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Stevia rebaudiana is considered an important medicinal plant possessing low-calorie glucoside sweeteners. The present work describes the comparison of three stevia genotypes (IBT 1, IBT 2 and IBT 3) in two contrasting environments simulated under controlled conditions: Sullana in Peru; and Misiones in Paraguay (regarded as the center of origin of Stevia). In the study, we explored the Euclidean distance as an integrating indicator for simultaneous selection of several stevia traits. Plant scientists often record multiple morphological, physiological and biochemical indicators in their experiments. Common statistical data evaluations involve univariate analyses such as t-test, Mann-Whitney and Analysis of Variance followed by Tukey HSD. However, these analyses do not evaluate integrally the effects of the experimental treatments because each indicator is analyzed independently. Euclidean ...

Stevia (Stevia rebaudiana A Bertoni), is a native species from Paraguay and Brazil, which is used as a natural sweetener with medicinal value in the preparation of beverages, sweets and others. Its propagation through sexual seeds is limited by their poor quality, for this reason its asexual propagation is promoted through different in vitro techniques. In the present investigation, the response of three genotypes of stevia to propagation in Temporary Immersion Bioreactor (TIB) System was studied with four different media as a treatments. The results showed that the treatment M2 (MS + 1 mg/L BAP + 0.1mg/L NAA + 30 g Sucrose) gave the best results for all evaluated characteristics with the exception of the number of rootlets/seedling and being the treatment M1 (MS without growth regulators + 30 g of Sucrose) achieved the highest number of roots and good values for the other characteristic...

Universidad Nacional Agraria La Molina. Escuela de Posgrado. Doctorado en Agricultura Sustentable

En la propagación de bambúes, se utilizan generalmente métodos de reproducción asexual debido a que la floración de estas especies solo se presenta a intervalos o ciclos muy largos. La micro propagación es una alternativa para superar los problemas que se presentan en la propagación convencional de estas especies. El objetivo de este trabajo fue contribuir al desarrollo del protocolo para la propagación in vitro de Guadua weberbaueri, mediante la obtención de explantes libres de patógenos para su desarrollo en un medio de multiplicación. Se aplicaron tres tratamientos de desinfección usando alcohol al 70%, hipoclorito de sodio a diferentes concentraciones y Tween 20. El mejor tratamiento fue el tratamiento 3, que consistió en una limpieza con hipoclorito de sodio al 2,5% por 10 minutos, y una segunda desinfección con hipoclorito de sodio al 1,5% por 3 minutos. En la fase de...