The aim of the study was to isolate and identify native lactobacillus microorganisms from the digestive tract of piglets with the possibility of using them for the improvement of the productive performance of piglets. Samples were cultured and purified on Man, Rogosa and Sharpe (MRS) agar. Molecular identification of the bacteria was done by the sequencing the 16S rRNA gene. Four species were identified: Lactobacillus johnsonii, Lactobacillus brevis, Enterococcus hirae and Pediococcus pentosaceus.

The composition and abundance of the bacterial microbiota in three portions of the gastrointestinal tract of a piglet, in the balanced feed and in biological ensilages prepared with residues of aquaculture origin were evaluated by metagenomic analysis. The DNA of the samples was extracted, and sequenced. The greatest bacterial diversity was in the balanced feed (44.7%), with the predominance of Candidatus phytoplasma surgarcane. In the biological silage predominated Lactobacillus casei and Lactococcus lactis. The existing diversity was similar between silage and the concentrate feed. Similarities were found between the bacteria of the three gastrointestinal regions (L. ultunensis, Selenomonas bovis, Prevotella copri, Enterococcus lactis y Campylobacter sp). In the stomach predominated Helicobacter rappini and Campylobacter coli, in the small intestine Selenomonas bovis and Lactobacillus ...

The aim of the study was to isolate and identify native lactobacillus microorganisms from the digestive tract of piglets with the possibility of using them for the improvement of the productive performance of piglets. Samples were cultured and purified on Man, Rogosa and Sharpe (MRS) agar. Molecular identification of the bacteria was done by the sequencing the 16S rRNA gene. Four species were identified: Lactobacillus johnsonii, Lactobacillus brevis, Enterococcus hirae and Pediococcus pentosaceus.

The composition and abundance of the bacterial microbiota in three portions of the gastrointestinal tract of a piglet, in the balanced feed and in biological ensilages prepared with residues of aquaculture origin were evaluated by metagenomic analysis. The DNA of the samples was extracted, and sequenced. The greatest bacterial diversity was in the balanced feed (44.7%), with the predominance of Candidatus phytoplasma surgarcane. In the biological silage predominated Lactobacillus casei and Lactococcus lactis. The existing diversity was similar between silage and the concentrate feed. Similarities were found between the bacteria of the three gastrointestinal regions (L. ultunensis, Selenomonas bovis, Prevotella copri, Enterococcus lactis y Campylobacter sp). In the stomach predominated Helicobacter rappini and Campylobacter coli, in the small intestine Selenomonas bovis and Lactobacillus ...

Se aislaron microrganismos benéficos del tracto digestivo del lechón, utilizando medio lacto agar, caracterizando su genoma mediante su ADN con amplificación PCR encontrándose e identificándose, Enterococcus hirae ATCC 9790, Lactobacillus brevis ATCC 367, Lactobacillus johnsonii NCC 533, Pediococcus pentosaceus ATCC 25745), la mezcla de estos lactobacilus fueron utilizados como inoculo para la preparación del ensilado biológico de residuos de langostino EB, la dosis del inoculo (mezcla de los microrganismos en igual concentración), se evaluó mediante, dosificándolo a lechones pre destetados vía oral, dosis determinada por espectrofotometría, densidad óptica (DO) la mejor concentraciones correspondiente a 1.5 DO equivalente a 3x108 UFC/gr. Obteniendo el mejor ICA equivalente a 0,96. El ensilado biológico de residuos orgánicos de Litopenaeus vannamei (EB) preparado, se evalu...

Una de las actividades más comunes en la alimentación porcina es el uso de antibióticos como aditivo, dañinos para la salud animal y salud pública. Considerando que los animales van adquiriendo microorganismos de su entorno, los cuales colonizan las diferentes regiones del tracto digestivo, logran ser parte de la flora natural y algunas tienen actividad antibiótica. Con la finalidad de identificar y estudiar la actividad antibacteriana de bacterias ácido lácticas (BAL) del tracto digestivo del Sus scrofa domesticus (lechón), se extrajeron muestras mediante laparoscopía, por hisopado del estómago, duodeno, yeyuno, íleon, ciego y colon, a las cuales se les realizó la caracterización morfológica y bioquímica según el método agar caldo Man, Rogosa y Sharpe (MRS) con azul de anilina (Yimin et al. 2005). Se evaluó el efecto bactericida y antibiótico de las bacteriocinas en ...

