Esta investigación tuvo como objetivos aislar e identificar bacterias cultivables del sistema radicular de Rhizophora mangle para lo cual se tomó 1 gr muestra y se colocó en 1ml de solución salina para luego ser incubadas en el medio Bushnell Haas por 48 horas para ser sembrada por agotamiento en TSA. Para la identificación molecular de las bacterias se realizó la amplificación de los genes 16S ARNr y Benceno Di-oxigenasa. Se obtuvo como resultados de esta investigación el aislamiento y conservación total de 78 cepas bacterianas procedente del canal de marea Puerto Pizarro (Isla Bajo Grande y Puerto Rico) y El Bendito (Zona de amortiguamiento), además se aislaron 37 cepas bacterianas de Puerto Hualtaco (Ecuador). Se utilizaron los medios de cultivo Agar Tripticasa Soya y Bushnell-Haas. Las características morfológicas de las cepas aisladas fueron color: blanco, blanco transpa...

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...