Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR

• Autores: A Castaneda C., Castillo M., Sanchez J., Casavilca S., A Bernabe L., Suarez N., Chavez I., Ruiz E., Tello K., R Villa M., Zevallos R., Montenegro P., Dias-Neto E., Landa-Baella M., Taxa L.
• Formato: artículo
• Fecha de Publicación: 2020
• Institución: Consejo Nacional de Ciencia Tecnología e Innovación
• Repositorio: CONCYTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.concytec.gob.pe:20.500.12390/2514
• Enlace del recurso: https://hdl.handle.net/20.500.12390/2514; https://doi.org/10.2217/fmb-2019-0280
• Nivel de acceso: acceso abierto
• Materia: stomach neoplasms; eosin; Helicobacter pylori; hematoxylin; histopathology; immunohistochemistry; PCR; http://purl.org/pe-repo/ocde/ford#3.04.02

Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results. © 2020 Future Medicine Ltd.. All rights reserved.