1
tesis de maestría
Publicado 2020
Enlace
Enlace
El rendimiento académico universitario a nivel latinoamericano es muy bajo para esta era de globalización donde se pretende que los grados académicos de un país puedan valer en otro. Cualquier profesor universitario puede notar fácilmente que los estudiantes abusan de los aparatos electrónicos hasta para los cálculos más elementales, esto se traduce en baja producción científica y falta de decisiones rápidas cuando más se requieren. Las últimas pruebas Pisa han desnudado la realidad que ya se conocía y muchas de las carencias tienen orígenes muy remotos en el hogar. Muchos autores han buscado tratar el tema del rendimiento académico desde muchos puntos de vista psicológico, afectivo, social y cognitivo; incluso se ha estudiado las diferencias de género, y por ello existen varias definiciones del rendimiento académico. En el presente trabajo trataremos el aspecto cognit...
2
artículo
Publicado 2018
Enlace
Enlace
The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...
3
artículo
Publicado 2018
Enlace
Enlace
The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...