The aim of this study was determine the frequency of seropositivity to a Ehrlichia canis in human subjects by indirect inmunofluorescent test (IFI), hematological changes and its association with risk factors such as age, gender, level of contact with dogs, and tick exposure. Ninety-one samples were collected from patients without reference to gender, age and socioeconomic status, but whose dogs had history of ehrlichiosis within six months to the sampling. The study was conducted between February and May 2010. Hematology results revealed absence of positive cases and 15.4% of suspected cases (presence of inclusion bodies in mononuclear cells and absence of thrombocytopenia). Frequency of IFI seropositives was 14.3%. No statistical difference was found due to the effect of the variables under evaluation. The results confirm exposure to E. canis in dog owners with previous record of ehrli...

El presente estudio tuvo por objetivo determinar la seropositividad a Ehrlichia canis mediante la prueba de inmunofluorescencia indirecta (IFI) en pacientes humanos, sus parámetros hematológicos y su asociación con las variables edad, sexo, nivel de contacto con perros y antecedentes de exposición a garrapatas. Se evaluaron 91 sujetos sin distinción de género, edad o condición socioeconómica cuyos perros tenían historia de erlichiosis dentro de los seis meses previos al muestreo. El muestreo se realizó entre febrero y mayo de 2010. La evaluación hematológica mostró ausencia de pacientes positivos y 15.4% de pacientes sospechosos (presencia de corpúsculos de inclusión en células mononucleares y ausencia de trombocitopenia). La frecuencia de pacientes seropositivos mediante IFI fue de 14.3%. No se encontró diferencia estadística por efecto de las variables en estudio. Los...

This study aimed to determine the presence ofAnaplasma platys in domestic dogs of Metropolitan Lima with clinic signs consistent with anaplasmosis by identifying inclusion bodies in platelets and through the Hemi-Nested PCR technique using peripheral blood samples. For this purpose, 144 samples were collected between January to December 2012. The results showed that 29.2% (42/144) were positive (thrombocytopenic individuals and presence ofinclusion bodies in platelets) and 12.5% (18/144) were suspicious (nonthrombocytopenic individuals and presence of inclusion bodies in platelets) by hematologic identification. Moreover, 1.4% (2/144) resulted positive to the Hemi-Nested PCR test. These results confirm the presence ofA. platys in domestic dogs in the country

This study aimed to determine the presence ofAnaplasma platys in domestic dogs of Metropolitan Lima with clinic signs consistent with anaplasmosis by identifying inclusion bodies in platelets and through the Hemi-Nested PCR technique using peripheral blood samples. For this purpose, 144 samples were collected between January to December 2012. The results showed that 29.2% (42/144) were positive (thrombocytopenic individuals and presence ofinclusion bodies in platelets) and 12.5% (18/144) were suspicious (nonthrombocytopenic individuals and presence of inclusion bodies in platelets) by hematologic identification. Moreover, 1.4% (2/144) resulted positive to the Hemi-Nested PCR test. These results confirm the presence ofA. platys in domestic dogs in the country

The aim of this study was to determine the presence of canine anaplasmosis by haematological findings (evidence of inclusion bodies or morulae in blood smear plus thrombocytopenia) and the presence of antibodies against Anaplasma spp in dogs with a history of ticks from the Chiclayo district (Lambayeque, Peru). Blood samples (n=88) were collected from apparently healthy dogs with a history of ticks. The haematological findings showed that 2.3% (2/88) of dogs were positive (with inclusion bodies or morulae plus thrombocytopenia) being compatible with A. platys. In addition, 22.7% (20/88) seropositivity against Anaplasma spp and a multiple seropositivity of 21.6% (19/88) with Ehrlichia spp were detected by the SNAP 4Dx Plus Test. Positive and seropositive dogs showed thrombocytopenia and, to a lesser extent, anaemia, leucocytosis and leukopenia. The results showed presence of subclinical c...

The aim of this study was to determine the presence of canine anaplasmosis by haematological findings (evidence of inclusion bodies or morulae in blood smear plus thrombocytopenia) and the presence of antibodies against Anaplasma spp in dogs with a history of ticks from the Chiclayo district (Lambayeque, Peru). Blood samples (n=88) were collected from apparently healthy dogs with a history of ticks. The haematological findings showed that 2.3% (2/88) of dogs were positive (with inclusion bodies or morulae plus thrombocytopenia) being compatible with A. platys. In addition, 22.7% (20/88) seropositivity against Anaplasma spp and a multiple seropositivity of 21.6% (19/88) with Ehrlichia spp were detected by the SNAP 4Dx Plus Test. Positive and seropositive dogs showed thrombocytopenia and, to a lesser extent, anaemia, leucocytosis and leukopenia. The results showed presence of subclinical c...