The study aimed to characterize the antibiotic resistance in Vibrio spp isolated from Litopenaeus vannamei and to test oregano (Origanum vulgare) and neem (Azadirachta indica) extracts for their inhibition. Farmed shrimp were collected in Santa Rosa (Ecuador) and samples of hepatopancreas, hemolymph and lesions were taken. Fourteen strains of Vibrio spp were isolated. Another 14 strains of Vibrio spp isolated from farmed shrimp from Tumbes (Peru) were obtained through a donation. The antibiotic resistance of the strains against 10 antibiotics and the sensitivity of four of them (two resistant and two multi-resistant to antibiotics) were evaluated using neem and oregano aqueous extracts (EA), ethanolic (EE), as well as purified with soxhlet and rota evaporator (EPSR). The 28 strains studied were resistant to at least one of the antibiotics, 64.3% were multi-resistant and 50% were resistan...

The aim of this study was to determine the viability and nutritional characteristics of shrimp head silage and fish waste destined for pig feeding. In the conservation process, molasses and rice powder were used as a substrate and Lactobacullus fermentus, obtained from the digestive tract of the piglet, as a fermenter. Temperature, pH and percentage of acidity data were recorded, as well as organoleptic and nutritional characteristics in the first 30 days of conservation. The treatments were T1 = Shrimp head with molasses and yogurt; T2 = Shrimp head with rice powder and yogurt; T3 = Fish residue with molasses and yogurt; and T4 = Fish residue with rice powder and yogurt. T1 and T3 presented acidity and low pH, as well as low temperature during the first week, stabilizing the process on day 15. The fermentation of T2 and T4 was inadequate (not feasible). Aerobic mesophilic microorganisms...

The aim of this study was to determine the viability and nutritional characteristics of shrimp head silage and fish waste destined for pig feeding. In the conservation process, molasses and rice powder were used as a substrate and Lactobacullus fermentus, obtained from the digestive tract of the piglet, as a fermenter. Temperature, pH and percentage of acidity data were recorded, as well as organoleptic and nutritional characteristics in the first 30 days of conservation. The treatments were T1 = Shrimp head with molasses and yogurt; T2 = Shrimp head with rice powder and yogurt; T3 = Fish residue with molasses and yogurt; and T4 = Fish residue with rice powder and yogurt. T1 and T3 presented acidity and low pH, as well as low temperature during the first week, stabilizing the process on day 15. The fermentation of T2 and T4 was inadequate (not feasible). Aerobic mesophilic microorganisms...

The aim of this study was to determine the viability and use of the biological silage (BE) of shrimp head, molasses and milk fermented with lactic acid bacteria (75-25-5, respectively), providing it as feed for backyard pigs and its leachate as organic fertilizer in the cultivation of maralfalfa grass. It was tested by adding to a base diet for pigs, daily at levels of 0%, 5%, 10 and 15% of the BE and as fertilizer in doses of 300, 500, 700 L/ha (leached) and control (100 nitrogen units) in three applications at 0, 30 and 60 days of installation. Vegetative seed was used in 16 pools of 22 m2, evaluating the main productive parameters at 30, 60 days and at harvest (90 days), 40 growing animals (20 kg) were evaluated in pigs for 35 days. The EB and its leachate were viable and stabilized after 15 days of preparation (pH less than 4.5), presented adequate values as feed for pigs according t...

The aim of this study was to determine the viability and use of the biological silage (BE) of shrimp head, molasses and milk fermented with lactic acid bacteria (75-25-5, respectively), providing it as feed for backyard pigs and its leachate as organic fertilizer in the cultivation of maralfalfa grass. It was tested by adding to a base diet for pigs, daily at levels of 0%, 5%, 10 and 15% of the BE and as fertilizer in doses of 300, 500, 700 L/ha (leached) and control (100 nitrogen units) in three applications at 0, 30 and 60 days of installation. Vegetative seed was used in 16 pools of 22 m2, evaluating the main productive parameters at 30, 60 days and at harvest (90 days), 40 growing animals (20 kg) were evaluated in pigs for 35 days. The EB and its leachate were viable and stabilized after 15 days of preparation (pH less than 4.5), presented adequate values as feed for pigs according t...

The aim of this study was to evaluate the repellent efficacy for mosquitoes (Anophelex spp) of three doses of neem oil (Azadirachta indica A. Juss) in pigs under field conditions. The research was carried out at a time of year of abundance of mosquitoes in Tumbes, Peru. Forty recently weaned piglets were used, 10 per treatment, kept in indoor cages protected against mosquitoes. For the field test, four piglets per day were exposed randomly in external cages without protection to the mosquito (a different animal for each treatment per day) for 14 days over a period of two months. Neem oil was extracted with the soxhlet apparatus and the formula included 30% glycerin, 1, 2 or 3% neem oil (T1, T2, T3, respectively), and taken to 100% with pharmaceutical alcohol. A dose of 50 ml/piglet distributed throughout the body was applied. The bites and the number of mosquitoes were evaluated on four ...

