis
milk production
epidemiology
IFAT
ELISA
title_short Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT
title_full Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT
title_fullStr Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT
title_full_unstemmed Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT
title_sort Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT
dc.creator.none.fl_str_mv Serrano-Martínez, Enrique
Evaristo R., Roberto
Quispe H., Marco
Hinostroza M., Elizabeth
author Serrano-Martínez, Enrique
author_facet Serrano-Martínez, Enrique
Evaristo R., Roberto
Quispe H., Marco
Hinostroza M., Elizabeth
author_role author
author2 Evaristo R., Roberto
Quispe H., Marco
Hinostroza M., Elizabeth
author2_role author
author
author
dc.subject.none.fl_str_mv neosporosis
producción láctea
epidemiologia
IFI
ELISA
neosporosis
milk production
epidemiology
IFAT
ELISA
topic neosporosis
producción láctea
epidemiologia
IFI
ELISA
neosporosis
milk production
epidemiology
IFAT
ELISA
description The objective of this study was to evaluate the prevalence of antibodies against Neospora caninum in dairy cattle farms of the department of Lima, Peru, and the conconrdance between two diagnostic techniques used for the detection of antibodies in serum: enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT). The study was conducted between June 2015 and December 2016 in serum samples of 3407 dairy cattle from 101 farms in Lima (Barranca, Huaura, Huaral, Canta, Lima, Huarochirí, Cañete and Yauyos). The prevalence of infection to N. caninum was 31.0% (1023/3407) by ELISA and 29.9% (1018/3407) by IFAT. The concordance between both diagnostic techniques was good (K=0.98) and statistically significant (p<0.001). Likewise, 69 of the 101 farms were positive (68.3%). The results showed that cattle from Lima dairy farms have moderate individual prevalence and high intra-herd prevalence against N. caninum.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14757
10.15381/rivep.v29i3.14757
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14757
identifier_str_mv 10.15381/rivep.v29i3.14757
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14757/13088
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14757/13899
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 3 (2018); 916-922
Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 3 (2018); 916-922
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238229537980416
score 13.888049
Seroprevalence Neospora caninum in bovines of Lima and comparison between ELISA and IFAT
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

Ejemplares Similares