This paper reports on biochemical characteristics, hemolytic activity, cytolytic and citotóxic of three fractions venom from the sea anemone Anthothoe chilensis. The arms of 78 individuals of A. chilensis, from the Isle Cabinza-San Lorenzo, Lima, were treated to obtain a filtrate (Ft), which is divided by precipitation with three points of saturation with cold acetone: I (20%), II (50%), III (80%). The Ft showed 1,8 mg/mL of protein concentration. Test of total carbohydrate detection showed the presence of 1,401 mg of glucose/ml of solution in Ft. SDS-PAGE showed proteins between 14 to 94 kDa, almost were glycoproteins. Hemolytic activity was assayed on human erythrocytes showing positive result the fractions II and I. The fraction III was highest phospholipasic activity. Evaluating the proteolytic activity on casein was observed that the fraction I has the highest activity. Cytotoxic a...

El presente trabajo informa sobre características bioquímicas, actividad hemolítica, citotóxica y citolítica de tres fracciones del veneno de la anémona de mar Anthothoe chilensis. Los tentáculos de 78 ejemplares de A. chilensis, provenientes de la Isla Cabinza-San Lorenzo, Lima, fueron procesados obteniendo un filtrado, el cual se fraccionó por precipitación con tres puntos de saturación con acetona fría: I (20%), II (50%), III (80%). El filtrado mostró una concentración proteica de 1,8 mg/mL. Las pruebas de detección de carbohidratos totales demostraron la presencia de 1,401 mg de glucosa/ml de solución en el filtrado; mientras que la electroforesis en gel de poliacrilamida en presencia de dodecil sulfato de sodio (PAGE-SDS) evidenció proteínas de 14 a 94 kDa, de las cuales la mayor parte fueron glicoproteínas. Se encontró actividad hemolítica sobre eritrocitos huma...

Se describen los cambios tempranos en huevos de Thais chocolata, durante su desarrollo y hasta el momento que las larvas eclosionan, a una temperatura de 22,5°C así como las oviposturas, bajo condiciones de laboratorio. Se presentan huevos esféricos, con diámetro promedio de 142,59u. Se observá un gran lóbulo polar en asociación con la primera segmentación. A la semana se encuentran larvas en trocófera y a los 13 días larvas prevelíger que presentan esbozo de conchilla. La etapa intracapsular culmina en 21 días, cuando las cápsulas eclosionan liberando al mar larvas velíger, con estómago larval velum desarrollado y bilobulado.

Se describen los cambios tempranos en huevos de Thais chocolata, durante su desarrollo y hasta el momento que las larvas eclosionan, a una temperatura de 22,5°C así como las oviposturas, bajo condiciones de laboratorio. Se presentan huevos esféricos, con diámetro promedio de 142,59u. Se observá un gran lóbulo polar en asociación con la primera segmentación. A la semana se encuentran larvas en trocófera y a los 13 días larvas prevelíger que presentan esbozo de conchilla. La etapa intracapsular culmina en 21 días, cuando las cápsulas eclosionan liberando al mar larvas velíger, con estómago larval velum desarrollado y bilobulado.

Presenta los resultados obtenidos en el estudio del desarrollo embrionario del gastrópodo: Thais chocolata, conocido como caracol común, entre julio y noviembre de 1994, cuyo objetivo principal es investigar los aspectos intracapsulares, biología postlarval y usar algunos elementos del cultivo de moluscos bivalvos para examinar la factibilidad de su cultivo en masa.

The DNA replication is disrupted by Busulfan which is a bifunctional alquilant as an ester agent. High dose of this compound are applied before transplants of bone marrow and germ cells. However quantification of the damage to the reproductive system and its recovery have not been established. The aim of this study was to quantify both spermatogenesis and spermiogenesis recovery in mice from damage caused by an injection of Busulfan. We used Balb C mice which were evaluated 30, 54 and 72 days after the intraperitoneal injection of 40 mg/kg body weight. We took the following variables: testicular weight, spermatic concentration in epididimus line and histological cuts to measure the diameter of the seminiferous tubules. Results were analyzed with the test U Mann-Whitney p>0.05. By day 30, testicular weight, spermatic concentration and diameter of the seminiferous tubules were reduced t...

The DNA replication is disrupted by Busulfan which is a bifunctional alquilant as an ester agent. High dose of this compound are applied before transplants of bone marrow and germ cells. However quantification of the damage to the reproductive system and its recovery have not been established. The aim of this study was to quantify both spermatogenesis and spermiogenesis recovery in mice from damage caused by an injection of Busulfan. We used Balb C mice which were evaluated 30, 54 and 72 days after the intraperitoneal injection of 40 mg/kg body weight. We took the following variables: testicular weight, spermatic concentration in epididimus line and histological cuts to measure the diameter of the seminiferous tubules. Results were analyzed with the test U Mann-Whitney p>0.05. By day 30, testicular weight, spermatic concentration and diameter of the seminiferous tubules were reduced t...

Aim: The antimicrobial activity of Passiflora mollissima is largely due to its aliphatic and aromatic hydrocarbons, terpenes, alkaloids, ketones, esters, alcohols, and lactones. The objective of this research was to evaluate the antimicrobial activity of the compounds in the Passiflora mollissima (Tumbo) fruit and leaves included also on cultured strains of the microorganisms Streptococcus mutans, Streptococcus oralis, Streptococcus sanguinis and Candida albicans. Materials and methods: Antimicrobial activity was analyzed by the disk diffusion method and evaluated in terms of their zones of inhibition. The cytotoxic activity of these compounds was determined in a bioassay involving sea urchin eggs (Tetrapygusniger) in which the inhibition percentage of artificially fertilized ovules was equivalent to their cytotoxic activity after 26 hours. Results: This study demonstrated the antimicrob...