Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria

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The aim of this study was to characterize the microbiota of the intestinal tract of robalo (Centropomus sp), from natural environment and captivity, and to isolate and identify bacteria with probiotic potential. The intestinal microbiota was characterized by metagenomics directed to the 16S rRNA gen...

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Detalles Bibliográficos
Autores: Zatán Valdiviezo, Adrian Edú, Castillo Chunga, Deysy, Castañeda Vargas, Arnaldo Edward, Feria Zevallos, Manuel Alberto, Toledo Valdiviezo, Odalis Epifanía, Aguilar Zavaleta, Jorge Luis, Cueva Távara, Mario David, Motte, Emmerik
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/16036
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16036
Nivel de acceso:acceso abierto
Materia:Centropomus
microbiota
metagenomics
probiotics
metagenómica
probióticos
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spelling Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteriaCaracterización de la microbiota intestinal en robalo (Centropomus sp.) y aislamiento de bacterias probióticas potencialesZatán Valdiviezo, Adrian EdúCastillo Chunga, DeysyCastañeda Vargas, Arnaldo EdwardFeria Zevallos, Manuel AlbertoToledo Valdiviezo, Odalis EpifaníaAguilar Zavaleta, Jorge LuisCueva Távara, Mario DavidMotte, EmmerikCentropomusmicrobiotametagenomicsprobioticsCentropomusmicrobiotametagenómicaprobióticosThe aim of this study was to characterize the microbiota of the intestinal tract of robalo (Centropomus sp), from natural environment and captivity, and to isolate and identify bacteria with probiotic potential. The intestinal microbiota was characterized by metagenomics directed to the 16S rRNA gene. In addition, samples of intestinal mucus were cultured in MRS medium for bacterial isolation. Molecular identification was done by sequencing the 16S rRNA gene. Probiotic capabilities were evidenced by proteolytic and bacterial antagonism assays against Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii and Vibrio harveyi. The most abundant bacterial genera were Clostridium, Photobacterium, Cetobacterium and Rubritepida. Bacterial species, including Klebsiella sp, two strains of Weisiella cibaria and Lactococcus sp were isolated on MRS agar from the intestinal tract. W. cibaria strains showed broad antagonistic spectrum and positive proteolytic activity.El objetivo del estudio fue caracterizar la microbiota del tracto intestinal de robalo (Centropomus sp), proveniente de medio natural y cautiverio, además de aislar e identificar bacterias con potencial probiótico. La microbiota intestinal se caracterizó mediante metagenómica dirigida al gen 16S ARNr. Además, se sembraron muestras de mucus intestinal en medio de cultivo MRS para el aislamiento bacteriano. La identificación molecular se hizo mediante la secuenciación del gen 16S ARNr. Las capacidades probióticas se evidenciaron mediante ensayos proteolíticos y de antagonismo bacteriano frente a Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii y Vibrio harveyi. Los géneros bacterianos con mayor abundancia fueron Clostridium, Photobacterium, Cetobacterium y Rubritepida. Las especies bacterianas, incluyendo Klebsiella sp, dos cepas de Weisiella cibaria y Lactococcus sp fueron aisladas en agar MRS a partir del tracto intestinal. Las cepas W. cibaria mostraron amplio espectro antagonista y actividad proteolítica positiva.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-08-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1603610.15381/rivep.v31i3.16036Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 3 (2020); e16036Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 3 (2020); e160361682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16036/15506Derechos de autor 2020 Adrian Edú Zatán Valdiviezo, Deysy Castillo Chunga, Arnaldo Edward Castañeda Vargas, Manuel Alberto Feria Zevallos, Odalis Epifanía Toledo Valdiviezo, Jorge Luis Aguilar Zavaleta, Mario David Cueva Távara, Emmerik Mottehttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/160362020-08-11T16:20:53Z
dc.title.none.fl_str_mv Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria
Caracterización de la microbiota intestinal en robalo (Centropomus sp.) y aislamiento de bacterias probióticas potenciales
title Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria
spellingShingle Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria
Zatán Valdiviezo, Adrian Edú
Centropomus
microbiota
metagenomics
probiotics
Centropomus
microbiota
metagenómica
probióticos
title_short Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria
title_full Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria
title_fullStr Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria
title_full_unstemmed Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria
title_sort Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria
dc.creator.none.fl_str_mv Zatán Valdiviezo, Adrian Edú
Castillo Chunga, Deysy
Castañeda Vargas, Arnaldo Edward
Feria Zevallos, Manuel Alberto
Toledo Valdiviezo, Odalis Epifanía
Aguilar Zavaleta, Jorge Luis
Cueva Távara, Mario David
Motte, Emmerik
author Zatán Valdiviezo, Adrian Edú
author_facet Zatán Valdiviezo, Adrian Edú
Castillo Chunga, Deysy
Castañeda Vargas, Arnaldo Edward
Feria Zevallos, Manuel Alberto
Toledo Valdiviezo, Odalis Epifanía
Aguilar Zavaleta, Jorge Luis
Cueva Távara, Mario David
Motte, Emmerik
author_role author
author2 Castillo Chunga, Deysy
Castañeda Vargas, Arnaldo Edward
Feria Zevallos, Manuel Alberto
Toledo Valdiviezo, Odalis Epifanía
Aguilar Zavaleta, Jorge Luis
Cueva Távara, Mario David
Motte, Emmerik
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Centropomus
microbiota
metagenomics
probiotics
Centropomus
microbiota
metagenómica
probióticos
topic Centropomus
microbiota
metagenomics
probiotics
Centropomus
microbiota
metagenómica
probióticos
description The aim of this study was to characterize the microbiota of the intestinal tract of robalo (Centropomus sp), from natural environment and captivity, and to isolate and identify bacteria with probiotic potential. The intestinal microbiota was characterized by metagenomics directed to the 16S rRNA gene. In addition, samples of intestinal mucus were cultured in MRS medium for bacterial isolation. Molecular identification was done by sequencing the 16S rRNA gene. Probiotic capabilities were evidenced by proteolytic and bacterial antagonism assays against Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii and Vibrio harveyi. The most abundant bacterial genera were Clostridium, Photobacterium, Cetobacterium and Rubritepida. Bacterial species, including Klebsiella sp, two strains of Weisiella cibaria and Lactococcus sp were isolated on MRS agar from the intestinal tract. W. cibaria strains showed broad antagonistic spectrum and positive proteolytic activity.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16036
10.15381/rivep.v31i3.16036
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16036
identifier_str_mv 10.15381/rivep.v31i3.16036
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16036/15506
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 3 (2020); e16036
Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 3 (2020); e16036
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.87752
Characterization of the intestinal microbiota in snook (Centropomus sp.) and isolation of potential probiotic bacteria
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