Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru
Descripción del Articulo
The aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in...
| Autores: | , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2020 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/19044 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19044 |
| Nivel de acceso: | acceso abierto |
| Materia: | Perkinsus Argopecten purpuratus PCR |
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Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern PeruEvaluación molecular de la presencia de Perkinsus spp en muestras de concha de abanico (Argopecten purpuratus) del norte del PerúVivanco S., ManuelaValera A., AdhemirLlanco A., LuisSerrano-Martínez, EnriquePerkinsusArgopecten purpuratusPCRPerkinsusArgopecten purpuratusPCRThe aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in 2015. The selected samples were gill tissues with histological lesions suggestive of infection with the protozoan (hemocytic infiltration and necrosis). DNA was extracted from the samples and specific primers were used to detect Perkinsus spp by PCR. In addition, the detection limit of the technique was evaluated. All samples were negative. It was determined that 1.63 ng was the least detectable template DNA in one of the positive controls.El objetivo de este estudio fue evaluar la presencia de Perkinsus spp, parásito de reporte obligatorio ante la OIE, en muestras de concha de abanico (Argopecten purpuratus) de Ancash, Perú, mediante la técnica de PCR. Se seleccionaron 56 muestras de las colectadas para un estudio previo en 2015. Las muestras seleccionadas fueron tejidos de branquia con lesiones histológicas sugestivas de infección con el protozoo (infiltración hemocítica y necrosis). Se extrajo el ADN de las muestras y se utilizaron cebadores específicos para detectar Perkinsus spp por PCR. Además, se evaluó el límite de detección de la técnica. Todas las muestras resultaron negativas. Se determinó que 1.63 ng fue la menor cantidad de DNA molde detectable en uno de los controles positivos.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-12-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1904410.15381/rivep.v31i4.19044Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e19044Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e190441682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19044/16141Derechos de autor 2020 Manuela Vivanco S., Adhemir Valera A., Luis Llanco A., Enrique Serrano-Martínezhttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/190442020-12-21T17:00:34Z |
| dc.title.none.fl_str_mv |
Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru Evaluación molecular de la presencia de Perkinsus spp en muestras de concha de abanico (Argopecten purpuratus) del norte del Perú |
| title |
Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru |
| spellingShingle |
Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru Vivanco S., Manuela Perkinsus Argopecten purpuratus PCR Perkinsus Argopecten purpuratus PCR |
| title_short |
Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru |
| title_full |
Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru |
| title_fullStr |
Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru |
| title_full_unstemmed |
Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru |
| title_sort |
Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru |
| dc.creator.none.fl_str_mv |
Vivanco S., Manuela Valera A., Adhemir Llanco A., Luis Serrano-Martínez, Enrique |
| author |
Vivanco S., Manuela |
| author_facet |
Vivanco S., Manuela Valera A., Adhemir Llanco A., Luis Serrano-Martínez, Enrique |
| author_role |
author |
| author2 |
Valera A., Adhemir Llanco A., Luis Serrano-Martínez, Enrique |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Perkinsus Argopecten purpuratus PCR Perkinsus Argopecten purpuratus PCR |
| topic |
Perkinsus Argopecten purpuratus PCR Perkinsus Argopecten purpuratus PCR |
| description |
The aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in 2015. The selected samples were gill tissues with histological lesions suggestive of infection with the protozoan (hemocytic infiltration and necrosis). DNA was extracted from the samples and specific primers were used to detect Perkinsus spp by PCR. In addition, the detection limit of the technique was evaluated. All samples were negative. It was determined that 1.63 ng was the least detectable template DNA in one of the positive controls. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-12-21 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19044 10.15381/rivep.v31i4.19044 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19044 |
| identifier_str_mv |
10.15381/rivep.v31i4.19044 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19044/16141 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e19044 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e19044 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
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Universidad Nacional Mayor de San Marcos |
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UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
