Subchronic Infection of porphyromonas gingivalis and tannerella forsythia stimulates an immune response but not arthritis in experimental murine model

• Autores: Hernández-Aguas, Jorday, Montiel-Hernández, José Luis, De La Garza-Ramos, Myriam A., Ruiz-Ramos, Rosa Velia, Escamilla García, Erandi, Guzmán-García, Mario Alberto, Ayón-Haro, Esperanza Raquel, Garza-Elizondo, Mario Alberto
• Formato: artículo
• Fecha de Publicación: 2017
• Institución: Universidad de San Martín de Porres
• Repositorio: USMP-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.usmp.edu.pe:20.500.12727/6136
• Enlace del recurso: https://hdl.handle.net/20.500.12727/6136; https://doi.org/10.1155/2017/2052938
• Nivel de acceso: acceso abierto
• Materia: Infecciones; Porphyromonas gingivalis; Tannerella forsythia; Artritis reumatoide; https://purl.org/pe-repo/ocde/ford#3.02.00

Studies have proposed that Porphyromonas gingivalis (Pg) and Tannerella forsythia (Tf) promote a nonspecific inflammatory response that could produce systemic disease. Oral inoculation of Pg and Tf on the immune and arthritis response was evaluated in BALB/C mice divided into four groups: (1) sham; (2) food contaminated with Pg/Tf; (3) complete Freund’s adjuvant (CFA) + Pg/Tf; and (4) CFA alone. CFA was administered subcutaneously on days 1 and 14. The arthritis response was monitored for 21 days after day 14 of CFA administration. IL-1β and IL-6 were determined in serum. T cell activation was evaluated by CD25 in salivary lymph nodes or mouse spleen. Pad inflammation appeared by day 19 in the CFA group, but animals with bacteria inoculation presented a delay. A significant increase in IL-6 was found in Groups 3 and 4, but not with respect to IL-1β. We observed an increase in CD25 in cells derived from cervical nodes and in animals with bacteria inoculation and CFA. A local immune response was observed in mice inoculated with Pg and Tf (T cell activation); a systemic response was observed with CFA. Since pad inflammation was delayed by bacterial inoculation this suggests that local T cell activation could decrease pad inflammation.