The study aimed to characterize the antibiotic resistance in Vibrio spp isolated from Litopenaeus vannamei and to test oregano (Origanum vulgare) and neem (Azadirachta indica) extracts for their inhibition. Farmed shrimp were collected in Santa Rosa (Ecuador) and samples of hepatopancreas, hemolymph and lesions were taken. Fourteen strains of Vibrio spp were isolated. Another 14 strains of Vibrio spp isolated from farmed shrimp from Tumbes (Peru) were obtained through a donation. The antibiotic resistance of the strains against 10 antibiotics and the sensitivity of four of them (two resistant and two multi-resistant to antibiotics) were evaluated using neem and oregano aqueous extracts (EA), ethanolic (EE), as well as purified with soxhlet and rota evaporator (EPSR). The 28 strains studied were resistant to at least one of the antibiotics, 64.3% were multi-resistant and 50% were resistan...