Toxoplasma gondii is the causal agent of toxoplasmosis, a re-emerging and cosmopolitan zoonosis, affecting intermediate and definitive hosts. The domestic cat is the definitive host closer to the human that develops the sexual form of the parasite and it is the producer of oocysts. For this, its presence in the environment is essential in the biologic cycle of T. gondii. Research in cats worldwide is limited principally due by the complexity in the sampling; however, those studies have developed knowledge about taxonomy, morphology, biologic cycle and molecular biology, as well as its geographic distribution, hosts, resistance to environment, transmission patterns, contamination sources and rick factors associated to infection in this species and in other vulnerable species. Besides, studies have been conducted for the understanding of the pathogeny, clinical signs, anatomo-pathological ...

Toxoplasma gondii is a protozoan with a wide range of intermediate and definitive (felines) hosts and causal agent of toxoplasmosis, re-emergent zoonosis of worldwide distribution. The seroprevalence in humans in Cuba is high but the prevalence in the domestic cat in the capital city is unknown. The present study aimed to determine the seroprevalence of T. gondii in Felis catus in Havana, Cuba. A total of 300 serum samples from domestic cats were collected from 15 municipalities from October 2010 until April 2011. Samples were analysed by an ELISA Inhibition assay. Municipalities were grouped in a Central and in a Peripheral zone. The seroprevalence was 70%, without significant differences between zones and municipalities. The results allowed concluding that the seroprevalence of T. gondii in domestic cats in Havana was high, which constitutes a potential risk of infection for all hosts,...

The objective of the study was to characterize a Gumboro disease outbreak in apoultry farm of Havana, Cuba in 2010. A population of 2959 broilers was studied. Breedingmanagement, clinical observations and anatomopathological findings were analyzed.The morbidity, mortality and lethality rates were calculated. Necropsy was done in 40 birds with signs of illness for the histopathological study of the bursa of Fabricius,thymus and spleen. The presence of virus antigens in bursal macerated was conductedby an AC-ELISA. The study showed 5.1% mortality, 8.4% morbidity, and 60.6% lethality.The most frequent clinical sign was general depression (100%) and macroscopic lesionswere hemorrhagic bursitis, edema of the bursa and nephritis (85-90%). Follicular lymphoiddepletion in bursa was found in the histopathology. The AC-ELISA showed that 82.5%of bursas were positive. Vaccination and biosecurity def...

The aim of this study was to determine the seroprevalence of T. gondii in Gallus domesticus in Havana, Cuba. Three hundred serum samples were collected from White Leghorn pullets L33 in growing stage in 2015. Samples represented 100 birds per batch incorporated into the poultry unit from different origins. Samples were evaluated by an ELISA inhibition test and data was analyzed by the Chi-square and Duncan tests to compare proportions of positive birds among batches. A relatively low seroprevalence of T. gondii was found (9.6%), similar to other findings reported internationally. Significant differences (p=0.0001) in the prevalence of T. gondii among the three bird batches were found. It is concluded that the seroprevalence of T. gondii in Gallus domesticus in Havana is low, though this constitutes a risk of infection for susceptible human and animal populations.

The aim of this study was to determine the effect of body weight on other morphometric characters in White Leghorn pullets L33. The study lasted for 6 weeks using 300 pullets (11-16 weeks old) equally distributed in two experimental groups: I: expected body weight (865.6 ± 13.3 g); II: overweighed (920.1 ± 37.3 g). Body weight, tarsus length and distances between pubic bones and from keel to pubis were determined weekly. The Mann-Whitney U test (p<0.05) was used to compare means between groups. Degree of uniformity in body weight in both groups was considered excellent (2.6 and 4.8% for group I and II, respectively). Significant difference between groups was observed in the tarsus length and distance from keel to pubis (p<0.0001), but not on the distance between pubic bones. Also, positive correlation between body weight and other morphometric variables was observed, although onl...

The aim of this study was to conduct a haematological evaluation of dogs and cats in Lima, Peru. A total of 460 blood samples (410 dogs and 50 cats) were collected between December 2017 and May 2018. The smears were stained with Wright and the reticulocytes were stained with Brilliant cresyl blue. Descriptive statistics were performed for quantitative variables in the erythroid, myeloid and lymphoid series, as well as the frequency analysis of qualitative variables between species indicator of type of anaemia and leukaemia, ability of medullary regeneration, abnormal morphological changes, out of physiological range and presence of extra and intracellular microorganisms. The most frequent alterations were normochromic normocytic anaemia (23.2% dog, 10% cat), megaloblastic anaemia (5.1% dog), severe hypochromic microcytic anaemia (4% cat), neutrophilic chronic myeloid leukaemia (7.1% dog,...

