The aim of the present study is to obtain a protein concentrate from fresh artichoke heart and to determine the amount and quality of protein present. For this reason we employed different extractions in order to obtain acidic, aqueous and alcoholic concentrates with three different alcoholic values, obtaining 13,41g/100g yield of eatable protein with the 86% alcoholic extract, deterrnined by Kjeldahl method, The extracted samples were analyzed by PAGE - SDS electrophoresis, Laemmli method using a 12,5% polyacrilamide concentration, and we found it contained a reduced number of protein bands (between 8 and 10), being the most abundant the ones with Molecular Mass of 24 000, 29 000 and 36 000 Daltons,The amino acid content of lyophilized artichoke heart in the 86% alcoholic concentrate was deterrnined by HPLC, finding that the limiting amino acids were lysine and leucine, being valine and...

The aim of the present study is to obtain a protein concentrate from fresh artichoke heart and to determine the amount and quality of protein present. For this reason we employed different extractions in order to obtain acidic, aqueous and alcoholic concentrates with three different alcoholic values, obtaining 13,41g/100g yield of eatable protein with the 86% alcoholic extract, deterrnined by Kjeldahl method, The extracted samples were analyzed by PAGE - SDS electrophoresis, Laemmli method using a 12,5% polyacrilamide concentration, and we found it contained a reduced number of protein bands (between 8 and 10), being the most abundant the ones with Molecular Mass of 24 000, 29 000 and 36 000 Daltons,The amino acid content of lyophilized artichoke heart in the 86% alcoholic concentrate was deterrnined by HPLC, finding that the limiting amino acids were lysine and leucine, being valine and...

The feasibility of untreated bovine pericardium xenograft as biomaterial for the lining of the small intestine anastomosis in horses to avoid dehiscences, leakage, fistulas and formation of adhesions in the postoperative period was investigated. Seven horses were submitted to end-to-end jejunal anastomosis coated with untreated bovine pericardial xenograft and a control group of seven horses submitted to end-to-end jejunal anastomosis only. Postoperative pain, cellular components and organoleptic characteristics of the peritoneal fluid were evaluated. It was found that the bovine pericardium xenograft coating does not generate significant differences in the quantification of pain (postoperative colic) nor in the total nucleated cell count of the peritoneal fluid nor does it influence the alterations of the color, turbidity and smell of the peritoneal fluid, demonstrating that untreated b...

The evaluation of the untreated bovine pericardium xenograft as a biomaterial for the lining of the small intestine anastomosis in equines was conducted. Seven horses with ages between 3 and 10 years were operated on. Two of them were randomly selected, doing a laparotomy to one of them at 15 days and the other at 100 days post-surgery. In the macroscopic and histopathological studies of the cicatricial area of the end-to-end anastomosis it was evidenced that the xenograft does not contract or move from the line of suture, guaranteeing the process of cicatricial remodeling and avoiding complications such as peritonitis, stenosis, abscesses, anastomotic leakage and adhesions. It is concluded that the untreated bovine pericardium xenograft is viable and effective as a jejunal enterectomy lining, showing good adaptability as a natural microenvironment for progenitor cells responsible for ci...

Se investigó la viabilidad del xenoinjerto de pericardio bovino no tratado como biomaterial para el revestimiento de la anastomosis de intestino delgado en equinos a fin de evitar dehiscencias, fugas, fístulas y formación de adherencias en el posoperatorio. Se trabajó con siete equinos sometidos a anastomosis término-terminal yeyunal revestida con xenoinjerto de pericardio bovino no tratado y un grupo control de siete equinos sometidos solamente a anastomosis término-terminal yeyunal. Se evaluó el dolor postoperatorio, los componentes celulares y las características organolépticas del líquido peritoneal. Se encontró que el revestimiento con xenoinjerto de pericardio bovino no genera diferencias significativas en la cuantificación del dolor (cólico posoperatorio) ni en el recuento total de células nucleadas del líquido peritoneal, ni influye en las alteraciones del color, t...

Se hizo la evaluación del xenoinjerto de pericardio bovino no tratado como biomaterial para el revestimiento de la anastomosis de intestino delgado en equinos. Se practicó la cirugía a siete equinos con edades entre 3 y 10 años. Se seleccionaron al azar a dos de ellos, haciendo una laparatomía a uno de ellos a los 15 días y al otro a los 100 días del posoperatorio. En los estudios macroscópicos e histopatológicos de la zona cicatricial de la anastomosis término-terminal se evidenció que el xenoinjerto no se contrae ni se desplaza de la línea de sutura, garantizando el proceso de remodelación cicatricial y evitando complicaciones como peritonitis, estenosis, abscesos, fugas anastomóticas y adherencias. Se concluye que el xenoinjerto de pericardio bovino no tratado es viable y efectivo, como revestimiento de la enterectomía yeyunal; demostrando buena adaptabilidad como micro...

