The aim of this study was to evaluate the repellent efficacy for mosquitoes (Anophelex spp) of three doses of neem oil (Azadirachta indica A. Juss) in pigs under field conditions. The research was carried out at a time of year of abundance of mosquitoes in Tumbes, Peru. Forty recently weaned piglets were used, 10 per treatment, kept in indoor cages protected against mosquitoes. For the field test, four piglets per day were exposed randomly in external cages without protection to the mosquito (a different animal for each treatment per day) for 14 days over a period of two months. Neem oil was extracted with the soxhlet apparatus and the formula included 30% glycerin, 1, 2 or 3% neem oil (T1, T2, T3, respectively), and taken to 100% with pharmaceutical alcohol. A dose of 50 ml/piglet distributed throughout the body was applied. The bites and the number of mosquitoes were evaluated on four ...

The aim of this study was to evaluate the repellent efficacy for mosquitoes (Anophelex spp) of three doses of neem oil (Azadirachta indica A. Juss) in pigs under field conditions. The research was carried out at a time of year of abundance of mosquitoes in Tumbes, Peru. Forty recently weaned piglets were used, 10 per treatment, kept in indoor cages protected against mosquitoes. For the field test, four piglets per day were exposed randomly in external cages without protection to the mosquito (a different animal for each treatment per day) for 14 days over a period of two months. Neem oil was extracted with the soxhlet apparatus and the formula included 30% glycerin, 1, 2 or 3% neem oil (T1, T2, T3, respectively), and taken to 100% with pharmaceutical alcohol. A dose of 50 ml/piglet distributed throughout the body was applied. The bites and the number of mosquitoes were evaluated on four ...

This article contains data on the bacterial communities of lagoon sediments with fish potential in the Central Andes of Peru. The surface sediment samples were collected from four lagoons destined for continental water fish farming. DNA extraction was performed from 0.5 g of sample through the Presto(TM) Soil DNA Extraction Kit. Bacterial sequencing of the 16S rRNA amplicon was performed on the DNA extracted from the sediment. At least 36 Phyla bacteria were detected, the bacterial communities being dominated by Proteobacteria, Cyanobacteria, Actinobacteria, Firmicutes, Chloroflexi. These data can be used for predictive analysis to gain a better understanding of the dynamics of bacterial communities in environments under pressure from fish farming. (C) 2020 The Author(s). Published by Elsevier Inc.

The cumulative effects of anthropogenic stress on freshwater ecosystems are becoming increasingly evident and worrisome. In lake sediments contaminated by heavy metals, the composition and structure of microbial communities can change and affect nutrient transformation and biogeochemical cycling of sediments. In this study, bacterial and archaeal communities of lake sediments under fish pressure contaminated with heavy metals were investigated by the Illumina MiSeq platform. Despite the similar content of most of the heavy metals in the lagoon sediments, we found that their microbial communities were different in diversity and composition. This difference would be determined by the resilience or tolerance of the microbial communities to the heavy metal enrichment gradient.

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...

El objetivo de la investigación fue aislar y caracterizar cepas de Vibrio obtenidas de agua marina en la línea de playa y la zona adyacente a la plataforma de extracción petrolera CX-11 del Lote Z1 ubicado a 17 km de la ciudad de La Cruz (Tumbes, Perú). Las cepas fueron aisladas en caldo mineral Bushnell Haas suplementado con petróleo crudo, luego fueron subcultivadas en caldo de tripticasa soya (TSB) hasta obtener colonias puras, las cuales fueron caracterizadas mediante tinción de Gram y las pruebas de oxidasa y catalasa; además fueron identificadas mediante secuenciamiento de un fragmento de sus genes 16S ARNr. Se verificó que las cepas portaran genes de benceno di oxigenasas. Se pudo aislar seis cepas correspondientes a Vibrio fluvialis y Vibrio spp., que portaban los genes indicados. La investigación demostró que en aguas de la línea de playa y zonas adyacentes a la plata...

El objetivo de la investigación fue aislar y caracterizar cepas de Vibrio obtenidas de agua marina en la línea de playa y la zona adyacente a la plataforma de extracción petrolera CX-11 del Lote Z1 ubicado a 17 km de la ciudad de La Cruz (Tumbes, Perú). Las cepas fueron aisladas en caldo mineral Bushnell Haas suplementado con petróleo crudo, luego fueron subcultivadas en caldo de tripticasa soya (TSB) hasta obtener colonias puras, las cuales fueron caracterizadas mediante tinción de Gram y las pruebas de oxidasa y catalasa; además fueron identificadas mediante secuenciamiento de un fragmento de sus genes 16S ARNr. Se verificó que las cepas portaran genes de benceno di oxigenasas. Se pudo aislar seis cepas correspondientes a Vibrio fluvialis y Vibrio spp., que portaban los genes indicados. La investigación demostró que en aguas de la línea de playa y zonas adyacentes a la plata...