Toxoplasma gondii is the causal agent of toxoplasmosis, a re-emerging and cosmopolitan zoonosis, affecting intermediate and definitive hosts. The domestic cat is the definitive host closer to the human that develops the sexual form of the parasite and it is the producer of oocysts. For this, its presence in the environment is essential in the biologic cycle of T. gondii. Research in cats worldwide is limited principally due by the complexity in the sampling; however, those studies have developed knowledge about taxonomy, morphology, biologic cycle and molecular biology, as well as its geographic distribution, hosts, resistance to environment, transmission patterns, contamination sources and rick factors associated to infection in this species and in other vulnerable species. Besides, studies have been conducted for the understanding of the pathogeny, clinical signs, anatomo-pathological ...

Toxoplasma gondii is a protozoan with a wide range of intermediate and definitive (felines) hosts and causal agent of toxoplasmosis, re-emergent zoonosis of worldwide distribution. The seroprevalence in humans in Cuba is high but the prevalence in the domestic cat in the capital city is unknown. The present study aimed to determine the seroprevalence of T. gondii in Felis catus in Havana, Cuba. A total of 300 serum samples from domestic cats were collected from 15 municipalities from October 2010 until April 2011. Samples were analysed by an ELISA Inhibition assay. Municipalities were grouped in a Central and in a Peripheral zone. The seroprevalence was 70%, without significant differences between zones and municipalities. The results allowed concluding that the seroprevalence of T. gondii in domestic cats in Havana was high, which constitutes a potential risk of infection for all hosts,...

El objetivo del estudio fue caracterizar un brote de la enfermedad de Gumboro en una granja avícola de La Habana, Cuba, en 2010. Se estudió una población de 2959 pollos de engorde, analizándose los antecedentes de crianza, observaciones clínicas y hallazgos anatomopatológicos. Se calcularon los índices de morbilidad, mortalidad y letalidad. Se realizó la necropsia a 40 aves con signos clínicos para el estudio histopatológico de la bolsa de Fabricio, timo y bazo y se determinó la presencia de antígenos virales en macerado bursal mediante un AC-ELISA. Se evidenció una mortalidad de 5.1%, morbilidad de 8.4% y letalidad de 60.6%. El signo clínico más frecuente fue la depresión general (100%)y las lesiones más frecuentes fueron bursitis hemorrágica, edema bursal y nefritis (85-90%), habiendo depleción linfoide folicular en los cortes histológicos de bolsa. El 82.5% de las ...

El estudio tuvo como objetivo determinar la seroprevalencia de T. gondii en Gallus domesticus en La Habana, Cuba. Se colectaron 300 muestras de sueros de pollitas White Leghorn L33 en fase de desarrollo en 2015, distribuidas a razón de 100 aves por cada lote incorporado a la unidad avícola desde procedencias diferentes. Las muestras fueron analizadas mediante un ELISA de inhibición y se utilizaron las pruebas Chi-cuadrado y Dócima de Duncan para comparar las proporciones de aves positivas entre los lotes estudiados. Se encontró una relativa baja seroprevalencia de T. gondii (9.6%), similar a otros hallazgos reportados a nivel internacional. Se evidenciaron diferencias significativas (p=0.0001) en la prevalencia de T. gondii entre los tres lotes de aves. Se concluye que la seroprevalencia de T. gondii en Gallus domesticus en La Habana es baja, aunque esta constituye un riesgo de infe...

El objetivo del presente estudio fue determinar el efecto del peso corporal sobre otros caracteres morfométricos en pollitas White Leghorn L33. Se trabajó con 300 pollitas por seis semanas a partir de la semana 11 de edad. La mitad (grupo I) tenían el peso esperado para su edad (865.6 ± 13.3 g) y la otra mitad (grupo II) estaban con sobrepeso (920.1 ± 37.3 g). Se registró semanalmente el peso corporal, largo del tarso, y distancias entre pubis y de quilla a pubis. Se utilizó la prueba U de Mann-Whitney (p<0.05) para comparar las medias entre grupos. La uniformidad en el peso corporal de ambos grupos fue considerada como excelente (2.6 y 4.8% para el grupo I y II, respectivamente). Se observó diferencia significativa entre grupos en el largo del tarso y la distancia de quilla a pubis (p<0.0001), pero no en la distancia entre pubis. Asimismo, se observó correlación positiva...

