The aim of this study was to evaluate the detection capacity of the Multiplex Polymerase Chain Reaction (PCR) method from non-selective pre-enrichment samples (using target sequences of InvA, fliC and prot6E genes) to diagnose Salmonella Typhimurium and Enteritidis and to determine the concordance between the conventional microbiological detection method which consist of non-selective pre-enrichment, selective enrichment, differential agar isolation and biochemical tests. A total of 111 liver samples from guinea pigs with presumptive diagnosis of salmonellosis, collected in Chancay (Lima) and El Mantaro (Junín), Peru were analyzed. Salmonella Typhimurium was detected by multiplex PCR in 54% (60/111) of the samples and by microbiological analysis in 41% (45/111). A substantial concordance was found with a Kappa value of 0.64. The McNemar test showed that the results of both tests were st...

The aim of this study was to evaluate the detection capacity of the Multiplex Polymerase Chain Reaction (PCR) method from non-selective pre-enrichment samples (using target sequences of InvA, fliC and prot6E genes) to diagnose Salmonella Typhimurium and Enteritidis and to determine the concordance between the conventional microbiological detection method which consist of non-selective pre-enrichment, selective enrichment, differential agar isolation and biochemical tests. A total of 111 liver samples from guinea pigs with presumptive diagnosis of salmonellosis, collected in Chancay (Lima) and El Mantaro (Junín), Peru were analyzed. Salmonella Typhimurium was detected by multiplex PCR in 54% (60/111) of the samples and by microbiological analysis in 41% (45/111). A substantial concordance was found with a Kappa value of 0.64. The McNemar test showed that the results of both tests were st...

El estudio del síndrome de burnout en las empresas es crucial por su impacto en la salud y productividad de los empleados, la retención del personal y la cultura organizacional. Este TFM revela que el burnout afecta negativamente el desempeño laboral: disminuye la productividad, provoca agotamiento emocional y falta de motivación, dificulta las relaciones laborales, aumenta el ausentismo y la rotación de personal, y afecta la salud mental y física. La investigación subraya la relación adversa entre el burnout y el desempeño laboral, destacando la importancia de abordar este síndrome para fomentar un ambiente laboral saludable y sostenible, así como garantizar el bienestar y la satisfacción de los empleados.

stephanyoconnor2@gmail.com

Consumers increasingly demand products in their diets that not only provide the nutrients required for a healthy life, they are also preferably those that can complement with beneficial health properties. In recent decades it has been recognized rosehip, Rosa and Rosa canina the rubiginosa as fruits contain many nutraceutical properties. This paper reviews the research papers carried the Rosehip from the point of view of their importance of their consumption and their health benefits; describing the chemical, physical and biochemical characteristics such as content of phenolic compounds, fatty acids, linoleic acid, mineral; and other important also from an industrial point of view, such as antioxidants, pigments, among other compounds. According to the information collected, the Rosehip is a source of micronutrients including vitamin C and lycopene, also delivering a good nutritional val...

Consumers increasingly demand products in their diets that not only provide the nutrients required for a healthy life, they are also preferably those that can complement with beneficial health properties. In recent decades it has been recognized rosehip, Rosa and Rosa canina the rubiginosa as fruits contain many nutraceutical properties. This paper reviews the research papers carried the Rosehip from the point of view of their importance of their consumption and their health benefits; describing the chemical, physical and biochemical characteristics such as content of phenolic compounds, fatty acids, linoleic acid, mineral; and other important also from an industrial point of view, such as antioxidants, pigments, among other compounds. According to the information collected, the Rosehip is a source of micronutrients including vitamin C and lycopene, also delivering a good nutritional val...

The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from guinea pigs.

El objetivo del presente estudio fue identificar mediante PCR múltiple la posible existencia de los serovares Salmonella Typhimurium y Enteritidis en 25 cepas de Salmonella spp previamente aisladas de cuyes e identificadas por sus características metabólicas. Mediante el análisis molecular se identificaron todas las cepas como Salmonella Typhimurium, evidenciando la amplificación de los cebadores específicos para los genes invA y fliC pertenecientes al género Salmonella y Salmonella Typhimurium, respectivamente. El presente estudio permitió establecer una metodología rápida para la identificación molecular de Salmonella Typhimurium y Enteritidis aislados de cuyes.

The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmo...

The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmo...

This study aimed to isolate and identify arbuscular mycorrhizal fungi in melina (Gmelina arborea Roxb) plantations. Two melina plantations of 1 and 3 years old located in the central zone of the humid Ecuadorian tropics, province of Los Ríos, Valencia were sampled. Soil and root samples were collected in the dry season between June and December 2015. The results showed the presence of the arbuscular mycorrhizal fungi genera Glomus and Gigaspora. The genus with the greatest representativeness in quantity of spores per gram of soil was Glomus. The highest percentage of mycorrhizal colonization occurred in the roots of 1-year-old trees.

El objetivo de esta investigación fue aislar e identificar hongos formadores de micorriza arbuscular en plantaciones de melina (Gmelina arborea Roxb). Se muestrearon dos plantaciones de melina de 1 y 3 años de edad, ubicadas en la zona central del trópico húmedo ecuatoriano, provincia de Los Ríos, cantón Valencia. Las muestras de suelo y raíces se recolectaron en la época seca entre junio y diciembre de 2015. Los resultados mostraron la presencia de los géneros de hongos micorrízicos arbusculares Glomus y Gigaspora. El género con mayor representatividad en cantidad de esporas por gramo de suelo fue Glomus. El mayor porcentaje de colonización micorrízica se presentó en las raíces de los árboles de 1 año de edad.