The aim of this study was to evaluate the repellent efficacy for mosquitoes (Anophelex spp) of three doses of neem oil (Azadirachta indica A. Juss) in pigs under field conditions. The research was carried out at a time of year of abundance of mosquitoes in Tumbes, Peru. Forty recently weaned piglets were used, 10 per treatment, kept in indoor cages protected against mosquitoes. For the field test, four piglets per day were exposed randomly in external cages without protection to the mosquito (a different animal for each treatment per day) for 14 days over a period of two months. Neem oil was extracted with the soxhlet apparatus and the formula included 30% glycerin, 1, 2 or 3% neem oil (T1, T2, T3, respectively), and taken to 100% with pharmaceutical alcohol. A dose of 50 ml/piglet distributed throughout the body was applied. The bites and the number of mosquitoes were evaluated on four ...

This article contains data on the bacterial communities of lagoon sediments with fish potential in the Central Andes of Peru. The surface sediment samples were collected from four lagoons destined for continental water fish farming. DNA extraction was performed from 0.5 g of sample through the Presto(TM) Soil DNA Extraction Kit. Bacterial sequencing of the 16S rRNA amplicon was performed on the DNA extracted from the sediment. At least 36 Phyla bacteria were detected, the bacterial communities being dominated by Proteobacteria, Cyanobacteria, Actinobacteria, Firmicutes, Chloroflexi. These data can be used for predictive analysis to gain a better understanding of the dynamics of bacterial communities in environments under pressure from fish farming. (C) 2020 The Author(s). Published by Elsevier Inc.

The cumulative effects of anthropogenic stress on freshwater ecosystems are becoming increasingly evident and worrisome. In lake sediments contaminated by heavy metals, the composition and structure of microbial communities can change and affect nutrient transformation and biogeochemical cycling of sediments. In this study, bacterial and archaeal communities of lake sediments under fish pressure contaminated with heavy metals were investigated by the Illumina MiSeq platform. Despite the similar content of most of the heavy metals in the lagoon sediments, we found that their microbial communities were different in diversity and composition. This difference would be determined by the resilience or tolerance of the microbial communities to the heavy metal enrichment gradient.

Neoraimondia arequipensis subsp. gigantea is registered in the dry forests of Tumbes, expanding its geographical distribution in northern Peru, based on the phenotypic identification of 70 individuals and genotypic by sequencing the matK gene (Maturase K gene). The habitat of this species is characterized by the presence of stony and rocky soil, located adjacent to areas of ravines and altitudes between 20 to 60 meters above sea level. In addition, we include the record of biological interactions of this cactus with endemic fauna and flora of the department of Tumbes.

Neoraimondia arequipensis subsp. gigantea is registered in the dry forests of Tumbes, expanding its geographical distribution in northern Peru, based on the phenotypic identification of 70 individuals and genotypic by sequencing the matK gene (Maturase K gene). The habitat of this species is characterized by the presence of stony and rocky soil, located adjacent to areas of ravines and altitudes between 20 to 60 meters above sea level. In addition, we include the record of biological interactions of this cactus with endemic fauna and flora of the department of Tumbes.

Cerca de la mitad de las tierras continentales del planeta son consideradas áridas o sufren amenaza de sequía. Dentro de las plantas adaptadas a estos ecosistemas destacan los cactus que cuentan con varios mecanismos de adaptación, en particular relacionados a su asociación con comunidades microbianas benéficas a nivel de su rizósfera. Entre ellos destacan las rizobacterias promotoras del crecimiento vegetal (RPCV) y los hongos micorrízicos arbusculares (HMA) conocidos para contribuir al desarrollo exitoso de la planta en condiciones de aridez. La identificación molecular de bacterias y hongos HMA, aislados de la rizósfera de O. ficus-indica de cinco zonas áridas del departamento de Tumbes, ha sido realizada por secuenciación parcial de rDNA. Plantas de cactus cultivadas in vitro han sido establecidas e inoculadas con las cepas microbianas previamente caracterizadas. Ningún e...

Cerca de la mitad de las tierras continentales del planeta son consideradas áridas o sufren amenaza de sequía. Dentro de las plantas adaptadas a estos ecosistemas destacan los cactus que cuentan con varios mecanismos de adaptación, en particular relacionados a su asociación con comunidades microbianas benéficas a nivel de su rizósfera. Entre ellos destacan las rizobacterias promotoras del crecimiento vegetal (RPCV) y los hongos micorrízicos arbusculares (HMA) conocidos para contribuir al desarrollo exitoso de la planta en condiciones de aridez. La identificación molecular de bacterias y hongos HMA, aislados de la rizósfera de O. ficus-indica de cinco zonas áridas del departamento de Tumbes, ha sido realizada por secuenciación parcial de rDNA. Plantas de cactus cultivadas in vitro han sido establecidas e inoculadas con las cepas microbianas previamente caracterizadas. Ningún e...