The aim of this study was to conduct a haematological evaluation of dogs and cats in Lima, Peru. A total of 460 blood samples (410 dogs and 50 cats) were collected between December 2017 and May 2018. The smears were stained with Wright and the reticulocytes were stained with Brilliant cresyl blue. Descriptive statistics were performed for quantitative variables in the erythroid, myeloid and lymphoid series, as well as the frequency analysis of qualitative variables between species indicator of type of anaemia and leukaemia, ability of medullary regeneration, abnormal morphological changes, out of physiological range and presence of extra and intracellular microorganisms. The most frequent alterations were normochromic normocytic anaemia (23.2% dog, 10% cat), megaloblastic anaemia (5.1% dog), severe hypochromic microcytic anaemia (4% cat), neutrophilic chronic myeloid leukaemia (7.1% dog,...

The aim of this study was to describe the morphopathological changes in proventriculus of wild ducks (Anas discors) infested with Tetrameres americana. Necropsy to 89 cadavers of adult ducks from Pinar del Río, Cuba, was performed. Samples of 1 cm2 from 22 proventriculi diagnosed with parasitic proventriculitis were taken for the histopathological study. All proventriculi ducts were collected for parasitological analysis and the lesions were classified as mild and severe according to the amount of glands affected with the nematode and the magnitude of catarrhal exudate. The results showed that 25% (22/89) of the ducks had proventriculitis caused by T. americana where 73% of the cases was considered as mild. Mucosal erosion, degeneration with necrosis and formation of nodules of 1 cm of diameter in the glandular yolk were observed. It is concluded that infestation with T. americana cause...

El estudio tuvo como objetivo describir los cambios morfopatológicos en proventrículo de patos silvestres (Anas discors) infestados con Tetrameres americana. Se realizó la necropsia a 89 cadáveres de patos adultos procedentes de Pinar del Río, Cuba, y se tomó un fragmento de 1 cm2 de 22 proventrículos con diagnóstico clínico de proventriculitis parasitaria para el estudio histopatológico. Los órganos se colectaron para su análisis parasitológico, clasificando las proventriculitis en leve y severa, según la cantidad de glándulas comprometidas con el nematodo y la magnitud del exudado catarral. El 25% (22/89) de las aves presentó proventriculitis catarral con presencia de T. americana, donde el 73% de los casos fue considerado como leve. Se observó erosión de la mucosa, degeneración con necrosis y formación de nódulos de 1 cm de diámetro en la yema glandular. Se concl...

The chronic respiratory disease (CRD) is considered as an infectocontagious disease of multiple etiology that causes great economic losses in poultry farming. The clinical signs and lesions in affected animals can be severe when mixed infections occur. In Cuba this respiratory complex is evident; however, it is not known how the hemathological values vary in affected fowls. The objective of this study was to determine the hemathological values and anatomopathological lesions in CRD affected White Leghorn hens. A total of 34 hens of 32 weeks of age were sampled in an affected flock (17 affected and 17 healthy) and was conducted the clinical evaluation, necropsy, isolation and identification of bacteria and hematological analysis. The frequencies of microorganisms and lesions present, as well as means of hematological variables between ill and healthy birds were compared. The presence of O...

La enfermedad respiratoria crónica (ERC) es considerada como una de las enfermedades infectocontagiosas de etiología múltiple que ocasiona grandes pérdidas económicas en la avicultura. Las manifestaciones clínicas y lesiones pueden ser muy severas cuando ocurren infecciones mixtas. Este complejo respiratorio ha estado presente en Cuba; sin embargo, no se conoce cómo varían los valores hematológicos en aves afectadas. El objetivo del presente estudio fue determinar los valores hematológicos y las lesiones anatomopatológicas en gallinas White Leghorn afectadas por la ERC. Se muestrearon 34 animales de 32 semanas de edad dentro de un foco activo (17 afectados y 17 clínicamente sanos) para la exploración clínica, necropsia, aislamiento e identificación de bacterias, y evaluación hematológica. Se compararon frecuencias de microorganismos y lesiones presentes, así como las me...