Objetivo: Determinar el efecto que ejerce la administración de hierro hemo y la de sulfato ferroso con vitamina C en hígado y cerebro de rata. Materiales y métodos: Se utilizaron ratas albinas Holtzman mantenidas en un bioterio con temperatura de 22 ± 2° C, humedad entre 50 y 70 % y 12 horas de luz y 12 horas de oscuridad, que recibieron 4,0 mg de hierro elemental/ kg p.c. bajo la forma de hierro hemo o como sulfato ferroso + 10 mg de vitamina C durante siete días, a cuyo término se sacrificaron y se les extrajo sangre, hígado y cerebro. Se hicieron los cortes histológicos que se trataron con hematoxilina-eosina para la observación microscópica y en el suero se midió la capacidad antioxidante. Resultados: Los cerebros de las ratas que recibieron tratamiento con hierro hemo y sulfato ferroso + vitamina C no sufrieron alteraciones significativas, mientras que los cortes histol�...

Objective: To determine the effect exerted by the administration of heme iron and ferrous sulfate with vitamin C in rat liver and brain. Materials and methods: The study used Holtzman albino rats housed in a bioterium with a temperature of 22 ± 2 °C, humidity between 50 and 70 %, and 12 hours of light and 12 hours of darkness. They received elemental iron 4.0 mg/kg b.w. as heme iron or ferrous sulfate + vitamin C 10 mg for seven days, at which time they were sacrificed, and blood, liver and brain were extracted. Histological sections were made and treated with hematoxylin-eosin for microscopic observation, and serum antioxidant capacity was measured. Results: The brains of the rats treated with heme iron and ferrous sulfate + vitamin C did not undergo significant changes, while the histological sections of the livers of the rats treated with heme iron showed a parenchyma without polar ...

We assessed the technique using free radical 2,2-diphenyl-1-picrilhidrazil (DPPH) used to determine the antioxidant capacity of foods and synthetic compounds; for that purpose this free radical has been used in concentrations between 0.037 and 0.2 mM, likewise, to perform this evaluation the catechin and epicatechin standards have been used in a concentration range of 6.67 x 10(-3) and 2.2 x 10(-2) mM. CI(50) values for both standards were dependent on the concentration of DPPH, having observed that the above value increases with increasing concentration of DPPH, for which reason it is suggested that all research use a single concentration of DPPH in the reaction medium.

Objetivo: Determinar el efecto que ejerce la administración de hierro hemo y la de sulfato ferroso con vitamina C en hígado y cerebro de rata. Materiales y métodos: Se utilizaron ratas albinas Holtzman mantenidas en un bioterio con temperatura de 22 ± 2° C, humedad entre 50 y 70 % y 12 horas de luz y 12 horas de oscuridad, que recibieron 4,0 mg de hierro elemental/ kg p.c. bajo la forma de hierro hemo o como sulfato ferroso + 10 mg de vitamina C durante siete días, a cuyo término se sacrificaron y se les extrajo sangre, hígado y cerebro. Se hicieron los cortes histológicos que se trataron con hematoxilina-eosina para la observación microscópica y en el suero se midió la capacidad antioxidante. Resultados: Los cerebros de las ratas que recibieron tratamiento con hierro hemo y sulfato ferroso + vitamina C no sufrieron alteraciones significativas, mientras que los cortes histol�...

Free radicals are compounds characterized by having an unpaired electron in their outer orbit, a condition that makes them highly reactive, i.e., they interact through diffusion-controlled reactions with proteins, lipids and nucleic acids.They have also been referred to as reactive oxygen species (ROS), reactive nitrogen species (RNS) or reactive sulfur species(RSS). In the human organism, they are mainly produced in the mitochondrial electron transport chain, where respiratorycomplexes I and III specifically participate and reduce oxygen by converting it into superoxide anion. Likewise, they canbe formed through a wide variety of enzymatic and non-enzymatic reactions involving substances that are synthesized by cells or are ingested with food and some medicines. Human beings have an antioxidant system which is both enzymaticand non-enzymatic in nature and whose function is to protect th...

Objective: To determine the adherence to heme iron gummy supplementation among children 6 to 8 years of age in the district of Ate, Lima. Materials and methods: A purposive non-probability sampling of 50 children 6 to 8 years of age, of both sexes, registered in the municipality of Ate. The study included children with no anemia, mild anemia and moderate anemia. The hemoglobin level was determined at the beginning and end of the intervention. Each child ingested a heme iron gummy (9 mg elemental iron) for four months. Results: Adherence to heme iron gummies consumption was 100 %; none of the children reported side effects and all of them liked the taste of the gummies. Children with moderate anemia, mild anemia and no anemia had a hemoglobin concentration of 10.48 ± 0.48 g/dL and 11.43 ± 0.38 g/dL, 11.21 ± 0.14 g/dL and 12.17 ± 0.51 g/dL, and 11.68 ± 0.13 g/dL and 12.57 ± 0.53 g/dL...

