Listeriosis primarily affects pregnant women, newborns, elderly people and patients with low immune response, among others, and is caused by Listeria monocytoges. In this study we characterized molecularly 20 strains of L. monocytogenes isolated from 18 cases of perinatal listeriosis in Hospital San Bartolomé of Lima-Perú, during 2001 to 2005 years. The bacteria were characterized initially by conventional bacteriological testing, such as β-hemolysis incomplete, CAMP reaction positive with Staphylococcus aureus and negative with Rhodococcus equi and the differential utilization of carbohydrates as D-glucose and L-rhamnose, but not D-xylose and D-mannitol. Then, strains were identified by polymerase chain reaction (PCR) and typified by the technique of Random Amplified Polymorphic DNA (RAPD) primers using OMP-01, M13, M132, PJ108 and PJ108. With primers OMP-01 and PJ108 were obtained s...

Listeriosis primarily affects pregnant women, newborns, elderly people and patients with low immune response, among others, and is caused by Listeria monocytoges. In this study we characterized molecularly 20 strains of L. monocytogenes isolated from 18 cases of perinatal listeriosis in Hospital San Bartolomé of Lima-Perú, during 2001 to 2005 years. The bacteria were characterized initially by conventional bacteriological testing, such as β-hemolysis incomplete, CAMP reaction positive with Staphylococcus aureus and negative with Rhodococcus equi and the differential utilization of carbohydrates as D-glucose and L-rhamnose, but not D-xylose and D-mannitol. Then, strains were identified by polymerase chain reaction (PCR) and typified by the technique of Random Amplified Polymorphic DNA (RAPD) primers using OMP-01, M13, M132, PJ108 and PJ108. With primers OMP-01 and PJ108 were obtained s...

OBJETIVO: Determinar la resistencia de los patógenos respiratorios a diferentes antimicrobianos. MATERIAL Y MÉTODOS: Entre abril y noviembre de 2002 se estudió 177 pacientes que asistieron al consultorio externo de otorrinolaringología del Hospital Nacional Docente Madre-Niño San Bartolomé. RESULTADOS: Streptococcus pneumoniae fue la bacteria patógena más aislada (57,2%), luego Moraxella catarrhalis (42,7%), Staphylococcus aureus (18,6%) y en pequeña cantidad Haemophilus influenzae (3,4%) y Streptococcus pyogenes (0,7%). Streptococcus pneumoniae presentó 31,3% de resistencia a la penicilina. El 96,7% de Moraxella catarrhalis fueron productoras de betalactamasa y 7,4% de los Staphylococcus aureus fueron resistentes a la oxacilina. CONCLUSIÓN: Streptococcus pneumoniae es el principal agente causal de los procesos infecciosos altos en niños y su resistencia a la penicilina aumen...

OBJECTIVE: To determine the presence of congenital rubella syndrome (CRS) in three Peruvian cities. DESIGN: Transversal, descriptive study. MATERIAL AND METHODS: Children under twelve years old with major definition criteriae of congenital rubella syndrome receiving special education in Lima (three), Cusco (two) and Arequipa (one) schools, from 1998 to 2000, were included. RESULTS: From 364 children 105 were included, 61% of cases suspicious, 21% probable and 1,9% confirmed; 64,7% had central nervous system manifestations, 31% ocular manifestations, 18% at least two clinical manifestations. CONCLUSIONS: Probable and confirmed cases of CRS represented 6,6% of all children receiving special education, evidencing that CRS represents a health problem in our country and the need to initiate anti-rubella vaccination in Peru.

Background: An ESBL Salmonella typhimurium outbreak occurred at San Bartolome hospital from February 17 through March 16, 2001. Objectives: To identify the mechanism involved in Salmonella typhimurium spread and genetic characterization of beta-lactamase resistance associated genes. Design: Clinical-bacteriologic retrospective study. Setting: San Bartolomé Mother Child Teaching National Hospital. Biologic materials: Bacterial isolations from lactating patients. Interventions: The genetic diversity was characterized from five bacterial isolates from infants admitted to the pediatric units, using REP-PCR and plasmid fingerprinting. We previously characterized the antimicrobial resistance, determining the presence of ESBL by the double disc diffusion method and the variant was identified by sequencing the gen blashv . Main outcome measures: Salmonella typhimurium genotypes, plasmids and be...

Objective: To determine congenital rubella syndrome (CRS) presence in Peru. Design: Descriptive, cross sectional study. Material and Methods: Review of clinical records of children hospitalized between January 1998 and June 2000 at 17 hospital pediatric services in seven districts of Peru, children who presented al least one of the following diagnosis: congenital cataracts or glaucoma, retinitis pigmentosa, congenital cardiac malformation or neurosensorial deafness. The cases were classified as suspected, probable and confirmed. Results: From 91102 hospital discharge diagnosis of children less than 12 year-old, 361 fulfilled inclusion criteria; 51,8% were suspicious, 9,7% probable, and 1,4% confirmed; 93,6% had congenital heart symptomatology, 29,6% central nervous alterations, and 18,6% ocular manifestations;11% of the children had at least two clinical manifestations. Conclusion: There...