IMPLEMENTATION OF ANALYTICAL METHODOLOGY FOR QUANTIFICATION OF PROTEINS IN Arthrospira platensis MICROALGAE

• Autores: Flores Ramos, Leenin, Ruiz Soto, Anthony
• Formato: artículo
• Fecha de Publicación: 2017
• Institución: Sociedad Química del Perú
• Repositorio: Revista de la Sociedad Química del Perú
• Lenguaje: español
• OAI Identifier: oai:rsqp.revistas.sqperu.org.pe:article/219
• Enlace del recurso: http://revistas.sqperu.org.pe/index.php/revistasqperu/article/view/219
• Nivel de acceso: acceso abierto
• Materia: Spectrophotometry; microalgae; Box Behnken; Youden-Steiner; Espectrofotometría; microalga

The Hartree-Lowry method was developed and implemented for quantification of protein in Arthorspira platensis microalgae by spectrophotometry UV-Vis. In order to achieve this goal, the protein extraction factors were optimized by Box Behnken design of response surface and the following results of the implementation parameters were obtained: the linearity showed a coefficient of determination (R2 ) greater than 0.995, a coefficient of variation less than 2% for repeatability, a percentage recovery within 94% to 101%, the limit of detection and limit of quantification were 2.36 µg/mL and 7.86 µg/mL respectively, and the robustness showed that some parameters such as sample weight and volume of reagent C presented high influence in the evaluated factors.