In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center

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The present study evaluated the in vitro probiotic capacity of 150 lactic acid bacteria (BAL) isolated from feces of three breeds of guinea pigs from an Experimental Center of intensive rearing, by means of in vitro tests on agar that evaluated the capacity of amylolytic, proteolytic enzymatic activ...

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Autores: Serrano, Carla, Jara, Luis M., Chauca, Lilia, Shiva, Carlos
Formato: artículo
Fecha de Publicación:2021
Institución:Universidad Peruana Cayetano Heredia
Repositorio:Revistas - Universidad Peruana Cayetano Heredia
Lenguaje:español
OAI Identifier:oai:revistas.upch.edu.pe:article/3871
Enlace del recurso:https://revistas.upch.edu.pe/index.php/STV/article/view/3871
Nivel de acceso:acceso abierto
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spelling In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental CenterEvaluación In Vitro de la capacidad probiótica de bacterias ácido lácticas aisladas de heces de cuyes (Cavia porcellus) de un Centro ExperimentalSerrano, CarlaJara, Luis M.Chauca, LiliaShiva, CarlosThe present study evaluated the in vitro probiotic capacity of 150 lactic acid bacteria (BAL) isolated from feces of three breeds of guinea pigs from an Experimental Center of intensive rearing, by means of in vitro tests on agar that evaluated the capacity of amylolytic, proteolytic enzymatic activity, cellulolytic and antagonism against pathogenic Salmonella sp. The BAL strains that achieved a positive result in any of the in vitro tests were evaluated for survival at pH 2.0, 4.0 and 0.3% bile. Gram positive strains were obtained, 93.3% of them with bacillary morphology and6.7% with ovoid morphology. 38.7% of strains showed the ability to degrade soluble starch, 66.7% for milk proteins and 4% for sodium carboxymethyl cellulose. Only 5.3% of strains had an antagonistic action against Salmonella sp. The frequency of strains according to race in relation to amylolytic and proteolytic degradation proved to have a significant statistical association. The survival capacity of the strains evaluated by each breed is also highlighted, where a frequency greater than 40% was obtained in each trial. The probiotic potential of native BAL isolated from feces of guinea pigs that would be evaluated in future field trials is highlighted.El presente estudio evaluó la capacidad probiótica in vitro de 150 bacterias ácido lácticas (BAL) aisladas de heces de tres razas de cuyes provenientes de un Centro Experimental de crianza intensiva, mediante ensayos in vitro en agar que evaluaron la capacidad de actividad enzimática amilolítica, proteolítica, celulolítica y antagonismo frente a Salmonella sp. patogénica. Las cepas BAL que lograron algún resultado positivo a alguno de los ensayos in vitro fueron evaluadas para la supervivencia a pH 2.0, 4.0 y bilis al 0.3%. Se obtuvieron cepas Gram positivas, 93.3% de ellas con morfología bacilar y 6.7% con morfología ovoide. El 38.7% de cepas demostró capacidad de degradar almidón soluble, el 66.7% para proteínas de leche y el 4% para carboximetilcelulosa sódica. Solo el 5.3% de cepas tuvo acción antagónica frente a Salmonella sp. La frecuencia de cepas según raza en relación a la degradación amilolítica y proteolítica demostró tener asociación estadística significativa. Se destaca además la capacidad de supervivencia de las cepas evaluadas por cada raza, donde se obtuvo en cada ensayo una frecuencia mayor al 40%. Se resalta el potencial probiótico de BAL autóctonas aisladas de heces de cuyes que podrían ser evaluadas a futuro en ensayos de campo.Universidad Peruana Cayetano Heredia2021-01-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.upch.edu.pe/index.php/STV/article/view/387110.20453/stv.v8i2.3871Salud y Tecnología Veterinaria; Vol. 8 No. 2 (2020): July - December; 40-46Salud y Tecnología Veterinaria; Vol. 8 Núm. 2 (2020): Julio - Diciembre; 40-462312-3907reponame:Revistas - Universidad Peruana Cayetano Herediainstname:Universidad Peruana Cayetano Herediainstacron:UPCHspahttps://revistas.upch.edu.pe/index.php/STV/article/view/3871/4388https://revistas.upch.edu.pe/index.php/STV/article/view/3871/4389Derechos de autor 2021 Carla Serrano, Luis M. Jara, Lilia Chauca, Carlos Shivainfo:eu-repo/semantics/openAccessoai:revistas.upch.edu.pe:article/38712021-06-22T14:01:46Z
dc.title.none.fl_str_mv In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center
Evaluación In Vitro de la capacidad probiótica de bacterias ácido lácticas aisladas de heces de cuyes (Cavia porcellus) de un Centro Experimental
title In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center
spellingShingle In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center
Serrano, Carla
title_short In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center
title_full In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center
title_fullStr In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center
title_full_unstemmed In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center
title_sort In vitro evaluation of probiotic capability of acid lactic bacteria from guinea pig (Cavia porcellus) feces of an Experimental Center
dc.creator.none.fl_str_mv Serrano, Carla
Jara, Luis M.
