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https://purl.org/pe-repo/ocde/ford#3.01.05
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Perú
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https://purl.org/pe-repo/ocde/ford#4.03.01
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ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades
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1
artículo
Publicado 2021
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The discovery of antibiotics in the 19th century allowed to treat infectious diseases that to date were incurable. However, nowadays, the number of resistant bacteria has grown remarkably, placing the problem of bacterial resistance as one of the main causes of mortalities worldwide. This work addresses the origin of this phenomenon and the possible solutions that are proposed to minimize its effects.
2
artículo
Publicado 2021
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The discovery of antibiotics in the 19th century allowed to treat infectious diseases that to date were incurable. However, nowadays, the number of resistant bacteria has grown remarkably, placing the problem of bacterial resistance as one of the main causes of mortalities worldwide. This work addresses the origin of this phenomenon and the possible solutions that are proposed to minimize its effects.
3
artículo
Objective: To evaluate the prevalence of bacterial vaginosis in women of a rural population with vaginal discharge. Design: Prospective, transversal, analytical study. Material and Methods: Vaginal secretion samples were taken from 100 patients with vaginal discharge, and microbiologic analysis and Amsel‘s criteria for bacterial vaginosis diagnosis were applied. Results: Bacterial vaginosis was found in 33% of patients that fulfilled at least 3 of the 4 Amsel‘s criteria, candidiasis in 15%, trichomoniasis in 4% and mixed culture in 5%. Clue cells and the amine test allowed us to identify the largest percentage of patients with bacterial vaginosis; likewise, the presence of clue cells alone, clue cells + odor and odor + pH had high positive and negative predictive value. Vaginal discharge sensitivity is low. Conclusions: Bacterial vaginosis is the most common vaginal infection among r...
4
artículo
Objective: To evaluate the prevalence of bacterial vaginosis in women of a rural population with vaginal discharge. Design: Prospective, transversal, analytical study. Material and Methods: Vaginal secretion samples were taken from 100 patients with vaginal discharge, and microbiologic analysis and Amsel‘s criteria for bacterial vaginosis diagnosis were applied. Results: Bacterial vaginosis was found in 33% of patients that fulfilled at least 3 of the 4 Amsel‘s criteria, candidiasis in 15%, trichomoniasis in 4% and mixed culture in 5%. Clue cells and the amine test allowed us to identify the largest percentage of patients with bacterial vaginosis; likewise, the presence of clue cells alone, clue cells + odor and odor + pH had high positive and negative predictive value. Vaginal discharge sensitivity is low. Conclusions: Bacterial vaginosis is the most common vaginal infection among r...
5
artículo
Publicado 2019
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In the present work the Lal method in it kinetic chromogenic variant for the determination of bacterial endotoxins in the injectable Cefepime 1 g is evaluated. The objective of this variant consists in the control of the color development through the time and it uses a standard curve represented by the reaction time as function of the known concentration of endotoxins. The characterization of the product was carried out. The selection of the dilution of work was preliminarily evaluated in the inhibition and rehearsal assay. The elected dilution was validated in three serial lots of the product and it was observed, in all the cases, recovered percent framed inside the established limits. It was demonstrated that the standardization and validation of the method allowits application as routine assay for the determination of bacterial endotoxins as a part of the quality control for this prod...
6
artículo
Publicado 2019
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In the present work the Lal method in it kinetic chromogenic variant for the determination of bacterial endotoxins in the injectable Cefepime 1 g is evaluated. The objective of this variant consists in the control of the color development through the time and it uses a standard curve represented by the reaction time as function of the known concentration of endotoxins. The characterization of the product was carried out. The selection of the dilution of work was preliminarily evaluated in the inhibition and rehearsal assay. The elected dilution was validated in three serial lots of the product and it was observed, in all the cases, recovered percent framed inside the established limits. It was demonstrated that the standardization and validation of the method allowits application as routine assay for the determination of bacterial endotoxins as a part of the quality control for this prod...
7
artículo
Publicado 2019
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In the present work the Lal method in it kinetic chromogenic variant for the determination of bacterial endotoxins in the injectable Cefepime 1 g is evaluated. The objective of this variant consists in the control of the color development through the time and it uses a standard curve represented by the reaction time as function of the known concentration of endotoxins. The characterization of the product was carried out. The selection of the dilution of work was preliminarily evaluated in the inhibition and rehearsal assay. The elected dilution was validated in three serial lots of the product and it was observed, in all the cases, recovered percent framed inside the established limits. It was demonstrated that the standardization and validation of the method allowits application as routine assay for the determination of bacterial endotoxins as a part of the quality control for this prod...
