The aim of the study was to determine the relative levels of expression of messenger RNAs of RORγt and IL-17 in intestinal mucosa of clinically healthy newborn alpacas. Three age groups were formed: 2 to 7 days (group 1, n=5), 8 to 20 days (group 2, n=6) and 26 to 47 days (group 3, n=6), regardless sex or breed. Samples were taken from the middle segment of the jejunum. The total RNA was extracted, and the RT-PCR technique was used with specific primers. The relative expression of mRNA of RORγt and IL-17 was determined by the 2-ΔΔCt method using two neonatal pups as calibrators and using GAPDH as an endogenous control. The kinetics of expression of RORγt showed a constant trend during the first and third week of life, reaching its maximum expression in animals from 2 to 7 days of age, unlike IL-17 whose expression was progressive with age, evidencing a high stimulation and expressio...

El objetivo del estudio fue determinar los niveles relativos de expresión de ARN mensajeros de RORγt e IL-17 en mucosa intestinal de crías de alpacas clínicamente sanas. Se formaron tres grupos etarios conformados por alpacas de 2 a 7 días (grupo 1, n=5), 8 a 20 días (grupo 2, n=6) y 26 a 47 días (grupo 3, n=6), sin distinción de sexo o raza. Se tomaron muestras del segmento medio del yeyuno, se extrajo el ARN total y se empleó la técnica RT-PCR con cebadores. La expresión relativa de ARNm de RORγt e IL-17 fue determinada por el método 2-∆∆Ct usando como calibradores dos crías neonatas y empleándose como control endógeno a GAPDH. La cinética de expresión de RORγt mostró una tendencia constante durante la primera y tercera semana de vida, alcanzando su máxima expresión en los animales de 2 a 7 días de edad, a diferencia de IL-17 cuya expresión fue progresiva co...

The present study determined the presence of retinoic X receptor isoforms (RXR) genes in the genome of adult alpacas and their expression in the jejunal mucosa of alpaca offspring. Blood leukocyte samples from five adult alpacas were used to detect the genes of the RXRbeta and RXRgamma isoforms by PCR using specific primers, and to determine the expression of the RXR gene isoforms, 35 jejunum samples from young alpacas were used (1-50 days old), to which the real-time RT-PCR test was performed. The results indicate that the alpacas have encoded the RXRbeta and RXRgamma isoforms in their genome and that they have different levels of expression in the jejunal mucosa of baby alpacas. High expression of RXRgamma and RXRbeta was detected. These results indicate that the RXRgamma and RXRbeta receptors are being expressed in the mucosa of the jejunum of baby alpacas.