El estudio tuvo como objetivo identificar bacterias anaeróbicas aisladas del compartimento 1 del tracto digestivo de las alpacas. Se obtuvieron 4 aislamientos del licor (LC1) y 9 de la pared del compartimento (PC1) de los tractos digestivos. Los aislamientos de LC1 se cultivaron en agar Anaeróbico de Brewer (BA), y los aislamientos de PC1 en BA suplementado con L-cisteína. Los aislamientos anaeróbicos fueron sometidos a identificación mediante observación microscópica y pruebas bioquímicas, seguidas de la extracción de ADN bacteriano total. La amplificación se realizó utilizando cebadores 27F-1492R en el gen 16S ARNr, y se secuenció utilizando el método Sanger con un analizador de ADN ABI PRISM 3730XL. El análisis bioinformático reveló que las cepas correspondientes a la especie de LC1 eran cuatro Streptococcus equinus y de PC1 eran nueve Streptococcus vicugnae. En el an�...

The concentrations of total proteins, glucose, cholesterol, triglycerides, phospholipids, calcium, phosphorus, magnesium, alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT) and 17-β-estradiol in the follicular fluid were determined in non-pregnant Huacaya alpacas in reproductive age according to the development of the ovarian follicle (size). In total, 60 slaughtered alpacas in the local slaughterhouse were used. Samples of small (<4 mm), medium (5-7 mm) and large (>7 mm) follicles were taken (n = 20 by size). The diameter of the follicles was measured with a vernier and the follicular fluid was aspirated. The samples were analysed in the Biochemistry Laboratory of the Universidad Nacional del Altiplano (Puno, Peru) using colorimetric and spectrophotometric techniques. The concentration results for small, medium and large follicles were: total pro...

The concentrations of total proteins, glucose, cholesterol, triglycerides, phospholipids, calcium, phosphorus, magnesium, alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT) and 17-β-estradiol in the follicular fluid were determined in non-pregnant Huacaya alpacas in reproductive age according to the development of the ovarian follicle (size). In total, 60 slaughtered alpacas in the local slaughterhouse were used. Samples of small (<4 mm), medium (5-7 mm) and large (>7 mm) follicles were taken (n = 20 by size). The diameter of the follicles was measured with a vernier and the follicular fluid was aspirated. The samples were analysed in the Biochemistry Laboratory of the Universidad Nacional del Altiplano (Puno, Peru) using colorimetric and spectrophotometric techniques. The concentration results for small, medium and large follicles were: total pro...

Alpacas, important genetic resources in the Andean region of Peru, are vulnerable to diarrhea caused by pathogenic parasites such as Eimeria lamae and Giardia sp., which can be fatal, especially in neonates, due to their physiological immaturity and limited adaptability. The study investigated the diversity and abundance of intestinal fungi and protists in alpacas infected with Eimeria lamae and Giardia sp. compared to healthy alpacas. A total of 19 alpacas, aged between one and two months, were included. They were divided into two groups, one with pathological conditions (nine) and the other healthy (ten). Parasitological analyses for the detection of parasites and subsequent molecular analysis were performed on the collected fecal samples. The results revealed a greater diversity and abundance of protists in infected alpacas in comparison with healthy alpacas, while the fungal composit...

This study aimed to determine the impact of age on the fecal microbiota in the genetic nucleus of cattle, with a focus on microbial richness, composition, functional diversity, and correlations with blood parameters. Fecal and blood samples from 21 cattle were analyzed using 16S rRNA gene sequencing. Older cattle exhibited greater bacterial diversity and abundance, with significant changes in alpha diversity indices (p < 0.05). Beta diversity analysis revealed significant variations in microbial composition between age groups and the interaction of age and sex (p < 0.05). Correlations between alpha diversity, community composition, and hematological values highlighted the influence of microbiota on bovine health. Beneficial butyrate-producing bacteria, such as Ruminococcaceae, were more abundant in older cattle, suggesting a role in gut health. Functional diversity analysis indicated tha...

This study investigates gender-based differences in the gut microbiota of Simmental cattle, focusing on bacterial, archaeal, and fungal communities. Fecal samples were collected and analyzed using high-throughput sequencing, with taxonomic classification performed through the SILVA and UNITE databases. Alpha and beta diversity metrics were assessed, revealing significant differences in the diversity and composition of archaeal communities between males and females. Notably, females exhibited higher alpha diversity in archaea, while beta diversity analyses indicated distinct clustering of bacterial and archaeal communities by gender. The study also identified correlations between specific microbial taxa and hematological parameters, with Treponema and Methanosphaera showing gender-specific associations that may influence cattle health and productivity. These findings highlight the importa...

Diarrhea in alpaca crias significantly impacts livestock health in high-altitude regions, with Escherichia coli as a common pathogen. This study analyzed 10 E. coli isolates from diarrheic and healthy alpacas using whole-genome sequencing to assess genetic diversity, virulence factors, and antibiotic resistance. Predominant sequence types (ST73, ST29), serotypes (O22:H1, O109:H11), and phylogroups (B2, B1, A) were identified. Virulence profiling revealed ExPEC-like and EPEC pathotypes, while resistance genes for β-lactams (blaEC-15), fosfomycin (glpT_E448K), and colistin (pmrB) were prevalent. These findings highlight the need for genomic surveillance and antimicrobial stewardship to manage E. coli infections in alpacas and reduce public health risks.