8
artículo
Gross lesions, histopathology and microbiological analysis of samples from 27weaned alpaca tuis from southern Peru that died from acute pneumonia are described. In situ viral antigens [Parainfluenza 3 (PI3), bovine respiratory syncytial (BRSB) and bovine adenovirus type 3 (BAdV-3)] were searched in 15 of the 27 samples by direct immunofluorescent test and bacteriology cultures were performed on all 27 samples. The gross alterations corresponded mostly to extensive multilobar pneumonia (11/27) associated with fibrinous pleuritis (8/27), and congestion and pulmonary edema (8/27). With the exception of peribronquial lymphocitic aggregates observed in one case, the histopathological changes were similar to those described in the literature for acute pneumonia in neonatal cases. In 9 out of 15 pneumonic tissues, 14 viral antigens were detected (5 as single, 3 dual and 1 triple infections). BR...
9
artículo
Gross lesions, histopathology and microbiological analysis of samples from 27weaned alpaca tuis from southern Peru that died from acute pneumonia are described. In situ viral antigens [Parainfluenza 3 (PI3), bovine respiratory syncytial (BRSB) and bovine adenovirus type 3 (BAdV-3)] were searched in 15 of the 27 samples by direct immunofluorescent test and bacteriology cultures were performed on all 27 samples. The gross alterations corresponded mostly to extensive multilobar pneumonia (11/27) associated with fibrinous pleuritis (8/27), and congestion and pulmonary edema (8/27). With the exception of peribronquial lymphocitic aggregates observed in one case, the histopathological changes were similar to those described in the literature for acute pneumonia in neonatal cases. In 9 out of 15 pneumonic tissues, 14 viral antigens were detected (5 as single, 3 dual and 1 triple infections). BR...
10
artículo
Publicado 2003
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This work compare the efficiency of water treatment from Rimac river. Samples from different phases of water treatment (decanted, filtered and chlorinated) were compared with not treated samples. Coliphages quantification was made by Simple Layer and Membrane Filter methods. Most Probable Number (NMP) and Membrane Filter methods were used to assessment of Total Coliforms, Coliforms Thermotolerants and Heterotrophic Bacteria. The standard strain of E. coli C ATCC 13706 was used as coliphages host. The averages of quantified coliphages were of 2267,25 UFP/100 mL in samples with out treatment; 2,11, 2,04 and 1,07 UFP/100 mL in the water samples from decanted, filtered and chlorinated treatment respectively. Total Coliforms and Coliforms Thermotolerants were not detected in samples of chlorinated water. The correlation between coliphages and Total Coliforms in samples of decanted and filtere...
11
artículo
Neonatal sepsis is an important cause of mortality worldwide, with a heterogeneous clinical presentation that makes early diagnosis difficult. In Peru, it represents the main cause of death in infants under one year of age, with coagulase-negative Staphylococcus being identified as the most frequent causal pathogen. However, microbiological characterization and analysis of bacterial resistance in the Peruvian neonatal population is still limited. The objective was to determine the microbiological characteristics and resistance profile of microorganisms causing neonatal sepsis in Peru. The study was carried out following the PRISMA Guidelines and the protocol was registered in PROSPERO (International prospective register of systematic reviews; CRD42024506552). PubMed/MEDLINE, LILACS, SciELO, Scopus and Web of Science were searched systematically from their inception until February 16, 202...
12
artículo
Role of Eisenia foetida in the degradation of profenofos in presence of native bacterial communities
Publicado 2020
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The use of profenofos (PFF) increased due to its e ectiveness against pests resistant to other organophosphates (OP). Its presence in the environment could produce acute or chronic poisoning for people who use it for daily agricultural activities as well as for people who get in contact with polluted soil, water, air or food. In this context, proposing alternatives to accelerate OP degradation processes is important. Therefore, the objective of the present study was to evaluate for 28 days the degradation process of PFF in soils with 50 mg PFF/kg (pH = 7.20 0.31) using Eisenia foetida (EF) and native bacteria (NB). The “control group”showed a PFF degradation of 52.95 1.69% in sterilized soils. NB achieved a degradation of 63.60 3.27%, EF degraded PFF by 72.65 1.92% and the combination of EF and NB, 79.21 1.79%. Bacteria with potential PFF degradation capacity were isolated and identi...
13
artículo
Publicado 2019
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The incidence of bacterial infections in shrimp hepatopancreas cultivated in the Gulf of Nicoya, Costa Rica, presented a high impact on yields in 2016-2017, affecting the survival of the animals and shrimp farm production. In addition, important incidences of Baculovirus penaei, detected in post-larvae used in part of the shrimp ponds, were reported. An Odds Ratio analysis was performed in an observational case-control study to determine the possible participation of this virus as a risk factor for subsequent bacterioses. The study demonstrates the active participation of Baculovirus penaei as an important risk factor. Survival data are presented by ponds infected by the virus vs free ponds.
14
artículo
Publicado 2019
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The incidence of bacterial infections in shrimp hepatopancreas cultivated in the Gulf of Nicoya, Costa Rica, presented a high impact on yields in 2016-2017, affecting the survival of the animals and shrimp farm production. In addition, important incidences of Baculovirus penaei, detected in post-larvae used in part of the shrimp ponds, were reported. An Odds Ratio analysis was performed in an observational case-control study to determine the possible participation of this virus as a risk factor for subsequent bacterioses. The study demonstrates the active participation of Baculovirus penaei as an important risk factor. Survival data are presented by ponds infected by the virus vs free ponds.
