El ecosistema del bosque del manglar de Puerto Pizarro (Tumbes, Perú) con sus biotipos más abundantes Rhizophora mangle, Avicennia germinans y Laguncularia racemosa, concibe diversos bienes y servicios al ambiente, entre estos, la captura de carbono, se requiere de información básica para implementar medidas en su utilización, con estrategias de mitigación y valoración por el importante servicio ambiental que ofrecen. El objetivo fue cuantificar y valorar económicamente la reserva de carbono por biotipo durante las estaciones climatológicas. El área de estudio fue de 830,14 ha, la muestra de campo estuvo constituida por 41 parcelas de 1 ha. La metodología de la investigación comprendió los protocolos utilizados por Kauffman et al. (2013) para carbono de manglar, y las tablas de volúmenes para tres especies de mangle utilizadas por Prestegui (2014). Utilizando ecuaciones alo...

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...