Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR

• Autores: Castaneda, Carlos A, Castillo, Miluska, Sanchez, Joselyn, Casavilca, Sandro, Sanchez, Juvenal, Bernabe, Luis A, Suarez, Nancy, Chavez, Ivan, Ruiz, Eloy, Tello, Katherine, Villa, Maria R, Zevallos, Rocio, Montenegro, Paola, Dias-Neto, Emmanuel, Landa-Baella, Maria, Taxa, Luis
• Formato: artículo
• Fecha de Publicación: 2020
• Institución: Instituto Nacional de Enfermedades Neoplásicas
• Repositorio: INEN-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.inen.sld.pe:20.500.14703/498
• Enlace del recurso: https://hdl.handle.net/20.500.14703/498
• Nivel de acceso: acceso abierto
• Materia: Helicobacter pylori; PCR; Eosin; Hematoxylin; Histopathology; Immunohistochemistry; Stomach neoplasms; https://purl.org/pe-repo/ocde/ford#3.02.21

Aim:Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results:H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.