15
artículo
Publicado 2020
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Persistent bacteria are the transient variants of a genetically homogeneous population generated by exposure to stress as in antibiotic treatment. They are an epigenetic phenomenon or a non-inherited phenotype, which may be considered as the first line of defense before developing antimicrobial resistance. Despite their discovery more than 70 years ago, their definition, mechanisms of formation, classification and morphologies of clinical implication are still current research topics. In the present research, we describe the relationship between chronic persistent infections and the formation of biofilms as important factors in recurrences, relapses and greater virulence in infections. Likewise, a brief review of the various mechanisms involved in bacterial persistence, their ineffective elimination due to antibiotic tolerance, and possible treatment strategies is provided. Overall, it i...
16
artículo
Publicado 2020
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The study aimed to evaluate the presence of bacteria (total and thermotolerant coliforms) in the treatment of water using Class C Ultraviolet Radiation (UV-C), the research was carried out in the Quality Control Laboratory of the Treatment Plant of Potable Water of the EPS, EMSA Puno, because current regulations require that total and thermotolerant coliform readings in drinking water should be 0 CFU / 100 ml, with UV-C radiation being an alternative for the decrease in Chlorine use. For what was experimented with a dose of 0,00176 W/cm2/s of UV-C radiation with a dominant wavelength of 254 nm, the experimental water samples were subjected to 1, 2, 3, and 4 seconds of exposure to the mentioned radiation. For the determination of the bacterial presence in water (total and thermotolerant coliforms), the Membrane Filter methodology was chosen, the results obtained fo...
17
artículo
Publicado 2020
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The study aimed to evaluate the presence of bacteria (total and thermotolerant coliforms) in the treatment of water using Class C Ultraviolet Radiation (UV-C), the research was carried out in the Quality Control Laboratory of the Treatment Plant of Potable Water of the EPS, EMSA Puno, because current regulations require that total and thermotolerant coliform readings in drinking water should be 0 CFU / 100 ml, with UV-C radiation being an alternative for the decrease in Chlorine use. For what was experimented with a dose of 0,00176 W/cm2/s of UV-C radiation with a dominant wavelength of 254 nm, the experimental water samples were subjected to 1, 2, 3, and 4 seconds of exposure to the mentioned radiation. For the determination of the bacterial presence in water (total and thermotolerant coliforms), the Membrane Filter methodology was chosen, the results obtained fo...
18
artículo
Publicado 2020
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The study aimed to evaluate the presence of bacteria (total and thermotolerant coliforms) in the treatment of water using Class C Ultraviolet Radiation (UV-C), the research was carried out in the Quality Control Laboratory of the Treatment Plant of Potable Water of the EPS, EMSA Puno, because current regulations require that total and thermotolerant coliform readings in drinking water should be 0 CFU / 100 ml, with UV-C radiation being an alternative for the decrease in Chlorine use. For what was experimented with a dose of 0,00176 W/cm2/s of UV-C radiation with a dominant wavelength of 254 nm, the experimental water samples were subjected to 1, 2, 3, and 4 seconds of exposure to the mentioned radiation. For the determination of the bacterial presence in water (total and thermotolerant coliforms), the Membrane Filter methodology was chosen, the results obtained fo...
19
artículo
Publicado 2020
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The study aimed to evaluate the presence of bacteria (total and thermotolerant coliforms) in the treatment of water using Class C Ultraviolet Radiation (UV-C), the research was carried out in the Quality Control Laboratory of the Treatment Plant of Potable Water of the EPS, EMSA Puno, because current regulations require that total and thermotolerant coliform readings in drinking water should be 0 CFU / 100 ml, with UV-C radiation being an alternative for the decrease in Chlorine use. For what was experimented with a dose of 0,00176 W/cm2/s of UV-C radiation with a dominant wavelength of 254 nm, the experimental water samples were subjected to 1, 2, 3, and 4 seconds of exposure to the mentioned radiation. For the determination of the bacterial presence in water (total and thermotolerant coliforms), the Membrane Filter methodology was chosen, the results obtained fo...
20
artículo
Publicado 2020
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Objective: The purpose of this study was to determinate the bacterial contamination of Beta (β) gutta-percha cones at 0, 24, 47 and 72 hours of the different supplies of the Universidad Científica del Sur, Lima 2020. Materials and Methods: 16 β-type gutta-percha cones were obtained of sealed packages under aseptic measurements, they were placed in vials with 2ml BHI and subsequently planted in BHI agar plates as well as in selective medias as Salted mannitol agar and MacConkey agar after 24 hours of incubation at 37 ° the plates were read and count in CFU , the same procedure was performed for the other times evaluated 24, 48 and 72 hours, giving a total of 64 β-type gutta-percha cones. Results: It was observed that the level of bacterial contamination was the same among the different supplies in all the establish times of in this study 0, 24, 48 and 72 hours. Therefore, there...