El presente trabajo se realizó en el departamento de San Martin, distrito y provincia de Lamas. El objetivo fue evaluar el efecto de la aplicación de materia orgánica y sistemas de cultivos en el mejoramiento de las características físicas, químicas y biológicas del suelo. Metodología, el experimento se instaló en el fundo “El Pacifico”, terreno con antecedente de 30 años con cultivos de hortalizas. Se utilizó biomasa de repollo morado con dosis de 40 t.ha-1 y 80 t.ha-1 en 5 sistemas de cultivos hortícolas acondicionados mediante siembra directa, utilizándose 6 hortalizas combinadas (culantro, cebolla china, lechuga, frijol y rabanito). El diseño empleado fue DBCA con 10 tratamientos A1B1 (40 t.ha-1 x Cebolla, Lechuga y Rabanito), A1B2 (40 t.ha-1 x Asociación Cebolla china + frejol, asociación lechuga + frejol y asociación rabanito + frejol), A1B3 (40 t.ha-1 x A. ceb...

Coffee leaf rust (Hemileia vastatrix) is the most important disease in coffee crops around the world. Currently, there is limited knowledge about mass production methodologies and ex-situ infection of H. vastatrix that lead to the development of different health characterization studies of Coffea or disease phenotyping. In this context, the aim of this research was to develop a simple protocol for the mass production of coffee rust uredospores under controlled conditions and to determine their infection in coffee seedlings. Uredospores of H. vastatrix were collected from infected plants in the “Chontal” coffee-growing area in San Martín, Peru. The viability of uredospore germination was evaluated, and a uredospores suspension (2 x 105 uredospores/mL) was prepared to inoculate coffee seedlings. Incidence and sporulation rates were evaluated after 43 days of inoculation. During the mu...

Coffee leaf rust (Hemileia vastatrix) is the most important disease in coffee crops around the world. Currently, there is limited knowledge about mass production methodologies and ex-situ infection of H. vastatrix that lead to the development of different health characterization studies of Coffea or disease phenotyping. In this context, the aim of this research was to develop a simple protocol for the mass production of coffee rust uredospores under controlled conditions and to determine their infection in coffee seedlings. Uredospores of H. vastatrix were collected from infected plants in the “Chontal” coffee-growing area in San Martín, Peru. The viability of uredospore germination was evaluated, and a uredospores suspension (2 x 105 uredospores/mL) was prepared to inoculate coffee seedlings. Incidence and sporulation rates were evaluated after 43 days of inoculation. During the mu...