Se reporta el caso de un equino hembra de raza Pura Sangre de Carrera de tres años con síndrome abdominal agudo relacionado a una hernia umbilical irreductible, inflamada y tumefacta, que fue derivada a cirugía. Durante el procedimiento quirúrgico se detectó una adherencia necrótica en la parte antimesentérica de un segmento del íleon. Con el fin de extraer la porción afectada se practicó una enterotomía, la cual fue reforzada mediante la transposición del ligamento ileocecal para evitar una estenosis o el posible colapso de la línea anastomótica. Los resultados de la cirugía fueron satisfactorios y el paciente evolucionó favorablemente.

The aim of this study was to evaluate the productive and reproductive performance of cows submitted to an induced lactation protocol in a commercial dairy farm in Lima, Perú between 2015 and 2017. A total of 98 records of complete induced lactation and 191 records of normal lactations, both primiparous and multiparous were studied. Total production, corrected production and lactation length, as well as the conception rate and the number of services per conception were evaluated. The average total and corrected milk production were 7546 and 6641 kg, respectively, and 367 days of lactation length for induced lactation cows, while for normal lactating cows, values of 10129.8 kg, 8432.1 kg and 408.9 days were obtained, respectively (p<0.05). Likewise, induced lactation cows obtained 58% of pregnancy with 3.9 services/conception compared to control cows with 72.3% (p<0.05) and 3.6 serv...

The case of a three-year-old Thoroughbred female horse with acute abdominal syndrome related to an irreducible, swollen and swollen umbilical hernia, which was referred to surgery is reported. During the surgical procedure a necrotic adhesion was detected in the antimesenteric part of a segment of the ileum. In order to remove the affected portion, an enterotomy was performed, which was reinforced by transposition of the ileocecal ligament to avoid stenosis or the possible collapse of the anastomotic line. The results of the surgery were satisfactory, and the patient evolved favourably.

The aim of this study was to evaluate the productive and reproductive performance of cows submitted to an induced lactation protocol in a commercial dairy farm in Lima, Perú between 2015 and 2017. A total of 98 records of complete induced lactation and 191 records of normal lactations, both primiparous and multiparous were studied. Total production, corrected production and lactation length, as well as the conception rate and the number of services per conception were evaluated. The average total and corrected milk production were 7546 and 6641 kg, respectively, and 367 days of lactation length for induced lactation cows, while for normal lactating cows, values of 10129.8 kg, 8432.1 kg and 408.9 days were obtained, respectively (p<0.05). Likewise, induced lactation cows obtained 58% of pregnancy with 3.9 services/conception compared to control cows with 72.3% (p<0.05) and 3.6 serv...

This work was supported by CONCYTEC-FONDECYT (Peru), contract N° 159-2020-FONDECYT and the Pontifical Catholic University of Peru.

The aim of this study was to evaluate the phenotypic characteristics of the Creole cattle of the Huascarán National Park (PNH), Ancash, Peru through zoometric measurements and indexes of productive interest. The study was conducted in a population of Creole cattle in the Llanganuco zone of the PNH. Data were collected from 32 cows bovines. The zoometric measurements were head width (AC), head length (LC), chest girth (PT), body length (LCU), height withers (ALC) and height at the rump (ALG), anterior rump width (AAG), rear rump with (APG) and rump length (LG). Indexes related to meat ability were calculated: transversal pelvic index (IPT) and longitudinal pelvic index (IPL), and ethnological indexes: anamorphosis index (AI), corporal index (IC), pelvic index (PI) and proportionality index (IPR). The data were processed with the statistical program R. The colour and pattern of the coat w...

The aim of this study was to evaluate the phenotypic characteristics of the Creole cattle of the Huascarán National Park (PNH), Ancash, Peru through zoometric measurements and indexes of productive interest. The study was conducted in a population of Creole cattle in the Llanganuco zone of the PNH. Data were collected from 32 cows bovines. The zoometric measurements were head width (AC), head length (LC), chest girth (PT), body length (LCU), height withers (ALC) and height at the rump (ALG), anterior rump width (AAG), rear rump with (APG) and rump length (LG). Indexes related to meat ability were calculated: transversal pelvic index (IPT) and longitudinal pelvic index (IPL), and ethnological indexes: anamorphosis index (AI), corporal index (IC), pelvic index (PI) and proportionality index (IPR). The data were processed with the statistical program R. The colour and pattern of the coat w...

Postoperative complications after intestinal anastomosis remain the main concern in abdominal surgery in horses. The aim of this study was to describe an end-to-end jejunal anastomosis with a mesenteric flap covering technique and its macroscopic and postoperative histological characteristics. This technique is based on a reinforcement to the conventional surgery performed with a simple interrupted suture pattern to prevent postoperative complications. Five previously premedicated horses with xylazine 0.5 mg/kg, acepromazine 0.03 mg/kg and tramadol 2 mg/kg were induced to general anesthesia with diazepam 0.25 mg/kg and ketamine 2.2 mg/kg. General anesthesia was maintained with a minimum alveolar concentration of 1.4 ± 0.2% of isoflurane. A midline abdominal wall approach was made. A 10 cm jejunal segment was selected for resection and end-to-end anastomosis. A mesenteric flap large enou...