Stevia (Stevia rebaudiana A Bertoni), is a native species from Paraguay and Brazil, which is used as a natural sweetener with medicinal value in the preparation of beverages, sweets and others. Its propagation through sexual seeds is limited by their poor quality, for this reason its asexual propagation is promoted through different in vitro techniques. In the present investigation, the response of three genotypes of stevia to propagation in Temporary Immersion Bioreactor (TIB) System was studied with four different media as a treatments. The results showed that the treatment M2 (MS + 1 mg/L BAP + 0.1mg/L NAA + 30 g Sucrose) gave the best results for all evaluated characteristics with the exception of the number of rootlets/seedling and being the treatment M1 (MS without growth regulators + 30 g of Sucrose) achieved the highest number of roots and good values for the other characteristic...

Stevia rebaudiana is considered an important medicinal plant possessing low-calorie glucoside sweeteners. The present work describes the comparison of three stevia genotypes (IBT 1, IBT 2 and IBT 3) in two contrasting environments simulated under controlled conditions: Sullana in Peru; and Misiones in Paraguay (regarded as the center of origin of Stevia). In the study, we explored the Euclidean distance as an integrating indicator for simultaneous selection of several stevia traits. Plant scientists often record multiple morphological, physiological and biochemical indicators in their experiments. Common statistical data evaluations involve univariate analyses such as t-test, Mann-Whitney and Analysis of Variance followed by Tukey HSD. However, these analyses do not evaluate integrally the effects of the experimental treatments because each indicator is analyzed independently. Euclidean ...