In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center
Descripción del Articulo
The present study evaluated the in vitro probiotic capacity of 150 lactic acid bacteria (BAL) isolated from feces of three breeds of guinea pigs from an Experimental Center of intensive rearing, by means of in vitro tests on agar that evaluated the capacity of amylolytic, proteolytic enzymatic activ...
| Autores: | , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2021 |
| Institución: | Universidad Peruana Cayetano Heredia |
| Repositorio: | Revistas - Universidad Peruana Cayetano Heredia |
| Lenguaje: | español |
| OAI Identifier: | oai:revistas.upch.edu.pe:article/3871 |
| Enlace del recurso: | https://revistas.upch.edu.pe/index.php/STV/article/view/3871 |
| Nivel de acceso: | acceso abierto |
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In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental CenterEvaluación In Vitro de la capacidad probiótica de bacterias ácido lácticas aisladas de heces de cuyes (Cavia porcellus) de un Centro ExperimentalSerrano, CarlaJara, Luis M.Chauca, LiliaShiva, CarlosThe present study evaluated the in vitro probiotic capacity of 150 lactic acid bacteria (BAL) isolated from feces of three breeds of guinea pigs from an Experimental Center of intensive rearing, by means of in vitro tests on agar that evaluated the capacity of amylolytic, proteolytic enzymatic activity, cellulolytic and antagonism against pathogenic Salmonella sp. The BAL strains that achieved a positive result in any of the in vitro tests were evaluated for survival at pH 2.0, 4.0 and 0.3% bile. Gram positive strains were obtained, 93.3% of them with bacillary morphology and6.7% with ovoid morphology. 38.7% of strains showed the ability to degrade soluble starch, 66.7% for milk proteins and 4% for sodium carboxymethyl cellulose. Only 5.3% of strains had an antagonistic action against Salmonella sp. The frequency of strains according to race in relation to amylolytic and proteolytic degradation proved to have a significant statistical association. The survival capacity of the strains evaluated by each breed is also highlighted, where a frequency greater than 40% was obtained in each trial. The probiotic potential of native BAL isolated from feces of guinea pigs that would be evaluated in future field trials is highlighted.El presente estudio evaluó la capacidad probiótica in vitro de 150 bacterias ácido lácticas (BAL) aisladas de heces de tres razas de cuyes provenientes de un Centro Experimental de crianza intensiva, mediante ensayos in vitro en agar que evaluaron la capacidad de actividad enzimática amilolítica, proteolítica, celulolítica y antagonismo frente a Salmonella sp. patogénica. Las cepas BAL que lograron algún resultado positivo a alguno de los ensayos in vitro fueron evaluadas para la supervivencia a pH 2.0, 4.0 y bilis al 0.3%. Se obtuvieron cepas Gram positivas, 93.3% de ellas con morfología bacilar y 6.7% con morfología ovoide. El 38.7% de cepas demostró capacidad de degradar almidón soluble, el 66.7% para proteínas de leche y el 4% para carboximetilcelulosa sódica. Solo el 5.3% de cepas tuvo acción antagónica frente a Salmonella sp. La frecuencia de cepas según raza en relación a la degradación amilolítica y proteolítica demostró tener asociación estadística significativa. Se destaca además la capacidad de supervivencia de las cepas evaluadas por cada raza, donde se obtuvo en cada ensayo una frecuencia mayor al 40%. Se resalta el potencial probiótico de BAL autóctonas aisladas de heces de cuyes que podrían ser evaluadas a futuro en ensayos de campo.Universidad Peruana Cayetano Heredia2021-01-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.upch.edu.pe/index.php/STV/article/view/387110.20453/stv.v8i2.3871Salud y Tecnología Veterinaria; Vol. 8 No. 2 (2020): July - December; 40-46Salud y Tecnología Veterinaria; Vol. 8 Núm. 2 (2020): Julio - Diciembre; 40-462312-3907reponame:Revistas - Universidad Peruana Cayetano Herediainstname:Universidad Peruana Cayetano Herediainstacron:UPCHspahttps://revistas.upch.edu.pe/index.php/STV/article/view/3871/4388https://revistas.upch.edu.pe/index.php/STV/article/view/3871/4389Derechos de autor 2021 Carla Serrano, Luis M. Jara, Lilia Chauca, Carlos Shivainfo:eu-repo/semantics/openAccessoai:revistas.upch.edu.pe:article/38712021-06-22T14:01:46Z |