Chauca, Lilia
Shiva, Carlos
author Serrano, Carla
author_facet Serrano, Carla
Jara, Luis M.
Chauca, Lilia
Shiva, Carlos
author_role author
author2 Jara, Luis M.
Chauca, Lilia
Shiva, Carlos
author2_role author
author
author
description The present study evaluated the in vitro probiotic capacity of 150 lactic acid bacteria (BAL) isolated from feces of three breeds of guinea pigs from an Experimental Center of intensive rearing, by means of in vitro tests on agar that evaluated the capacity of amylolytic, proteolytic enzymatic activity, cellulolytic and antagonism against pathogenic Salmonella sp. The BAL strains that achieved a positive result in any of the in vitro tests were evaluated for survival at pH 2.0, 4.0 and 0.3% bile. Gram positive strains were obtained, 93.3% of them with bacillary morphology and6.7% with ovoid morphology. 38.7% of strains showed the ability to degrade soluble starch, 66.7% for milk proteins and 4% for sodium carboxymethyl cellulose. Only 5.3% of strains had an antagonistic action against Salmonella sp. The frequency of strains according to race in relation to amylolytic and proteolytic degradation proved to have a significant statistical association. The survival capacity of the strains evaluated by each breed is also highlighted, where a frequency greater than 40% was obtained in each trial. The probiotic potential of native BAL isolated from feces of guinea pigs that would be evaluated in future field trials is highlighted.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.upch.edu.pe/index.php/STV/article/view/3871
10.20453/stv.v8i2.3871
url https://revistas.upch.edu.pe/index.php/STV/article/view/3871
identifier_str_mv 10.20453/stv.v8i2.3871
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.upch.edu.pe/index.php/STV/article/view/3871/4388
https://revistas.upch.edu.pe/index.php/STV/article/view/3871/4389
dc.rights.none.fl_str_mv Derechos de autor 2021 Carla Serrano, Luis M. Jara, Lilia Chauca, Carlos Shiva
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2021 Carla Serrano, Luis M. Jara, Lilia Chauca, Carlos Shiva
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidad Peruana Cayetano Heredia
publisher.none.fl_str_mv Universidad Peruana Cayetano Heredia
dc.source.none.fl_str_mv Salud y Tecnología Veterinaria; Vol. 8 No. 2 (2020): July - December; 40-46
Salud y Tecnología Veterinaria; Vol. 8 Núm. 2 (2020): Julio - Diciembre; 40-46
2312-3907
reponame:Revistas - Universidad Peruana Cayetano Heredia
instname:Universidad Peruana Cayetano Heredia
instacron:UPCH
instname_str Universidad Peruana Cayetano Heredia
instacron_str UPCH
institution UPCH
reponame_str Revistas - Universidad Peruana Cayetano Heredia
collection Revistas - Universidad Peruana Cayetano Heredia
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