Pyrazinamide (PZA) is an antibiotic used in first- and second-line tuberculosis treatment regimens. Approximately 50% of multidrug-resistant tuberculosis and over 90% of extensively drug-resistant tuberculosis strains are also PZA resistant. Despite the key role played by PZA, its mechanisms of action are not yet fully understood. It has been postulated that pyrazinoic acid (POA), the hydrolyzed product of PZA, could inhibit trans-translation by binding to Ribosomal protein S1 (RpsA) and competing with tmRNA, the natural cofactor of RpsA. Subsequent data, however, indicate that these early findings resulted from experimental artifact. Hence, in this study we assess the capacity of POA to compete with tmRNA for RpsA. We evaluated RpsA wild type (WT), RpsA A438, and RpsA A438 variants with truncations towards the carboxy terminal end. Interactions were measured using Nuclear Magnetic Reson...

Background: The Amazonian forests are increasingly threatened due to continuous changes in land use, particularly deforestation. This study aimed to quantify and analyze the vertical distribution of soil glomalin and its relationship with carbon, climate, and soil properties across three forest types of the Peruvian Amazon. A total of 18 plots were selected and sampled in forests with different vegetation cover types: deforested, disturbed, and primary forest. The vertical variation of total glomalin (TG), easily extractable glomalin (EEG), and the number of arbuscular mycorrhizal fungal (AMF) spores was estimated, as it was the relationships of these variables with soil depth, physical-chemical properties, and climate conditions. Results: The mean values for TG, EEG, and AMF showed vertical variations in the three forest cover types, with high values in disturbed forests and degraded so...