Taenia solium cysticercosis is a serious public health problem in developing countries around the world. At the present time, treatment with anthelmintic drugs is possible, but once applied the treatment the monitoring of the animal is limited by the high cost of the radiological techniques. Moreover, the available immunodiagnostic assays are not useful for monitoring because they detect the presence of antibodies against C. cellulosae, which remain in circulation after cysts are dead. The objective of the present study was to demonstrate the usefulness of a monoclonal antibody-based capture ELISA (AgELISA) in the monitoring of the persistence of circulating parasite antigens in animals treated against cysticercosis. For this purpose, serum samples corresponding to the monitoring of pre and post treatment of 6 pigs, naturally infected with cysticercosis and treated with oxfendazol, were ...

The aim of this study was to evaluate the presence of avian influenza (AI) virus in backyard domestic ducks in the provinces of Huaral and Huaura (Lima, Peru). Six-hundred cloacal swab specimens were collected and analyzed by virus isolation in chick embryo eggs free from specific pathogens (SPF). The presence of the AI virus was determined by the hemagglutinating activity of the allantoic fluid and confirmed by a commercial kit using monoclonal antibodies. All samples analyzed were negative to the AI virus. The risk of the disease was evaluated using the Monte Carlo simulation (@risk program) which determined that the AI virus would show a maximum stochastic prevalence of 0.33% with a 95% confidence interval in the event of the disease. It was concluded that the birds included in the present study were not infected with the AI virus.

La cisticercosis por Taenia solium es una enfermedad zoonótica común en países en desarrollo y un grave problema de salud pública a nivel mundial. En la actualidad es posible tratarla mediante el uso de cisticidas, pero una vez aplicado el tratamiento, el monitoreo de la evolución del animal es limitado por el alto costo de las técnicas de radioimagen. Además, las pruebas inmunodiagnósticas disponibles no son útiles en el monitoreo pues detectan la presencia de anticuerpos contra los C. cellulosae, los cuales se mantienen en circulación, aún después de la muerte de los quistes. Por ello, el objetivo del presente estudio fue demostrar la utilidad del ELISA captura de antígeno en el monitoreo de la persistencia de antígeno parasitario circulante en animales tratados contra cisticer-cosis. Para este fin, se tomó un grupo de sueros que corresponden al seguimiento pre y post tr...

El objetivo del estudio fue evaluar la presencia del virus de influenza aviar (IA) en patos domésticos de crianza familiar en las provincias de Huaral y Huaura (Lima, Perú). Se colectaron 600 muestras de hisopado cloacal de patos domésticos de traspatio, que fueron analizadas mediante aislamiento viral en huevos embrionados de pollo libres de patógenos específicos (SPF). La presencia del virus de IA fue determinada por la actividad hemaglutinante del fluido alantoideo y confirmada mediante un kit comercial que utiliza anticuerpos monoclonales. Todas las muestras analizadas fueron negativas al virus de IA. El riego de infección fue evaluado mediante la simulación de Monte Carlo (programa @risk) determinando que el virus de IA mostraría una prevalencia estocástica máxima de 0.33% con un intervalo de confianza de 95% en caso de presentarse la enfermedad. Se concluye que las aves i...

The objective of the present study was to evaluate the age of alpacas as a risk factor to become infected with Sarcocystis sp. Blood samples were collected from 941 alpacas reared at the SAIS Túpac Amaru (Junín, Peru). Samples were stratified for statistical analysis in 5 age groups (<1, 1, 2, 3, ? 4 years). The sera were analyzed by the indirect ELISA test for the detection of Sarcocystis antibodies. The results showed 844 positive samples representing a seroprevalence of 89.7 ± 1.9% (46, 98, 96, 95 and 95% for < 1, 1, 2, 3, and ? 4 years old, respectively). There were statistical differences between age and the presence of Sarcocystis sp. antibodies (p<0.05).

The objective of the present study was to evaluate the age of alpacas as a risk factor to become infected with Sarcocystis sp. Blood samples were collected from 941 alpacas reared at the SAIS Túpac Amaru (Junín, Peru). Samples were stratified for statistical analysis in 5 age groups (<1, 1, 2, 3, ? 4 years). The sera were analyzed by the indirect ELISA test for the detection of Sarcocystis antibodies. The results showed 844 positive samples representing a seroprevalence of 89.7 ± 1.9% (46, 98, 96, 95 and 95% for < 1, 1, 2, 3, and ? 4 years old, respectively). There were statistical differences between age and the presence of Sarcocystis sp. antibodies (p<0.05).