El presente trabajo de investigación, tiene como objetivo encontrar una alternativa de biocontrol contra el nemátodo del nudo (Meloidogyne incognita), mediante la utilización de 7 géneros de hongos endófitos asociados al cultivo de sacha inchi (Plukenetia spp); para lo cual se evaluó el porcentaje de parasitismo hacia huevos y estadios juveniles (J2) y el porcentaje de eclosión de huevos de M. incognita sometidos a extractos fúngicos de micoendofíticos de sacha inchi. Se trabajaron con 20 tratamientos (4 cepas de Clonostachys sp; 4 cepas de Trichoderma sp; 4 cepas de Pestalotiopsis sp; 3 cepas de Xylaria sp; 2 cepas de Penicillium sp; 2 cepas de Phyalophora sp y 1 cepa de Acremonium sp) y un control. De los cuales para la prueba de parasitismo se trabajó con 21 tratamientos y 3 repeticiones, inoculando 500 huevos de M. incognita/50 g de suelo estéril y conidias de hongos endó...

Coffee leaf rust (Hemileia vastatrix) is the most important disease in coffee crops around the world. Currently, there is limited knowledge about mass production methodologies and ex-situ infection of H. vastatrix that lead to the development of different health characterization studies of Coffea or disease phenotyping. In this context, the aim of this research was to develop a simple protocol for the mass production of coffee rust uredospores under controlled conditions and to determine their infection in coffee seedlings. Uredospores of H. vastatrix were collected from infected plants in the “Chontal” coffee-growing area in San Martín, Peru. The viability of uredospore germination was evaluated, and a uredospores suspension (2 x 105 uredospores/mL) was prepared to inoculate coffee seedlings. Incidence and sporulation rates were evaluated after 43 days of inoculation. During the mu...

Coffee leaf rust (Hemileia vastatrix) is the most important disease in coffee crops around the world. Currently, there is limited knowledge about mass production methodologies and ex-situ infection of H. vastatrix that lead to the development of different health characterization studies of Coffea or disease phenotyping. In this context, the aim of this research was to develop a simple protocol for the mass production of coffee rust uredospores under controlled conditions and to determine their infection in coffee seedlings. Uredospores of H. vastatrix were collected from infected plants in the “Chontal” coffee-growing area in San Martín, Peru. The viability of uredospore germination was evaluated, and a uredospores suspension (2 x 105 uredospores/mL) was prepared to inoculate coffee seedlings. Incidence and sporulation rates were evaluated after 43 days of inoculation. During the mu...