El presente trabajo de investigación se desarrolló con el objetivo de aislar y determinar el potencial como promotoras del crecimiento de plantas de actinomicetos aislados de la rizósfera de malezas asociadas a Zea mays L. "maíz" en Lambayeque, como una alternativa a los fertilizantes químicos que causan contaminación ambiental. Las bacterias se aislaron de la rizósfera de malezas procedentes de campos agrícolas de los distritos de Monsefú (6°52'30" latitud sur y 79°52'09" longitud oeste) y Reque (6°52'00" latitud sur y 79°49'27" longitud oeste), Region Lambayeque. Se realizó una dilución en solución salina esterilizada: 0,85% NaCI, p/v y se sembró en agar avena, incubando a 30 °C durante 5 días. Los actinomicetos se reconocieron por las características del micelio aéreo y de sustrato, la pigmentación y la morfología de sus esporóforos, obteniéndose 200 cultivos ...

Introduction. Dendritic cells (DCs) play a key role in antigen presentation and T cell activation, but their function can be modulated by the tumor microenvironment, affecting the antitumor immune response. This study focuses on the interaction between DCs and hepatocellular carcinoma (HCC), exploring how the tumor environment influences DC activity. Objective. To evaluate the variation in DC activity in response to the expression of proinflammatory cytokines, IL-10 and CXCR4 and CCR7 receptors   in a murine model of hepatocellular carcinoma (PM299L). Methods. In vitro assays were performed co-culturing murine DCs and HCC tumor line. The expression of proinflammatory cytokines (IL-12, IL- 6, IL-1β, TNF-α), immunosuppressive (IL-10) and receptors associated with migration and maturation (CXCR4 and CCR7) was evaluated by Qpcr at 24, 48 and 72 hours. The tests were repeated three times....

Objective: Patients with non-small cell lung cancer positive for the anaplastic lymphoma kinase (ALK+) gene mutation who also have mutations in the Kirsten rat sarcoma (KRAS) gene, such as KRAS G12C, are showing resistance to both anaplastic lymphoma kinase (ALK) gene and KRAS inhibitors. Therefore, the interaction between ALK inhibitors and KRAS was analyzed to suggest a synergy between them. Materials and methods: The study performed homology modeling of the KRASwt, KRAS G12C and ALKwt structures. Subsequently, molecular dockings were carried out to determine the binding energy of ALK and KRAS inhibitors and to evaluate the possible interaction of ALK inhibitors with KRAS and the KRAS G12C structure. Finally, the expression in the RAS/MEK pathway was analyzed using the Western Blot technique. Results: The binding energy values show the potential interaction of ALKwt inhibitors, such as...

Objective: PI3K is one of the most frequently mutated proteins in cancer, resulting in changes to its functions in regulating metabolism, immunity, among others. Despite the identification of specific drugs targeting PI3K, significant resistance tothese therapies has been observed. Therefore, the search for new inhibitors is crucial. This project proposes a strategy based on in silico computational tools for screening Food and Drug Administration (FDA)-approved drugs, aiming to evaluate their potential for drug repositioning. Materials and methods: This study obtained the sequence of PI3Kα from UniProt Knowledgebase and its three-dimensional structure from AlphaFold Protein Structure Database, which were then coupled with adenosine triphosphate (ATP) and its selective inhibitors: inavolisib, taselisib, CH5132799, alpelisib and ZSTK474. Drug-protein interaction analysiswas performed usin...

Introduction. Metabolic activity determines the phenotype of macrophages, which can be oriented towards activation of defense mechanisms or tissue repair (phenotypic plasticity). Objectives. To evaluate the behavior of the main enzymes involved in the metabolic pathways of energy production in macrophages exposed to two tumor environments. Methods. In vitro experimental study that consisted of measuring the enzymatic activity of hexokinase (HQN), lactate dehydrogenase (LDH), isocitrate dehydrogenase (IDH) and glucose-6-phosphate dehydrogenase (G6P) by spectrophotometric absorbance in macrophages exposed to lung cancer and hepatocarcinoma cells. We compared the medians of enzyme activity between the isolated cell lines and their co-cultures (Kruskal-Wallis (H) test) and the medians of enzyme activity between each co-culture (Mann-Whitney U test). Results. The enzymatic activity of LDH was...

Objective: To describe the behavior of the BCL-2 protein in non-small-cell lung cancer cell models in response to treatment with ALK (anaplastic lymphoma kinase) protein tyrosine kinase inhibitors (crizotinib and alectinib) and their potential dual BCL-2-ALK inhibition. Materials and methods: Three non-small-cell-lung cancer cell models were used: Ba/f3 EML4-ALKWT, Ba/f3 EML4-ALKL1196M and Ba/f3 EML4-ALKG1202R, generated by site-directed mutagenesis. These were treated with crizotinib and alectinib in a dose-responsive manner, and an apoptosis assay was also conducted to confirm pharmacological susceptibility. Subsequently, BCL-2 protein expression was measured under three treatment conditions (no treatment, 100 nM crizotinib and 50 nM alectinib). Finally, a search for BCL-2 and ALK ligands was performed for molecular docking simulation and interaction energy calculation, measured in kca...