Objetivo: Determinar la adherencia a la suplementación con gomitas que contienen hierro hemo en niños de 6 a 8 años en el distrito de Ate en Lima. Materiales y métodos: Se realizó un muestreo no probabilístico de tipo intencional de 50 niños de 6 a 8 años de ambos sexos registrados en el municipio de Ate. Se incluyó a niños sin anemia, con anemia leve y anemia moderada. El nivel de hemoglobina se determinó al inicio y al término de la intervención. Cada niño ingirió una gomita con hierro hemo (9 mg de hierro elemental) durante 4 meses. Resultados: La adherencia al consumo fue del 100 %; ninguno de los niños manifestó haber sufrido efectos secundarios y a todos les agradó el sabor de las gomitas. Los niños con anemia moderada tuvieron una concentración de hemoglobina de 10,48 ± 0,48 g/dL al iniciar la intervención y 11,43 ± 0,38 g/dL al finalizar; los niños con ane...

Objetivo: Determinar el efecto que ejerce la vitamina C sobre el sulfato ferroso, principio activo de un jarabe antianémico. Materiales y métodos: La reacción que se produce entre el sulfato ferroso de un jarabe antianémico y la vitamina C se determinó al aplicar la técnica de descomposición de la desoxirribosa, que evalúa la formación de malondialdehído por acción de radicales libres. Resultados: En un medio de ensayo constituido por tampón fosfato 50 mM (pH 7,4), el jarabe antianémico de sulfato ferroso a una concentración 1,080 mM reaccionó con la vitamina C en concentraciones comprendidas entre 5,0 x 10-6 mM y 0,5 mM, generando radicales libres que disminuyen cuando se utiliza una concentración de vitamina C de 5,0 x 10-2 mM mientras que a una concentración 0,5 mM, se eleva. Cuando se usa sulfato ferroso en condiciones similares se aprecia un incremento de la generac...

Objective: To determine the effect of vitamin C on ferrous sulfate, the active ingredient of an antianemic syrup. Materials and methods: The reaction between the ferrous sulfate contained in an antianemic syrup and vitamin C was determined using the decomposition technique of deoxyribose, which evaluates the formation of malondialdehyde by the action of free radicals. Results: In an assay medium consisting of 50 mM phosphate buffer (pH 7.4), the ferrous sulfate antianemic syrup at a concentration of 1.080 mM reacted with vitamin C at concentrations between 5.0 x 10-6 mM and 0.5 mM, generating levels of free radicals that decrease when vitamin C is used at a concentration of 5.0 x 10-2 mM, and increase at a concentration of 0.5 mM. When ferrous sulfate is used under similar conditions, an increase in the generation of free radicals is observed, which reaches a maximum value with vitamin ...

Objetivo: Determinar el efecto que ejerce la vitamina C sobre el sulfato ferroso, principio activo de un jarabe antianémico. Materiales y métodos: La reacción que se produce entre el sulfato ferroso de un jarabe antianémico y la vitamina C se determinó al aplicar la técnica de descomposición de la desoxirribosa, que evalúa la formación de malondialdehído por acción de radicales libres. Resultados: En un medio de ensayo constituido por tampón fosfato 50 mM (pH 7,4), el jarabe antianémico de sulfato ferroso a una concentración 1,080 mM reaccionó con la vitamina C en concentraciones comprendidas entre 5,0 x 10-6 mM y 0,5 mM, generando radicales libres que disminuyen cuando se utiliza una concentración de vitamina C de 5,0 x 10-2 mM mientras que a una concentración 0,5 mM, se eleva. Cuando se usa sulfato ferroso en condiciones similares se aprecia un incremento de la generac...

Objective: To evaluate antidiabetic activity of water and ethanol extracts from Psidium guajava L. leaves in alloxan-induced diabetic rats. Material and Methods: Hyperglycemia was induced in albino rats by administering alloxan monohydrate (100 mg/kg i.p.). Water extract at a dose of 250 mg/kg was administered at a single dose, while ethanol extracts at doses of 250 and 500 mg/kg were also administered at a single dose. Blood glucose was determined two hours after administering the extracts. Results: The addition of water and ethanol extracts of Psidium guajava L. tended to decrease the blood glucose concentration. The water extract at a concentration of 250 mg/kg was found to be more potent than ethanol extracts with the lowest mean glucose concentration of 86.67 mg/dL two hours after it was administered. The effect of ethanol extract was only observed at the concentration of 500 mg/kg ...

Objective: To determine the content of bioactive compounds and antioxidant capacity of the banana passionfruit (Passiflora mollissima) and black cherry (Prunus serotina). Materials and methods: An analytical, experimental, longitudinal and prospective study. The black cherries and banana passionfruits were collected in the Cusco, Moquegua and Arequipa regions. The content of phenols and flavonoids weredetermined using the Folin–Ciocalteu reagent and aluminum chloride method, respectively. The antioxidant activity was evaluated using the ferric reducing ability of plasma (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and thiobarbituric acid reactive substance (TBARS) techniques. Albino rats classified into a negative control group, a positive control group and four experimental groups were used to study the hepatoprotective effect of the fruits. Results: Banana passionfruits from the Cus...