Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)

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The aim of the study was to determine the relative levels of expression of messenger RNAs of RORγt and IL-17 in intestinal mucosa of clinically healthy newborn alpacas. Three age groups were formed: 2 to 7 days (group 1, n=5), 8 to 20 days (group 2, n=6) and 26 to 47 days (group 3, n=6), regardless...

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Detalles Bibliográficos
Autores: Murga A., Gustavo, Manchego S., Alberto, Castro S., Gina, Pezo C., Danilo, Sandoval C., Nieves
Formato: artículo
Fecha de Publicación:2018
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/14831
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14831
Nivel de acceso:acceso abierto
Materia:RORγt
interleukin 17
RT-PCR
relative quantification
intestine
alpaca
interleucina 17
cuantificación relativa
intestino
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spelling Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)Expresión y cuantificación de RORγt e IL-17 en mucosa intestinal de crías de alpacas (Vicugna pacos)Murga A., GustavoManchego S., AlbertoCastro S., GinaPezo C., DaniloSandoval C., NievesRORγtinterleukin 17RT-PCRrelative quantificationintestinealpacaRORγtinterleucina 17RT-PCRcuantificación relativaintestinoalpacaThe aim of the study was to determine the relative levels of expression of messenger RNAs of RORγt and IL-17 in intestinal mucosa of clinically healthy newborn alpacas. Three age groups were formed: 2 to 7 days (group 1, n=5), 8 to 20 days (group 2, n=6) and 26 to 47 days (group 3, n=6), regardless sex or breed. Samples were taken from the middle segment of the jejunum. The total RNA was extracted, and the RT-PCR technique was used with specific primers. The relative expression of mRNA of RORγt and IL-17 was determined by the 2-ΔΔCt method using two neonatal pups as calibrators and using GAPDH as an endogenous control. The kinetics of expression of RORγt showed a constant trend during the first and third week of life, reaching its maximum expression in animals from 2 to 7 days of age, unlike IL-17 whose expression was progressive with age, evidencing a high stimulation and expression from the first days of age.El objetivo del estudio fue determinar los niveles relativos de expresión de ARN mensajeros de RORγt e IL-17 en mucosa intestinal de crías de alpacas clínicamente sanas. Se formaron tres grupos etarios conformados por alpacas de 2 a 7 días (grupo 1, n=5), 8 a 20 días (grupo 2, n=6) y 26 a 47 días (grupo 3, n=6), sin distinción de sexo o raza. Se tomaron muestras del segmento medio del yeyuno, se extrajo el ARN total y se empleó la técnica RT-PCR con cebadores. La expresión relativa de ARNm de RORγt e IL-17 fue determinada por el método 2-∆∆Ct usando como calibradores dos crías neonatas y empleándose como control endógeno a GAPDH. La cinética de expresión de RORγt mostró una tendencia constante durante la primera y tercera semana de vida, alcanzando su máxima expresión en los animales de 2 a 7 días de edad, a diferencia de IL-17 cuya expresión fue progresiva con la edad, evidenciándose una elevada estimulación y expresión desde los primeros días de edad.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2018-09-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1483110.15381/rivep.v29i3.14831Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 3 (2018); 1009-1017Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 3 (2018); 1009-10171682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14831/13100https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14831/13910Derechos de autor 2018 Gustavo Murga A., Alberto Manchego S., Gina Castro S., Danilo Pezo C., Nieves Sandoval C.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/148312018-12-21T09:58:01Z
dc.title.none.fl_str_mv Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
Expresión y cuantificación de RORγt e IL-17 en mucosa intestinal de crías de alpacas (Vicugna pacos)
title Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
spellingShingle Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
Murga A., Gustavo
RORγt
interleukin 17
RT-PCR
relative quantification
intestine
alpaca
RORγt
interleucina 17
RT-PCR
cuantificación relativa
intestino
alpaca
title_short Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
title_full Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
title_fullStr Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
title_full_unstemmed Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
title_sort Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
dc.creator.none.fl_str_mv Murga A., Gustavo
Manchego S., Alberto
Castro S., Gina
Pezo C., Danilo
Sandoval C., Nieves
author Murga A., Gustavo
author_facet Murga A., Gustavo
Manchego S., Alberto
Castro S., Gina
Pezo C., Danilo
Sandoval C., Nieves
author_role author
author2 Manchego S., Alberto
Castro S., Gina
Pezo C., Danilo
Sandoval C., Nieves
author2_role author
author
author
author
dc.subject.none.fl_str_mv RORγt
interleukin 17
RT-PCR
relative quantification
intestine
alpaca
RORγt
interleucina 17
RT-PCR
cuantificación relativa
intestino
alpaca
topic RORγt
interleukin 17
RT-PCR
relative quantification
intestine
alpaca
RORγt
interleucina 17
RT-PCR
cuantificación relativa
intestino
alpaca
description The aim of the study was to determine the relative levels of expression of messenger RNAs of RORγt and IL-17 in intestinal mucosa of clinically healthy newborn alpacas. Three age groups were formed: 2 to 7 days (group 1, n=5), 8 to 20 days (group 2, n=6) and 26 to 47 days (group 3, n=6), regardless sex or breed. Samples were taken from the middle segment of the jejunum. The total RNA was extracted, and the RT-PCR technique was used with specific primers. The relative expression of mRNA of RORγt and IL-17 was determined by the 2-ΔΔCt method using two neonatal pups as calibrators and using GAPDH as an endogenous control. The kinetics of expression of RORγt showed a constant trend during the first and third week of life, reaching its maximum expression in animals from 2 to 7 days of age, unlike IL-17 whose expression was progressive with age, evidencing a high stimulation and expression from the first days of age.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14831
10.15381/rivep.v29i3.14831
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14831
identifier_str_mv 10.15381/rivep.v29i3.14831
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14831/13100
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14831/13910
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 3 (2018); 1009-1017
Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 3 (2018); 1009-1017
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.932913
Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
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