The aim of this study was to compare the capacity of embryonic development of oocytes recovered from slaughterhouse ovaries and recovered in vivo by guided ultrasound (ovum pick-up – OPU) in an in vitro production system. Nine sessions in 59 Holstein cows were done to obtain oocytes through OPU and 408 ovaries were collected from 204 cows in the slaughterhouse. For the in vitro maturation of oocytes, only those of grade 1 and 2 were used, matured and fertilized using MIV, FIV and CIV® medium until day 7. The recovery rate of oocytes, grade 1 and 2 oocytes and the percentage of embryos (blastocysts) produced in vitro on day 7 were evaluated. The recovery rate of oocytes collected from slaughterhouse ovaries was higher as compared with oocytes collected by OPU (10.0 vs. 7.2 oocytes per session/cow, p<0.05). There was a greater number of viable oocytes of grade 1 and 2 when the collec...

El objetivo del presente estudio fue comparar la capacidad de desarrollo embrionario de ovocitos recuperados de ovarios de matadero y recuperados in vivo mediante ultrasonografía guiada (ovum pick-up, OPU) en un sistema de producción in vitro. Se realizaron nueve sesiones de colecta en 59 vacas Holstein para la obtención de ovocitos mediante OPU y se colectaron 408 ovarios de 204 vacas de matadero. Para la maduración in vitro de ovocitos solo se utilizaron aquellos de grado 1 y 2 mediante medios MIV, FIV y CIV® hasta el día 7. Se evaluó la tasa de recuperación de ovocitos, ovocitos grado 1 y 2 y el porcentaje de embriones (blastocitos) producidos in vitro al día 7. La tasa de recuperación de ovocitos fue mayor cuando se colectaron de ovarios de matadero comparado con ovocitos colectados por OPU (10.0 vs. 7.2 ovocitos por sesión/vaca; p<0.05). Hubo una mayor cantidad de ovoc...

Choosing Wisely is an initiative by the American Board of Internal Medicine (ABIM) and ABIM Foundation to deter unnecessary medical treatments and procedures. Faced with the burden of modern technologies and treatments, it is crucial to identify practices lacking value in daily care. The Latin American and Caribbean Society (SLACOM), comprising cancer control experts, deems it vital to tailor this initiative for enhancing cancer care in the region. Through a modified DELPHI methodology involving two rounds of electronic questionnaires and a hybrid meeting to discuss key points of contention, ten essential recommendations were identified and prioritised to avoid harmful oncology procedures in our region. These consensus-based recommendations, contextualised for Latin America, have been compiled and shared to benefit patients. The Scientific Committee, consisting of prominent oncologists a...