Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System

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Stevia (Stevia rebaudiana A Bertoni), is a native species from Paraguay and Brazil, which is used as a natural sweetener with medicinal value in the preparation of beverages, sweets and others. Its propagation through sexual seeds is limited by their poor quality, for this reason its asexual propaga...

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Detalles Bibliográficos
Autores: Tapia y Figueroa, María de Lourdes, Gómez Pando, Luz R.
Formato: artículo
Fecha de Publicación:2022
Institución:Universidad Nacional Agraria La Molina
Repositorio:Revistas - Universidad Nacional Agraria La Molina
Lenguaje:inglés
OAI Identifier:oai:revistas.lamolina.edu.pe:article/1980
Enlace del recurso:https://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1980
Nivel de acceso:acceso abierto
Materia:sweetener
in vitro
bioreactor
genotype
Stevia
edulcorante
biorreactor
genotipo
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spelling Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor SystemPropagación clonal de líneas avanzadas de Stevia (Stevia rebaudiana Bertoni) en un sistema de Biorreactores de Inmersión TemporalTapia y Figueroa, María de Lourdes Gómez Pando, Luz R. Tapia y Figueroa, María de Lourdes Gómez Pando, Luz R. sweetenerin vitrobioreactorgenotypeSteviaedulcorantein vitrobiorreactorgenotipoSteviaStevia (Stevia rebaudiana A Bertoni), is a native species from Paraguay and Brazil, which is used as a natural sweetener with medicinal value in the preparation of beverages, sweets and others. Its propagation through sexual seeds is limited by their poor quality, for this reason its asexual propagation is promoted through different in vitro techniques. In the present investigation, the response of three genotypes of stevia to propagation in Temporary Immersion Bioreactor (TIB) System was studied with four different media as a treatments. The results showed that the treatment M2 (MS + 1 mg/L BAP + 0.1mg/L NAA + 30 g Sucrose) gave the best results for all evaluated characteristics with the exception of the number of rootlets/seedling and being the treatment M1 (MS without growth regulators + 30 g of Sucrose) achieved the highest number of roots and good values for the other characteristics. High quality plants were achieved and the genotype that responded best was IBT-1, and in the acclimatization phase a high survival rate was obtained.La Stevia (Stevia rebaudiana Bertoni), es una especie originaria de Paraguay y Brasil, la cual se utiliza como edulcorante natural con valor medicinal en la preparación de bebidas, dulces y otros. Su propagación a través de semillas sexuales está limitada por la mala calidad de las mismas, por lo que se promueve su propagación asexual mediante diferentes técnicas in vitro. En la presente investigación se estudió la respuesta de tres líneas avanzadas de stevia a la propagación en Biorreactores de Inmersión Temporal con cuatro tratamientos diferentes. El tratamiento M2 (MS + 1 mg/L BAP + 0.1 mg/L NAA + 30 g Sacarosa) dio los mejores resultados para todas las características evaluadas con excepción del número de raicillas/plántula y siendo el tratamiento M1 (MS sin reguladores de crecimiento + 30 g de Sacarosa) con la que se logró el mayor número, de raíces y buenos valores para las demás características. Se lograron plantas de alta calidad y el genotipo que mejor respondió fue IBT-1 y en la fase de aclimatación se obtuvo una alta tasa de supervivencia.Universidad Nacional Agraria La Molina2022-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/198010.21704/pja.v6i3.1980Peruvian Journal of Agronomy; Vol. 6 No. 3 (2022): September to December; 256-262Peruvian Journal of Agronomy; Vol. 6 Núm. 3 (2022): Setiembre a Diciembre; 256-2622616-4477reponame:Revistas - Universidad Nacional Agraria La Molinainstname:Universidad Nacional Agraria La Molinainstacron:UNALMenghttps://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1980/2522Derechos de autor 2022 María de Lourdes Tapia y Figueroa, Luz R. Gómez Pandohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:revistas.lamolina.edu.pe:article/19802025-04-23T16:36:05Z
dc.title.none.fl_str_mv Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System
Propagación clonal de líneas avanzadas de Stevia (Stevia rebaudiana Bertoni) en un sistema de Biorreactores de Inmersión Temporal
title Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System
spellingShingle Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System
Tapia y Figueroa, María de Lourdes
sweetener
in vitro
bioreactor
genotype
Stevia
edulcorante
in vitro
biorreactor
genotipo
Stevia
title_short Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System
title_full Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System
title_fullStr Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System
title_full_unstemmed Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System
title_sort Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System
dc.creator.none.fl_str_mv Tapia y Figueroa, María de Lourdes
Gómez Pando, Luz R.
Tapia y Figueroa, María de Lourdes
Gómez Pando, Luz R.
author Tapia y Figueroa, María de Lourdes
author_facet Tapia y Figueroa, María de Lourdes
Gómez Pando, Luz R.
author_role author
author2 Gómez Pando, Luz R.
author2_role author
dc.subject.none.fl_str_mv sweetener
in vitro
bioreactor
genotype
Stevia
edulcorante
in vitro
biorreactor
genotipo
Stevia
topic sweetener
in vitro
bioreactor
genotype
Stevia
edulcorante
in vitro
biorreactor
genotipo
Stevia
description Stevia (Stevia rebaudiana A Bertoni), is a native species from Paraguay and Brazil, which is used as a natural sweetener with medicinal value in the preparation of beverages, sweets and others. Its propagation through sexual seeds is limited by their poor quality, for this reason its asexual propagation is promoted through different in vitro techniques. In the present investigation, the response of three genotypes of stevia to propagation in Temporary Immersion Bioreactor (TIB) System was studied with four different media as a treatments. The results showed that the treatment M2 (MS + 1 mg/L BAP + 0.1mg/L NAA + 30 g Sucrose) gave the best results for all evaluated characteristics with the exception of the number of rootlets/seedling and being the treatment M1 (MS without growth regulators + 30 g of Sucrose) achieved the highest number of roots and good values for the other characteristics. High quality plants were achieved and the genotype that responded best was IBT-1, and in the acclimatization phase a high survival rate was obtained.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1980
10.21704/pja.v6i3.1980
url https://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1980
identifier_str_mv 10.21704/pja.v6i3.1980
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1980/2522
dc.rights.none.fl_str_mv Derechos de autor 2022 María de Lourdes Tapia y Figueroa, Luz R. Gómez Pando
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2022 María de Lourdes Tapia y Figueroa, Luz R. Gómez Pando
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Agraria La Molina
publisher.none.fl_str_mv Universidad Nacional Agraria La Molina
dc.source.none.fl_str_mv Peruvian Journal of Agronomy; Vol. 6 No. 3 (2022): September to December; 256-262
Peruvian Journal of Agronomy; Vol. 6 Núm. 3 (2022): Setiembre a Diciembre; 256-262
2616-4477
reponame:Revistas - Universidad Nacional Agraria La Molina
instname:Universidad Nacional Agraria La Molina
instacron:UNALM
instname_str Universidad Nacional Agraria La Molina
instacron_str UNALM
institution UNALM
reponame_str Revistas - Universidad Nacional Agraria La Molina
collection Revistas - Universidad Nacional Agraria La Molina
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.108393
Clonal propagation of Stevia (Stevia rebaudiana Bertoni) advanced lines in a Temporary Immersion Bioreactor System
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