| dc.title.none.fl_str_mv |
In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center Evaluación In Vitro de la capacidad probiótica de bacterias ácido lácticas aisladas de heces de cuyes (Cavia porcellus) de un Centro Experimental |
| title |
In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center |
| spellingShingle |
In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center Serrano, Carla |
| title_short |
In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center |
| title_full |
In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center |
| title_fullStr |
In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center |
| title_full_unstemmed |
In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center |
| title_sort |
In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center |
| dc.creator.none.fl_str_mv |
Serrano, Carla Jara, Luis M. Chauca, Lilia Shiva, Carlos |
| author |
Serrano, Carla |
| author_facet |
Serrano, Carla Jara, Luis M. Chauca, Lilia Shiva, Carlos |
| author_role |
author |
| author2 |
Jara, Luis M. Chauca, Lilia Shiva, Carlos |
| author2_role |
author author author |
| description |
The present study evaluated the in vitro probiotic capacity of 150 lactic acid bacteria (BAL) isolated from feces of three breeds of guinea pigs from an Experimental Center of intensive rearing, by means of in vitro tests on agar that evaluated the capacity of amylolytic, proteolytic enzymatic activity, cellulolytic and antagonism against pathogenic Salmonella sp. The BAL strains that achieved a positive result in any of the in vitro tests were evaluated for survival at pH 2.0, 4.0 and 0.3% bile. Gram positive strains were obtained, 93.3% of them with bacillary morphology and6.7% with ovoid morphology. 38.7% of strains showed the ability to degrade soluble starch, 66.7% for milk proteins and 4% for sodium carboxymethyl cellulose. Only 5.3% of strains had an antagonistic action against Salmonella sp. The frequency of strains according to race in relation to amylolytic and proteolytic degradation proved to have a significant statistical association. The survival capacity of the strains evaluated by each breed is also highlighted, where a frequency greater than 40% was obtained in each trial. The probiotic potential of native BAL isolated from feces of guinea pigs that would be evaluated in future field trials is highlighted. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-01-25 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.upch.edu.pe/index.php/STV/article/view/3871 10.20453/stv.v8i2.3871 |
| url |
https://revistas.upch.edu.pe/index.php/STV/article/view/3871 |
| identifier_str_mv |
10.20453/stv.v8i2.3871 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistas.upch.edu.pe/index.php/STV/article/view/3871/4388 https://revistas.upch.edu.pe/index.php/STV/article/view/3871/4389 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2021 Carla Serrano, Luis M. Jara, Lilia Chauca, Carlos Shiva info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2021 Carla Serrano, Luis M. Jara, Lilia Chauca, Carlos Shiva |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf text/html |
| dc.publisher.none.fl_str_mv |
Universidad Peruana Cayetano Heredia |
| publisher.none.fl_str_mv |
Universidad Peruana Cayetano Heredia |
| dc.source.none.fl_str_mv |
Salud y Tecnología Veterinaria; Vol. 8 No. 2 (2020): July - December; 40-46 Salud y Tecnología Veterinaria; Vol. 8 Núm. 2 (2020): Julio - Diciembre; 40-46 2312-3907 reponame:Revistas - Universidad Peruana Cayetano Heredia instname:Universidad Peruana Cayetano Heredia instacron:UPCH |
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Universidad Peruana Cayetano Heredia |
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UPCH |
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UPCH |
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Revistas - Universidad Peruana Cayetano Heredia |
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Revistas - Universidad Peruana Cayetano Heredia |
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Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).