Los pacientes con leucemia mieloide aguda y traslocación citogenética t(8;21)(q22;q22); RUNX1/RUNX1T1 se les asocia clínicamente con pronóstico favorable. Sin embargo, se ha descrito que esta traslocación está acompañada con otras alteraciones cromosómicas denominadas alteraciones cromosómicas secundarias (ACSs), se cree que estas alteraciones pueden empeorar el pronóstico en los pacientes. En nuestro estudio se eligieron a 50 pacientes con ACSs, mostrando mayor frecuencia la pérdida de los cromosomas sexuales (PCS) y deleción del(9)(q22), y con menor frecuencia encontramos las trisomías de los cromosomas 4;5;7 y 8, y deleciones en los cromosomas 7;9;11;15 y 21. El impacto de la presencia de las ACSs en los pacientes relacionado al pronóstico se observó que los pacientes < 15 años y > 60 años con ACSs el pronóstico se vuelve vulnerable para ellos, mientras que los pacie...

Objective: Patients with non-small cell lung cancer positive for the anaplastic lymphoma kinase (ALK+) gene mutation who also have mutations in the Kirsten rat sarcoma (KRAS) gene, such as KRAS G12C, are showing resistance to both anaplastic lymphoma kinase (ALK) gene and KRAS inhibitors. Therefore, the interaction between ALK inhibitors and KRAS was analyzed to suggest a synergy between them. Materials and methods: The study performed homology modeling of the KRASwt, KRAS G12C and ALKwt structures. Subsequently, molecular dockings were carried out to determine the binding energy of ALK and KRAS inhibitors and to evaluate the possible interaction of ALK inhibitors with KRAS and the KRAS G12C structure. Finally, the expression in the RAS/MEK pathway was analyzed using the Western Blot technique. Results: The binding energy values show the potential interaction of ALKwt inhibitors, such as...

Objective: PI3K is one of the most frequently mutated proteins in cancer, resulting in changes to its functions in regulating metabolism, immunity, among others. Despite the identification of specific drugs targeting PI3K, significant resistance tothese therapies has been observed. Therefore, the search for new inhibitors is crucial. This project proposes a strategy based on in silico computational tools for screening Food and Drug Administration (FDA)-approved drugs, aiming to evaluate their potential for drug repositioning. Materials and methods: This study obtained the sequence of PI3Kα from UniProt Knowledgebase and its three-dimensional structure from AlphaFold Protein Structure Database, which were then coupled with adenosine triphosphate (ATP) and its selective inhibitors: inavolisib, taselisib, CH5132799, alpelisib and ZSTK474. Drug-protein interaction analysiswas performed usin...