The aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in 2015. The selected samples were gill tissues with histological lesions suggestive of infection with the protozoan (hemocytic infiltration and necrosis). DNA was extracted from the samples and specific primers were used to detect Perkinsus spp by PCR. In addition, the detection limit of the technique was evaluated. All samples were negative. It was determined that 1.63 ng was the least detectable template DNA in one of the positive controls.

The aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in 2015. The selected samples were gill tissues with histological lesions suggestive of infection with the protozoan (hemocytic infiltration and necrosis). DNA was extracted from the samples and specific primers were used to detect Perkinsus spp by PCR. In addition, the detection limit of the technique was evaluated. All samples were negative. It was determined that 1.63 ng was the least detectable template DNA in one of the positive controls.

Se reporta el caso de una vaca Holstein primípara de 5 días posparto que presentó problemas de locomoción. Durante el examen físico, las constantes fisiológicas se encontraron dentro de los rangos normales; sin embargo, se observó una inflamación en la región proximal y medial de la tibia del miembro posterior derecho, la cual fue confirmada mediante palpación. Tres días después, la vaca mostró dificultad para levantarse y desplazarse. A nivel medial y proximal, se evidenció un aumento en la flacidez del músculo gastrocnemio y su tendón. Debido al empeoramiento de los problemas de locomoción y la salud general del animal, al quinto día se decidió proceder con el sacrificio y se realizó una necropsia, cuyo diagnóstico definitivo fue la ruptura del músculo gastrocnemio y de la porción distal del bíceps femoral.